Decreasing bag limits is a management mechanism for enhancing size structure of Bluegill Lepomis macrochirus. However, restrictive bag limits can promote culling, where an angler returns a live fish to the water in exchange for another. Little is known about the effect of culling on ice-angled fishes.Our objective was to compare the effects of Bluegill confinement methods (reference, ice well, and bucket) and holding durations (0, 1, 2, or 5 hours) on changes in water quality parameters (dissolved oxygen, pH, carbon dioxide, and water temperature) and Bluegill stress physiology (blood glucose and plasma cortisol), reflex responses (RAMP scores), and mortality while ice-angling. In February 2018, 182 Bluegill were angled through the ice and randomly assigned a confinement method and holding duration. Bluegill blood glucose levels were higher in both confinement methods than reference fish at 2 and 5-hour holding durations. Bluegill had higher blood glucose levels in buckets than ice wells at 1 and 2 hours, but had higher blood glucose levels in ice wells at 5 hours. Water temperature was warmer in buckets than ice wells at all holding durations, while ice wells were cooler than ambient lake temperature. Bucket pH was higher than the lake at 2 hours and ice well pH was higher than the lake at 1 and 2 hours. Bluegill RAMP scores were similar across all holding durations and confinement methods, but were elevated in individuals held for the 24-hour mortality assessment.Two Bluegill mortalities occurred for fish held in ice wells. Our results suggest confinement method and holding duration while ice-angling can result in altered Bluegill blood glucose concentrations, water temperatures, and pH concentrations but that culling while ice-angling might not result in mortality. Consequently, culling practices may be compatible with and not negate the intended benefits of reduced Bluegill bag limits.Catch-and-release angling is a popular recreational activity driven by changes in angler conservation ethics and more restrictive harvest regulations (Cowx 2002;Cooke and Cowx 2004; Bartholmuw and Bohnsack 2005). Benefits of catch-and-release angling include enhanced recreational quality, increased catch rates, and improved size structure (Hubert and Quist 2010). However, success of catch-and-release practices relies on the assumption that released fish will survive after experiencing stressors associated with capture, handing, and confinement (Arlinghaus et al. 2007). The catch-and-
Accepted ArticleThis article is protected by copyright. All rights reserved release process can directly influence changes in fish stress physiology (e.g., changes in blood glucose and plasma cortisol concentrations) via handling practices (e.g., Pottinger 1998;Meka and McCormick 2005) and indirectly via changes associated with confinement water quality (e.g., water temperature, ammonia, carbon dioxide, and dissolved oxygen; Caldwell and Hinshaw 1994;Meka and McCormick 2005). Prolonged exposure to stressors associated with catch-and-release anglin...