Effects of Sildenafil Citrate, Isoniazid, and Streptomycin on Testicular Tissue and Epididymal Semen Quality in Rats

Alp, Harun; Cirit, Ümüt; Taş, Muzaffer; Rifaioğlu, Murat Mehmet; Hatipoğlu, Namık Kemal; Aytekin, İsmail; Yücel, Mehmet; Fırat, Uğur; Özmen, Mehmet Ferit; Şeker, Uğur; Eren, Leyla Bilge

doi:10.1016/j.urology.2012.05.016

Cited by 20 publications

(12 citation statements)

References 21 publications

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“…In contrast to previous studies that tested similar antibiotics and duration of treatment (Moe et al 1989, Crotty et al 1995, Demir et al 2007, Aral et al 2008, Farombi et al 2008, El-Harouny et al 2010, Antohi et al 2011, Alp et al 2012, Elzeinova et al 2013, Ojo et al 2013, Silla et al 2015), no effects on spermatogenesis were evident in the current studies. Careful examination of prior reports of antibiotic-induced testicular effects (Aral et al 2008, Farombi et al 2008, Elzeinova et al 2013, Ojo et al 2013), however, revealed critical concerns in the histopathological interpretation that might be attributed to usage of methods that are sub-optimal for testicular toxicological studies.…”

Section: Discussioncontrasting

confidence: 99%

“…While past studies have not considered the potential effects of antibiotic treatment on the resident seminal vesicle microbiome, the effects of several bacteriostatic and bactericidal antibiotics on the testes and epididymides have been examined. Short-term antibiotic treatment of rodent models and amphibians with antibiotics, including tetracycline, doxycycline, gentamycin, ciprofloxacin, enrofloxacin, ofloxacin, salinomycin, streptomycin, ceftazidime and cefmetazole, results in impaired spermatogenesis, decreased sperm motility, suppression of androgen production by Leydig cells and generation of oxygen-free radicals within the male reproductive tract (Moe et al 1989, Crotty et al 1995, Demir et al 2007, Aral et al 2008, Farombi et al 2008, El-Harouny et al 2010, Antohi et al 2011, Alp et al 2012, Elzeinova et al 2013, Ojo et al 2013, Silla et al 2015). Some of these changes are reversible after cessation of the antibiotic treatment (Moe et al 1989, Ojo et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Seminal fluid metabolome and epididymal changes after antibiotic treatment in mice

Rosenfeld¹,

Javurek²,

Johnson³

et al. 2018

Reproduction

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Paternal environment can induce detrimental developmental origins of health and disease (DOHaD) effects in resulting offspring and even future descendants. Such paternal-induced DOHaD effects might originate from alterations in a possible seminal fluid microbiome (SFM) and composite metabolome. Seminal vesicles secrete a slightly basic product enriched with fructose and other carbohydrates, providing an ideal habitat for microorganisms. Past studies confirm the existence of a SFM that is influenced by genetic and nutritional status. Herein, we sought to determine whether treatment of male mice with a combination of antibiotics designed to target SFM induces metabolic alterations in seminal vesicle gland secretions (seminal fluid) and histopathological changes in testes and epididymides. Adult (10- to 12-week-old) National Institutes of Health (NIH) Swiss males ( = 10 per group) were treated with Clindamycin 0.06 mg/kg day, Unasyn (ampicillin/sulbactam) 40 mg/kg day and Baytril (enrofloxacin) 50 mg/kg day designed to target the primary bacteria within the SFM or saline vehicle alone. Fourteen-day antibiotic treatment of males induced metabolomic changes in seminal vesicles with inosine, xanthine and l-glutamic acid decreased but d-fructose increased in glandular secretions. While spermatogenesis was not affected in treated males, increased number of epididymal tubules showed cribriform growth in this group (7 antibiotic-treated males: 3 saline control males; = 0.01). Antibiotic-treated males showed more severe cribriform cysts. Current findings suggest antibiotic treatment of male mice results in seminal fluid metabolome and epididymal histopathological alterations. It remains to be determined whether such changes compromise male reproductive function or lead to DOHaD effects in resulting offspring.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Seminal fluid metabolome and epididymal changes after antibiotic treatment in mice

Rosenfeld¹,

Javurek²,

Johnson³

et al. 2018

Reproduction

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“…This is likely the reason for the decreased sperm count observed in TRA and TRA + VIT E as the concentration of testosterone was insufficient to facilitate spermatogenesis. This finding contradicts the report of Alp et al () but corroborates that of Osadolor and Omo‐Erhabor () and Nna et al () which showed decreased serum testosterone, LH and FSH levels following tramadol administration.…”

Section: Discussioncontrasting

confidence: 72%

Vitamin E administration does not ameliorate tramadol‐associated impairment of testicular function in Wistar rats

et al. 2019

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Tramadol is widely abused in Nigeria and has been reported to cause fertility decline via testicular oxidative stress. This study investigated the effect of vitamin E, an antioxidant on some reproductive parameters in male Wistar rats administered tramadol. Twenty male Wistar rats (180-200 g) were randomly assigned into four groups (n = 5) thus: Control (0.2 ml vehicle: olive oil), tramadol-treated (20 mg/kg of tramadol), vitamin E-treated (100 mg/kg of vitamin E) and tramadol + vitamin Etreated (received tramadol and vitamin E) groups. Drugs were administered orally and daily for 28 days. Sperm count, Johnsen's score, germinal epithelial height and serum testosterone, follicle-stimulating hormone (FSH) and luteinising hormone (LH) concentrations were significantly (p < .05) decreased in tramadol-treated and tramadol + vitamin E compared with control and vitamin E-treated groups. Sperm motility, morphology, viability, seminiferous tubular diameter, Leydig cell count, Sertoli cell count and malondialdehyde, superoxide dismutase, glutathione peroxidase and catalase concentrations were not significantly different among the groups. Histology of testis and epididymis in all groups showed no toxicity but decreased sperm population in tramadol-treated and tramadol + vitamin E-treated groups. Tramadol did not cause testicular oxidative stress but impaired testicular function by suppressing testosterone, FSH and LH secretion. Vitamin E administration could not attenuate this impairment in testicular function. K E Y W O R D S spermatogenesis, testis, testosterone, tramadol, vitamin E How to cite this article: Udefa AL, Beshel FN, Nwangwa JN, et al. Vitamin E administration does not ameliorate tramadolassociated impairment of testicular function in Wistar rats.

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“…As for testicular toxicity this problem remains unclear. To date there is single record on decreased testicular biopsy score in INH-treated laboratory animals (Alp et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Induction of CYP2E1 in testes of isoniazid-treated rats as possible cause of testicular disorders

et al. 2015

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Effects of Sildenafil Citrate, Isoniazid, and Streptomycin on Testicular Tissue and Epididymal Semen Quality in Rats

Cited by 20 publications

References 21 publications

Seminal fluid metabolome and epididymal changes after antibiotic treatment in mice

Seminal fluid metabolome and epididymal changes after antibiotic treatment in mice

Vitamin E administration does not ameliorate tramadol‐associated impairment of testicular function in Wistar rats

Induction of CYP2E1 in testes of isoniazid-treated rats as possible cause of testicular disorders

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