Effects of silica on human lung fibroblasts: Survival data analysis of time-lapse cinematography data

Absher, Marlene; Sylwester, David

doi:10.1016/0013-9351(81)90219-x

Cited by 9 publications

(6 citation statements)

References 20 publications

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“…In conclusion, many minerals, particularly at low doses, appear to promote DNA levels in growing lung fibroblast cultures; whether or not this is related to initial elevated growth in the cultures has not been established in the present studies, but one mineral (a quartz) has been shown to enhance proliferation of human lung fibroblasts in vitro (11). A direct relationship between DNA and hydroxyproline levels in fibroblast cultures exposed to minerals for up to 3 weeks in vitro is obtained with some dusts (DQ 12 quartz, A380 silica and activated charcoal).…”

Section: Mineralsmentioning

confidence: 83%

Reaction of mineral dusts with primary lung fibroblast cultures.

Richards¹,

Hunt²

1983

Environ Health Perspect

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The complex metabolism of rabbit lung fibroblast cultures maintained over 24 days in vitro and their interaction with the fibrogenic group of asbestos minerals is reviewed. The differential effects of nine dusts given as a single dose to 3-day-old fibroblast cultures which were then analyzed for DNA, protein and cell mat hydroxyproline after 3 weeks are discussed.A comparison is made of the ability of these minerals to promote reticulin deposition in vivo following instillation into rats with the ability of each mineral to alter cell mat hydroxyproline levels in fibroblast cultures. Because this comparison is poor for some of the minerals investigated, it is concluded that with the current methodology the fibroblast cultures do not provide an adequate in vitro test system for assessing the potential fibrogenicity of a mineral in vivo.

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Section: Mineralsmentioning

confidence: 83%

Reaction of mineral dusts with primary lung fibroblast cultures.

Richards¹,

Hunt²

1983

Environ Health Perspect

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“…With one mineral (DQ12 quartz) there is a direct relationship between increase in DNA levels and dose. Min-u-sil quartz has also been shown to raise DNA levels in fibroblast cultures (49), and other investigators have shown that quartz will enhance the proliferation of human lung fibroblasts in vitro (53). Therefore if it is assumed that more cells are present in quartz-treated cultures, then they should ultimately deposit more collagen; indeed, this seems to be the case (Fig.…”

Section: It Is Important To State That the Hydroxyproline-containinmentioning

confidence: 95%

Biochemical and Cellular Mechanisms of Dust-Induced Lung Fibrosis

Richards¹,

Curtis²

1984

Environmental Health Perspectives

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“…If however the cell stays in G0 longer than a certain period of time it changes to a differentiated HDF and these D-HDFs are modelled as having no stimulatory or inhibitory influence on NHKs. The intrinsic cell cycle designated to HDF is 20 hours (40 iterations) based on literature values [70]–[72].…”

Section: Introductionmentioning

confidence: 99%

“…Accordingly a cell passive movement was applied to each agent corresponding to the forces acting on it. An active migration speed of approximately 1 µm/minute was also applied to HDF based on time lapse experiments and literature [70]. (Summarised in Table 3).…”

Section: Introductionmentioning

confidence: 99%

Agent Based Modelling Helps in Understanding the Rules by Which Fibroblasts Support Keratinocyte Colony Formation

et al. 2008

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BackgroundAutologous keratincoytes are routinely expanded using irradiated mouse fibroblasts and bovine serum for clinical use. With growing concerns about the safety of these xenobiotic materials, it is desirable to culture keratinocytes in media without animal derived products. An improved understanding of epithelial/mesenchymal interactions could assist in this.Methodology/Principal FindingsA keratincyte/fibroblast o-culture model was developed by extending an agent-based keratinocyte colony formation model to include the response of keratinocytes to both fibroblasts and serum. The model was validated by comparison of the in virtuo and in vitro multicellular behaviour of keratinocytes and fibroblasts in single and co-culture in Greens medium. To test the robustness of the model, several properties of the fibroblasts were changed to investigate their influence on the multicellular morphogenesis of keratinocyes and fibroblasts. The model was then used to generate hypotheses to explore the interactions of both proliferative and growth arrested fibroblasts with keratinocytes. The key predictions arising from the model which were confirmed by in vitro experiments were that 1) the ratio of fibroblasts to keratinocytes would critically influence keratinocyte colony expansion, 2) this ratio needed to be optimum at the beginning of the co-culture, 3) proliferative fibroblasts would be more effective than irradiated cells in expanding keratinocytes and 4) in the presence of an adequate number of fibroblasts, keratinocyte expansion would be independent of serum.ConclusionsA closely associated computational and biological approach is a powerful tool for understanding complex biological systems such as the interactions between keratinocytes and fibroblasts. The key outcome of this study is the finding that the early addition of a critical ratio of proliferative fibroblasts can give rapid keratinocyte expansion without the use of irradiated mouse fibroblasts and bovine serum.

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Effects of silica on human lung fibroblasts: Survival data analysis of time-lapse cinematography data

Cited by 9 publications

References 20 publications

Reaction of mineral dusts with primary lung fibroblast cultures.

Reaction of mineral dusts with primary lung fibroblast cultures.

Biochemical and Cellular Mechanisms of Dust-Induced Lung Fibrosis

Agent Based Modelling Helps in Understanding the Rules by Which Fibroblasts Support Keratinocyte Colony Formation

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