Effects of single-walled carbon nanotubes on the polymerase chain reaction

Cui, Daxiang; Tian, Furong; Kong, Yong; Titushikin, Igor; Gao, Huajian

doi:10.1088/0957-4484/15/1/030

Cited by 126 publications

(121 citation statements)

References 21 publications

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“…It has been described that nanotubes exhibit various effects on biomolecules, such as stimulation as well as inhibition of the polymerase chain reaction. 49 Moreover, studies with living organisms indicate adverse effects for CNTs in mice and rats, 21,23,24 and a comparable cell agglomeration in vitro has been shown here ( Figure 2B). The lung granuloma as well as the accumulation of cells in culture in close proximity of larger CNT bundles suggest an attractive role of CNT surfaces without acute toxicity.…”

supporting

confidence: 79%

Oops They Did It Again! Carbon Nanotubes Hoax Scientists in Viability Assays

2006

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New materials of emerging technological importance are single-walled carbon nanotubes (SWCNTs). Because SWCNTs will be used in commercial products in huge amounts, their effects on human health and the environment have been addressed in several studies. Inhalation studies in vivo and submerse applications in vitro have been described with diverging results. Why some indicate a strong cytotoxicity and some do not is what we report on here. Data from A549 cells incubated with carbon nanotubes fake a strong cytotoxic effect within the MTT assay after 24 h that reaches roughly 50%, whereas the same treatment with SWCNTs, but detection with WST-1, reveals no cytotoxicity. LDH, FACSassisted mitochondrial membrane potential determination, and Annexin-V/PI staining also reveal no cytotocicity. SWCNTs appear to interact with some tetrazolium salts such as MTT but not with others (such as WST-1, INT, XTT). This interference does not seem to affect the enzymatic reaction but lies rather in the insoluble nature of MTT−formazan. Our findings strongly suggest verifying cytotoxicity data with at least two or more independent test systems for this new class of materials (nanomaterials). Moreover, we intensely recommend standardizing nanotoxicological assays with regard to the material used: there is a clear need for reference materials. MTT−formazan crystals formed in the MTT reaction are lumped with nanotubes and offer a potential mechanism to guide bioremediation and clearance for SWCNTs from "contaminated" tissue. SWCNTs are good supporting materials for tissue growth, as attachment of focal adhesions and connections to the cytoskeleton suggest.

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supporting

confidence: 79%

Oops They Did It Again! Carbon Nanotubes Hoax Scientists in Viability Assays

2006

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“…Previous research has shown that different nanomaterials can strongly bind to DNA, increase the specificity and efficiency of PCR, and selectively induce special DNA conformation formation [23][24][25]. Our hypothesis is that the nanomaterials can bind to DNA and induce DNA damage, inhibit or improve DNA replication, or cause other unknown negative biological effects in vivo.…”

Section: Introductionmentioning

confidence: 87%

C60 affects DNA replication in vitro by decreasing the melting temperature of DNA templates

Liang

Luo

Lin

et al. 2009

Carbon

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A B S T R A C TThe effect of C 60 on DNA replication in vitro was studied by a quantitative real-time polymerase chain reaction (QPCR) system using a designed 110bp single-stranded DNA as the template. The results indicated that the efficiency of QPCR can be dramatically enhanced by C 60 at the beginning of the exponential phase, and that QPCR production can be significantly inhibited by C 60 at later cycles or at the plateau, which is the period represented by an interesting inverted U-shaped time response curve. Two different sized double-stranded DNA fragments (110bp and 60bp) were used to investigate the possible interaction between C 60 and DNA. The melting curve results showed that C 60 significantly decreased the melting temperatures (Tm) of the DNA fragments. By changing the concentrations of the initial DNA templates or C 60 in the QPCR system, we found that the decreased Tm value of DNA templates by C 60 is the primary reason for the increased QPCR efficiency. Our findings are consistent with the conclusions of other studies that C 60 can disrupt DNA replication in vitro by binding to DNA and changing the conformation of DNA templates.

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“…To date, there have been some reports closely associated with the methods of improving PCR, including optimizing reaction condition, caring for primer design, adjusting concentration of Mg 2+ ions, selecting high-quality Taq enzyme, using hot-start procedures, and using advanced PCR machines such as the capillary PCR machine [18]. The previous reports showed that singlewalled carbon nanotubes can improve the efficiency and specificity of PCR [19][20], mercaptoacetic acidmodified CdTe uantum dots can improve the specificity of PCR [21]. However, the concrete mechanism is not still clarified well.…”

Section: Open Accessmentioning

confidence: 99%

Real time PCR based on Fluorescent Quenching of Mercaptoacetic Acid-Modified CdTe Quantum Dots for Ultrasensitive Specific Detection of Nucleic Acids

Cui¹,

Li²,

Huang³

et al. 2010

Nano BioMed ENG

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A ultra-sensitive, highly specific, real-time polymerase chain reaction system based on mercaptoacetic acid-modified CdTe nanocrystals(mQDs) is reported. With the addition of 3 nm mQDs into the PCR reagent, the photoluminescent(PL) intensities of mQDs decreased gradually as the DNA templates and PCR cycles increased, in an approximate negative linear relation to the DNA concentration logarithm or cycles, the PL peaks exhibited red-shifts synchronously. Mg 2+ ions decreased the PL intensity of mQDs in a dose-dependent means, and Taq DNA polymerase enhanced the PL intensity of mQDs in a dose-dependent means. Real-time PCR based on mQDs showed an increased sensitivity at least 103 fold higher than that based on SYBR Green I. The specificity of PCR was enhanced in the PCR reagent with less than 1.33mg/mL mQDs. The potential mechanism is also discussed. This novel PCR system based on mQDs has great potential in applications such as ultra-sensitive specific DNA or RNA detection, dynamic molecular imaging, and photoelectric biosensors.

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Effects of single-walled carbon nanotubes on the polymerase chain reaction

Cited by 126 publications

References 21 publications

Oops They Did It Again! Carbon Nanotubes Hoax Scientists in Viability Assays

Oops They Did It Again! Carbon Nanotubes Hoax Scientists in Viability Assays

C60 affects DNA replication in vitro by decreasing the melting temperature of DNA templates

Real time PCR based on Fluorescent Quenching of Mercaptoacetic Acid-Modified CdTe Quantum Dots for Ultrasensitive Specific Detection of Nucleic Acids

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