Effects of single and combined low frequency electromagnetic fields and simulated microgravity on gene expression of human mesenchymal stem cells during chondrogenesis

Mayer-Wagner, Susanne; Hammerschmid, Florian; Blum, Helmut; Krebs, Stefan; Redeker, Julia I.; Holzapfel, Boris Michael; Jansson, Volkmar; Müller, Peter E.

doi:10.5114/aoms.2016.59894

Cited by 13 publications

(5 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shorter SMG treatments appeared to inhibit osteogenesis[ 47 - 49 ] and promote endothelial cell differentiation[ 48 ], neuronal differentiation[ 44 , 48 ], and adipogenic differentiation[ 48 , 49 ]. However, extended SMG decreased the potential for chondrogenic differentiation in MSCs[ 50 ] and encouraged their differentiation toward osteogenesis[ 46 , 48 ]. Different SMG action times had different effects on the cytoskeleton and could even lead to the aforementioned changes through different signal transduction pathways.…”

Section: Physical Stimulimentioning

confidence: 99%

“…In fact, the actual microenvironment in which cells were exposed was multifactorial. Therefore, some studies are now starting to consider the effect of compound factors[ 50 , 55 , 61 , 66 ] on the behavior of MSCs. Compound factors could have synergistic effects that increase the benefits for MSCs or counteract the drawbacks of a single factor.…”

Section: Physical Stimulimentioning

confidence: 99%

See 1 more Smart Citation

Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells

Zhou¹,

Wan²,

Wang³

et al. 2023

World J Stem Cells

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs), distributed in many tissues in the human body, are multipotent cells capable of differentiating in specific directions. It is usually considered that the differentiation process of MSCs depends on specialized external stimulating factors, including cell signaling pathways, cytokines, and other physical stimuli. Recent findings have revealed other underrated roles in the differentiation process of MSCs, such as material morphology and exosomes. Although relevant achievements have substantially advanced the applicability of MSCs, some of these regulatory mechanisms still need to be better understood. Moreover, limitations such as long-term survival in vivo hinder the clinical application of MSCs therapy. This review article summarizes current knowledge regarding the differentiation patterns of MSCs under specific stimulating factors.

show abstract

Section: Physical Stimulimentioning

confidence: 99%

Section: Physical Stimulimentioning

confidence: 99%

Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells

Zhou¹,

Wan²,

Wang³

et al. 2023

World J Stem Cells

View full text Add to dashboard Cite

show abstract

“…In this context, the exposure to the electromagnetic fields (EMFs) of cells grown in 3D cell systems is gaining a growing interest (mainly for tissue regeneration purposes), although the experimental data are still very limited [ 18 – 20 ]. A few reports characterized the response to the extremely low-frequency (ELF) magnetic field (MF) in both primary bovine chondrocytes and mesenchymal stem cells grown in 3D cultures to stimulate chondrogenesis and cartilage maturation [ 21 , 22 ], or in epidermal stem cells seeded in collagen sponge scaffolds for improving wound healing [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two-Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures

et al. 2020

View full text Add to dashboard Cite

We here characterize the response to the extremely low-frequency (ELF) magnetic field (MF, 50 Hz, 1 mT) of SH-SY5Y human neuroblastoma cells, cultured in a three-dimensional (3D) Alvetex® scaffold compared to conventional two-dimensional (2D) monolayers. We proved that the growing phenotype of proliferating SH-SY5Y cells is not affected by the culturing conditions, as morphology, cell cycle distribution, proliferation/differentiation gene expression of 3D-cultures overlap what reported in 2D plates. In response to 72-h exposure to 50-Hz MF, we demonstrated that no proliferation change and apoptosis activation occur in both 2D and 3D cultures. Consistently, no modulation of Ki67, MYCN, CCDN1, and Nestin, of invasiveness and neo-angiogenesis-controlling genes (HIF-1α, VEGF, and PDGF) and of microRNA epigenetic signature (miR-21-5p, miR-222-3p and miR-133b) is driven by ELF exposure. Conversely, intracellular glutathione content and SOD1 expression are exclusively impaired in 3D-culture cells in response to the MF, whereas no change of such redox modulators is observed in SH-SY5Y cells if grown on 2D monolayers. Moreover, ELF-MF synergizes with the differentiating agents to stimulate neuroblastoma differentiation into a dopaminergic (DA) phenotype in the 3D-scaffold culture only, as growth arrest and induction of p21, TH, DAT, and GAP43 are reported in ELF-exposed SH-SY5Y cells exclusively if grown on 3D scaffolds. As overall, our findings prove that 3D culture is a more reliable experimental model for studying SH-SY5Y response to ELF-MF if compared to 2D conventional monolayer, and put the bases for promoting 3D systems in future studies addressing the interaction between electromagnetic fields and biological systems.

