Effects of silver sulphadiazine on the production of exoproteins by Staphylococcus aureus

Edwards-Jones, Valerie; Foster, H. A.

doi:10.1099/0022-1317-51-1-50

Cited by 6 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, an attempt was made to determine the pathogenicity of obtained Salmonella isolates by CR binding assay and hemolysin assays which yielded results in acceptance with [ 26 - 28 ]. Findings on the hemolytic strains of Salmonella observed is in agreement with a study of hemolysin pattern of Salmonella gallinarum in poultry reporting multiplicity in the hemolysin production among the pathogenic strain [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation, antibiogram and pathogenicity of Salmonella spp. Recovered from slaughtered food animals in Nagpur region of Central India

et al. 2016

View full text Add to dashboard Cite

Aim:To determine the prevalence, antibiogram and pathogenicity of Salmonella spp. in the common food animals slaughtered for consumption purpose at government approved slaughter houses located in and around Nagpur region during a period of 2010-2012.Materials and Methods:A total of 400 samples comprising 50 each of blood and meat from each slaughtered male cattle, buffaloes, pigs and goats were collected. Isolation was done by pre-enrichment in buffered peptone water and enrichment in Rappaport-Vassiliadis broth with subsequent selective plating onto xylose lysine deoxycholate agar. Presumptive Salmonella colonies were biochemically confirmed and analyzed for pathogenicity by hemolysin production and Congo red dye binding assay (CRDA). An antibiotic sensitivity test was performed to assess the antibiotic resistance pattern of the isolates.Results:A total of 10 isolates of Salmonella spp. from meat (3 from cattle, 1 from buffaloes and 6 from pigs) with an overall prevalence of 5% among food animals was recorded. No isolation was reported from any blood samples. Pathogenicity assays revealed 100% and 80% positivity for CRDA and hemolytic activity, respectively. Antimicrobial sensitivity test showed multi-drug resistance. The overall resistance of 50% was noted for trimethoprim followed by ampicillin (20%). A maximum sensitivity (80%) was reported to gentamycin followed by 40% each to ampicillin and trimethoprim, 30% to amikacin and 10% to kanamycin.Conclusion:The presence of multidrug resistant and potentially pathogenic Salmonella spp. in slaughtered food animals in Nagpur region can be a matter of concern for public health.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation, antibiogram and pathogenicity of Salmonella spp. Recovered from slaughtered food animals in Nagpur region of Central India

et al. 2016

View full text Add to dashboard Cite

show abstract

“…This mode of suppression has been reported to include the production of proteins such as ␣-and ␥-hemolysin, enterotoxins A and B, coagulase, and TSST-1 (5,13,17,20). These antibiotics are recommended for the treatment of severe staphylococcal illnesses associated with the secretion of these toxins.…”

Section: Discussionmentioning

confidence: 99%

Eugenol Reduces the Expression of Virulence-Related Exoproteins in Staphylococcus aureus

