Effects of simulated microgravity on mammalian fertilization and preimplantation embryonic development in vitro

Kojima, Yoshiyuki; Sasaki, Shoichi; Kubota, Yasue; Ikeuchi, Takahito; Hayashi, Yutaro; Kohri, Kenjiro

doi:10.1016/s0015-0282(00)01583-1

Cited by 34 publications

(29 citation statements)

References 14 publications

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“…The results suggested that fertilization in vitro was not sensitive to the gravitational vector. However, the possibility still exists that the frequency of early embryonic lethality is increased by microgravity (Kojma et al 2000). We also studied simulated microgravity on preimplantation embryos' development using a rotating wall vessel bioreactor (RWVB); our study indicated that embryonic development was significantly retarded in RWVB.…”

Section: Introductionmentioning

confidence: 92%

Real-Time Micrography of Mouse Preimplantation Embryos in an Orbit Module on SJ-8 Satellite

Cao

Zheng

et al. 2008

Microgravity Sci. Technol

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The developmental capacity of mouse embryos in the Chinese SJ-8 Satellite was observed by real time micrography and telecontrol image transmission. Frozen/ thawed 4-cell embryos and blastocysts injected with mouse epidemical stem cells were placed in a specially sealed embryonic incubator, and then the incubator was loaded in a space embryonic culture box devised for space-flight. After the satellite launched and arrived at the anticipated orbit, the real time micrography device was opened based on the telecontrol operational technology. Real time micrographs of the mouse embryos were obtained and stored every 3 hours, then the data of images were transmitted at the suitable time. The experiment persisted for 72 hours. The results showed that during space-flight, most mouse embryos cultured in the sealed culture unit kept integrity and natural structure, their location had minor change, but the embryos did not develop. However, the experiment performed on the ground in the same device showed that 4-cell mouse embryos could develop to blastocysts and hatched blastocysts. It may be concluded that the space environment, especially the change of gravity was likely to harm development of the mouse embryo.

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Section: Introductionmentioning

confidence: 92%

Real-Time Micrography of Mouse Preimplantation Embryos in an Orbit Module on SJ-8 Satellite

Cao

Zheng

et al. 2008

Microgravity Sci. Technol

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“…There have been many studies done on embryos in microgravity and a variety of species have been used (Fritzsch and Bruce, 1995;Gaboyard et al, 2002;Gualandris-Parisot et al, 2001;Horneck, 1999;Ijiri, 1995;Kojima et al, 2000;Neff et al, 1993;Orban et al, 1999;Schatten et al, 1999). Comparing variations in embryo development over a broad range of species can be difficult.…”

Section: Embryos In Microgravitymentioning

confidence: 99%

“…Rats were allowed to mate in a Cosmos 1129 biosatellite experiment and the females failed to become pregnant, although fertilization had occurred (Serova et al, 1982). Development of mice in microgravity has not been successful to date when they were exposed to microgravity throughout the whole developmental period: Kojima et al (2000) noted that the pre-implantation embryos were resorbed. A flight of the space shuttle Columbia (STS-80) had 49 mice embryos onboard.…”

Section: Mammalsmentioning

confidence: 99%

Effects of microgravity on cell cytoskeleton and embryogenesis

Crawford-Young

2006

Int. J. Dev. Biol.

146

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The aim of this review is to compile, summarize and discuss the effects of microgravity on embryos, cell structure and function that have been demonstrated from data obtained during experiments performed in space or in altered gravity induced by clinostats. In cells and tissues cellular structure and genetic expression may be changed in microgravity and this has a variety of effects on embryogenesis which include death of the embryo, failure of neural tube closure, or final deformities to the overall morphology of the newborn or hatchling. Many species and protocols have been used for microgravity space experiments making it difficult to compare results. Experiments on the ways in which embryonic development and cell interactions occur in microgravity could also be performed. Experiments that have been done with cells in microgravity show changes in morphology, cytoskeleton and function. Changes in cytoskeleton have been noted and studies on microtubules in gravity have shown that they are gravity sensitive. Further study of basic chemical reactions that occur in cells should be done to shed some light on the underling processes leading to the changes that are observed in cells and embryos in microgravity.