show abstract

“…The effects of magnetic fields influence biological activities [ 31 ], not only in osteoporosis [ 32 ] and orthodontics [ 33 ] but also with an active role in cartilage tissue engineering [ 34 ]. Related studies have provided evidence that moderate-intensity SMF can effectively promote chondrogenic differentiation of BMSCs [ 10 , 25 ]. Our earlier study tested different SMF strengths by exposing MCCs to 160, 280, or 360 mT and found that the proliferation and chondrogenesis-promoting effects of 280 mT SMF were the most pronounced [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…The induction of BMSC chondrogenesis to repair articular cartilage defects has recently begun to be intensively studied [ 9 ]. Many studies focused on BMSCs from the femur and tibia [ 10 ]; however, few studies were interested in the mandibular BMSCs, which contribute to mandible development. At the 7 th week of the embryo [ 11 ], MBMSCs located at the base of the mandible gradually coagulated into a mass, and then the central cells of the mass differentiated into osteoblasts, finally forming the mandible by intramembrane osteogenesis.…”

Section: Introductionmentioning

confidence: 99%

Static Magnetic Fields Enhance the Chondrogenesis of Mandibular Bone Marrow Mesenchymal Stem Cells in Coculture Systems

Zhang

et al. 2021

BioMed Research International

View full text Add to dashboard Cite

Objectives. Combining the advantages of static magnetic fields (SMF) and coculture systems, we investigated the effect of moderate-intensity SMF on the chondrogenesis and proliferation of mandibular bone marrow mesenchymal stem cells (MBMSCs) in the MBMSC/mandibular condylar chondrocyte (MCC) coculture system. The main aim of the present study was to provide an experimental basis for obtaining better cartilage tissue engineering seed cells for the effective repair of condylar cartilage defects in clinical practice. Methods. MBMSCs and MCCs were isolated from SD (Sprague Dawley) rats. Flow cytometry, three-lineage differentiation, colony-forming assays, immunocytochemistry, and toluidine blue staining were used for the identification of MBMSCs and MCCs. MBMSCs and MCCs were seeded into the lower and upper Transwell chambers, respectively, at a ratio of 1 : 2, and exposed to a 280 mT SMF. MBMSCs were harvested after 3, 7, or 14 days for analysis. CCK-8 was used to detect cell proliferation, Alcian blue staining was utilized to evaluate glycosaminoglycan (GAG), and western blotting and real-time quantitative polymerase chain reaction (RT-qPCR) detected protein and gene expression levels of SOX9, Col2A1 (Collagen Type II Alpha 1), and Aggrecan (ACAN). Results. The proliferation of MBMSCs was significantly enhanced in the experimental group with MBMSCs cocultured with MCCs under SMF stimulation relative to controls ( P < 0.05 ). GAG content was increased, and SOX9, Col2A1, and ACAN were also increased at the mRNA and protein levels ( P < 0.05 ). Conclusions. Moderate-intensity SMF improved the chondrogenesis and proliferation of MBMSCs in the coculture system, and it might be a promising approach to repair condylar cartilage defects in the clinical setting.

show abstract

Effects of single and combined low frequency electromagnetic fields and simulated microgravity on gene expression of human mesenchymal stem cells during chondrogenesis

Cited by 13 publications

References 40 publications

Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells

Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells

Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two-Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures

Static Magnetic Fields Enhance the Chondrogenesis of Mandibular Bone Marrow Mesenchymal Stem Cells in Coculture Systems

Contact Info

Product

Resources

About