Effects of Sinusoidal Electromagnetic Field on Structure and Function of Different Kinds of Cell Lines

Abstract

This study investigated that whether a 2 mT, 60 Hz, sinusoidal electromagnetic field (EMF) alters the structure and function of cells. This research compared the effects of EMF on four kinds of cell lines: hFOB 1.19 (fetal osteoblast), T/G HA-VSMC (aortic vascular smooth muscle cell), RPMI 7666 (B lymphoblast), and HCN-2 (cortical neuronal cell). Over 14 days, cells were exposed to EMF for 1, 3, or 6 hours per day (hrs/d). The results pointed to a cell type-specific reaction to EMF exposure. In addition, the cellular responses were dependent on duration of EMF exposure. In the present study, cell proliferation was the trait most sensitive to EMF. EMF treatment promoted growth of hFOB 1.19 and HCN-2 compared with control cells at 7 and 14 days of incubation. When the exposure time was 3 hrs/d, EMF enhanced the proliferation of RPMI 7666 but inhibited that of T/G HA-VSMC. On the other hand, the effects of EMF on cell cycle distribution, cell differentiation, and actin distribution were unclear. Furthermore, we hardly found any correlation between EMF exposure and gap junctional intercellular communication in hFOB 1.19. This study revealed that EMF might serve as a potential tool for manipulating cell proliferation.

Effects of Electromagnetic Field (EMF) on Histological Changes and Norepinephrine Levels in the Brains of Adult Male Rats

“…Purkinje cell morphological changes may also be caused by decreased nuclear activities leading to reducing of cellular activities. These findings also reinforce other studies such as Sul et al (2006) and also Manti and Darco (2010). In the study Sul et al (2006), 2 mT EMF was induced into several cell types of cortical neurons, in vascular smooth muscle cells of aorta, fetal osteoblasts and limphoblast B.…”

“…These findings also reinforce other studies such as Sul et al (2006) and also Manti and Darco (2010). In the study Sul et al (2006), 2 mT EMF was induced into several cell types of cortical neurons, in vascular smooth muscle cells of aorta, fetal osteoblasts and limphoblast B. The study proves that EMF can manipulate cell proliferation and reduce the recovery of DNA.…”

Clinical significance of different effects of static and pulsed electromagnetic fields on human osteoclast cultures

“…This form of exogenous stimulation is known to affect bone metabolism by modifying some relevant physiologic cell parameters of cells, even though the underlying mechanisms are still unclear [27].…”

Antagonistic Effects of a 50 Hz Magnetic Field and Melatonin in the Proliferation and Differentiation of Hepatocarcinoma Cells

“…For instance, significantly increased growth rate and decreased proinflammatory chemokine production have been described on human keratinocytes (HaCaT) exposed to 50 Hz, 1 mT MF [59]. Other authors have described alterations in the kinetics of the cell cycle and/or in its regulatory proteins, under exposure to ELF fields [4,60,61]. Also, Ke et al [62] reported EGF-receptor clustering and Ras activation in Chinese hamster lung fibroblasts exposed to 400 μT MF.…”

“…Sul et al [61] reported that 3 h/day exposure to a 60 Hz, 2 mT MF stimulates proliferation in the lymphoblastic cell line RPMI 7666, but inhibits proliferation in muscle cells T/G HA-VSCM. By contrast, pulse wave, 16 -80 Hz MF at 1.55 mT did not affect significantly proliferation in HepG2 [63].This specificity in the cellular response could explain in part the inhomogeneity in results reported by different authors using different exposure conditions or different in vitro models, as proposed by Ross [64] or Zhang et al [65].…”