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“…Additionally, the observed changes were associated with a change in the hormonal status of the females under the condition of weightlessness. When studying the early stages of development under the condition of weightlessness, mice were unable to become pregnant because pre-implanted embryos were aborted [24], possibly because of a change in the structure of the sex cells.…”

Section: Introductionmentioning

confidence: 99%

Cytoskeleton Structure in Mouse Sperm and Testes After 30 Days of Hindlimb Unloading and 12 Hours of Recovery

Usik,

Ogneva

2018

Cell Physiol Biochem

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Background/Aims: Changes in the external mechanical field result in cytoskeleton reorganization and the formation of adaptive patterns in different types of cells, including somatic cells and sex cells. The aim of this research was to study the protein and mRNA content of cytoskeletal and sperm-specific genes in the sperm and testis cells of mice. Methods: Mice were subjected to 30 days of antiorthostatic suspension to simulate weightlessness, followed by 12 h of recovery, while receiving essential phospholipids at a dosage of 500 mg/kg/day (30HSE and 30HSE+12h groups) or a similar dosage of a placebo (30HS and 30HS+12h groups). Accordingly, reference groups (CE group and C group) were formed. The total number and the percentage of motile spermatozoa were calculated using a Makler chamber. To analyze the number of viable spermatozoa and the permeability of their membranes, eosin staining was used as well as Diff-Quick for a morphological evaluation. Relative protein and mRNA content was estimated in a western blot and quantitative PCR assay, respectively. Results: The relative protein expression levels of actin (beta and gamma) and two alpha-actinin isoforms (1 and 4) remained constant in the sperm of all study groups, except for the 30HS+12h group, where the alpha-actinin-4 level was 13% higher than in the reference group (p < 0.1). In the testis cells, the relative actin isoform content was equivalent to that in the spermatozoa. However, in the testis cells, the ACTN1 mRNA content was 17% higher in the 30HS group than in the C group (p < 0.05), and decreased after 12 h of recovery. In contrast, the ACTN4 mRNA content was 20% lower in the 30HS group than in the reference group (p < 0.05) and increased after the 12-h recovery period. At the same time, in the group administered the essential phospholipids, the relative ACTN1 and ACTN4 mRNA content did not differ from those of the reference group. The relative beta-tubulin content was similar in the reference C group and the reference CE group, which was administered the essential phospholipids. In the 30HS and 30HS+12h groups, the beta-tubulin content decreased by 19% and 22% (p < 0.05), respectively, and they also decreased in the groups administered the essential phospholipids (30HSE and 30HSE+12h groups, by 27% and 33%, respectively, p < 0.05). In the testis tissue, the relative tubulin content did not change in any of the experimental groups. At the same time, the relative mRNA content of the genes encoding the studied cytoskeletal proteins increased, which may indicate the protein content was regulated mainly at the translational level. Conclusion: The spermogram parameters and the content of the sperm-specific proteins and the associated mRNAs revealed a decrease in the number of mature spermatozoa in mice suspended under conditions of weightlessness. Moreover, the decrease was prevented by the administration of essential phospholipids.

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Effects of simulated microgravity on mammalian fertilization and preimplantation embryonic development in vitro

Cited by 34 publications

References 14 publications

Real-Time Micrography of Mouse Preimplantation Embryos in an Orbit Module on SJ-8 Satellite

Real-Time Micrography of Mouse Preimplantation Embryos in an Orbit Module on SJ-8 Satellite

Effects of microgravity on cell cytoskeleton and embryogenesis

Cytoskeleton Structure in Mouse Sperm and Testes After 30 Days of Hindlimb Unloading and 12 Hours of Recovery

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