Qiu

Feng

Lü

et al. 2010

Appl Environ Microbiol

116

View full text Add to dashboard Cite

Eugenol, an essential oil component in plants, has been demonstrated to possess activity against both Gram-positive and Gram-negative bacteria. This study examined the influence that subinhibitory concentrations of eugenol may have on the expression of the major exotoxins produced by Staphylococcus aureus. The results from a tumor necrosis factor (TNF) release assay and a hemolysin assay indicated that S. aureus cultured with graded subinhibitory concentrations of eugenol (16 to 128 g/ml) dose dependently decreased the TNF-inducing and hemolytic activities of culture supernatants. Western blot analysis showed that eugenol significantly reduced the production of staphylococcal enterotoxin A (SEA), SEB, and toxic shock syndrome toxin 1 (the key exotoxins to induce TNF release), as well as the expression of ␣-hemolysin (the major hemolysin to cause hemolysis). In addition, this suppression was also evaluated at the transcriptional level via real-time reverse transcription (RT)-PCR analysis. The transcriptional analysis indicated that 128 g/ml of eugenol remarkably repressed the transcription of the S. aureus sea, seb, tst, and hla genes. According to these results, eugenol has the potential to be rationally applied on food products as a novel food antimicrobial agent both to inhibit the growth of bacteria and to suppress the production of exotoxins by S. aureus.Staphylococcus aureus is a major nosocomial pathogen. This species is capable of causing a wide range of diseases and is often associated with high morbidity and mortality rates. The disease spectrum includes food-borne illness, cutaneous infections, endocarditis, pneumonia, septic arthritis, and osteomyelitis (24, 36). The diversity is, in part, dependent on the secretion of a broad spectrum of soluble extracellular proteins. These proteins include enterotoxins, hemolysins, toxic shock syndrome toxin 1 (TSST-1), and others (20). S. aureus infections are difficult to control due to a combination of toxinmediated virulence, invasiveness, and antibiotic resistance. Therefore, there is a continuing need to discover new and improved antimicrobial agents to treat S. aureus illnesses, with potential benefits for both the food and pharmaceutical industries (35).Enterotoxins, TSST-1, and ␣-hemolysin are among the major exotoxins secreted by S. aureus. Staphylococcal enterotoxins (SEs) are a group of major serological heat-stable enterotoxins (SEA to SEE and SEG to SEJ) (3). These toxins cause staphylococcal gastroenteritis, food poisoning, toxic shock-like syndromes, and several allergic and autoimmune diseases (3,22,35). TSST-1 is the key causative toxin of toxic shock syndrome (TSS), which is an acute-onset and potentially lifethreatening staphylococcal syndrome (23). Furthermore, SEs and TSST-1 are also known to be pyrogenic toxin superantigens (PTSAgs) that stimulate the proliferation of T lymphocytes and the release of T-cell-derived cytokines (12). ␣-Hemolysin is a 33-kDa pore-forming soluble protein that has hemolytic, cytolytic, and dermonecrotic activities....

show abstract

“…The proteolytic activity analysis was performed as described by Edwards‐Jones & Foster (2002). In brief, 100 μL of the supernatant from postexponential‐phase (OD 600 nm of 2.5) cultures was added to 1 mL of azocasein (Sigma‐Aldrich) and incubated at 37 °C for 1 h. One millilitre of 5% w/v trichloroacetic acid was used to stop the reaction; undigested azocasein was allowed to precipitate for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Influence of subinhibitory concentrations of licochalcone A on the secretion of enterotoxins A and B by Staphylococcus aureus

Qiu

Feng

Xiang

et al. 2010

FEMS Microbiology Letters

View full text Add to dashboard Cite

Enterotoxins produced by Staphylococcus aureus are the key pathogenicity factors that can cause a variety of illnesses in humans, including staphylococcal gastroenteritis and food poisoning. It has been proven that licochalcone A is a potentially effective antimicrobial agent against S. aureus. In this study, Western blot assays, tumour necrosis factor release assays, murine T-cell proliferation assays, and real-time reverse transcriptase-PCR were performed to evaluate the effect of subinhibitory concentrations of licochalcone A on the secretion of two major enterotoxins (SEA and SEB) by S. aureus. The results show that licochalcone A significantly decreased, in a dose-dependent manner, the secretion of SEA and SEB by both methicillin-sensitive S. aureus and methicillin-resistant S. aureus. These results may increase the desirability of using licochalcone A as a lead compound for the design of more potent antibacterial agents based on the chalcone template.

show abstract

Effects of silver sulphadiazine on the production of exoproteins by Staphylococcus aureus

Cited by 6 publications

References 28 publications

Isolation, antibiogram and pathogenicity of Salmonella spp. Recovered from slaughtered food animals in Nagpur region of Central India

Isolation, antibiogram and pathogenicity of Salmonella spp. Recovered from slaughtered food animals in Nagpur region of Central India

Eugenol Reduces the Expression of Virulence-Related Exoproteins in Staphylococcus aureus

Influence of subinhibitory concentrations of licochalcone A on the secretion of enterotoxins A and B by Staphylococcus aureus

Contact Info

Product

Resources

About