Effects of short‐term storage on sperm function in fish semen: a review

Contreras, Pablo; Ulloa‐Rodríguez, Patricio; Merino, O.; Figueroa, Elías; Farías, Jorge G.; Valdebenito, Iván; Risopatrón, Jennie

doi:10.1111/raq.12387

Cited by 59 publications

(47 citation statements)

References 106 publications

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“…Most authors report an improvement in samples storability by the use of an extender solution (Gallego et al 2013;Santos et al 2018;González-López et al 2019). The use of these extender solutions is beneficial for several reasons such as reduces the sperm density and thus improves the oxygen supply, controls pH through the use of buffers such as Tris in our case, prevents the dehydration of the cells and decreases the harmful effect of urine contamination (reviewed by Contreras et al 2019;Beirão et al 2019). However, in most cases reported the extender resembles the seminal plasma and is designed to keep the sperm in quiescent immotile state until activation by contact with fresh (freshwater species) or seawater (marine species) (Contreras et al 2019), which is not the case of spotted wolffish.…”

Section: Discussionmentioning

confidence: 96%

“…The use of these extender solutions is beneficial for several reasons such as reduces the sperm density and thus improves the oxygen supply, controls pH through the use of buffers such as Tris in our case, prevents the dehydration of the cells and decreases the harmful effect of urine contamination (reviewed by Contreras et al 2019;Beirão et al 2019). However, in most cases reported the extender resembles the seminal plasma and is designed to keep the sperm in quiescent immotile state until activation by contact with fresh (freshwater species) or seawater (marine species) (Contreras et al 2019), which is not the case of spotted wolffish. Few exceptions exist for internal fertilizing species, such as guppy Poecilia reticulata (Sun et al 2010), where the sperm was continuously motile in the extender solution.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, cryopreservation procedures are often used for long-term sperm storage (Cabrita et al 2010). Shortterm storage of sperm by refrigeration, on the other hand, is a simple and inexpensive procedure relatively easy to implement in the hatchery environment (Bobe and Labbé 2008;Contreras et al 2019). Sperm refrigeration protocols have been tested in several species and can be a useful tool to assist in artificial fertilization programmes, synchronization in availability of gametes and transport of gametes between different facilities (Contreras et al 2019;Beirão et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Is it possible to store spotted wolffish (Anarhichas minor) sperm by refrigeration?

González-López

Patel

Duncan

et al. 2020

Fish Physiol Biochem

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Spotted wolffish Anarhichas minor reproduction in captivity is dependent on in vitro fertilization. However, it is often challenging to acquire sufficient fresh sperm to fertilize the eggs that are obtained. In this study, we evaluate the possibility to store spotted wolffish sperm by refrigeration. Spotted wolffish sperm has the particularity that is already motile on stripping, and currently it is not possible to immobilize and reactivate. Thus, sperm refrigeration protocols should focus in extending this motility period that usually lasts up to 2 days. In a first experiment, we evaluated the possibility that the motility period of the sperm was limited by contamination with urine. The urea concentration in the sperm obtained both by stripping (17.10 ± 1.98 mg/dL) and directly from the testis (12.59 ± 2.37 mg/dL) was similar (p > 0.05), which indicate that the sperm collection method used avoid contamination with urine. Afterwards, we tested the possibility that the sperm motility period was limited by energy stores. The ATP concentration (initial value 5.65 ± 0.86 nmol/10 9 cells) remained stable (p = 0.099) during 30 h after sperm collection, and similar values (p = 0.329) were recorded at end of sperm storage in both diluted (3.88 ± 1.35 nmol/10 9 cells) and undiluted samples (4.76 ± 1.08 nmol/10 9 ). This indicates that the low intracellular ATP consumption, derived from the slow sperm motility, can probably be compensated rapidly enough by mitochondrial synthesis of ATP in the spotted wolffish sperm. In both experiments, diluted sperm kept higher percentage of motile cells during the storage time.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Is it possible to store spotted wolffish (Anarhichas minor) sperm by refrigeration?

González-López

Patel

Duncan

et al. 2020

Fish Physiol Biochem

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“…In aquaculture, semen storage is important for the conservation of gametes. Semen can be stored at low temperatures for a short period of time, from a few hours to a few days [ 117 , 118 ]. The main aim of short-term storage is the improvement of reproductive procedures and preservation of semen under adequate conditions to maintain its fertilising capacity.…”

Section: Short-term Semen Storagementioning

confidence: 99%

Characteristics and Cryopreservation of Semen of Sex-Reversed Females of Salmonid Fish

Judycka

Nynca

Hliwa

et al. 2021

IJMS

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Sex reversal has been used as a breeding strategy by salmonid fish to produce genetically and phenotypically single sex populations. Production of all-female fish has great importance for the creation of monosex female triploids of salmonid fish, which are valued for their sterility, lack of female maturation, and larger commercial size. Among salmonids, the majority of rainbow trout (Oncorhynchus mykiss) production is based on all-female production with a high proportion of all-female triploid production in Europe. The main aim of this review is to present the recent knowledge regarding sex-reversed females (SRFs) of salmonid fish. We discuss the methods of sex reversal as well as their effects on the morphology and histology of the reproductive tract. We focus on the characteristics of SRF semen as well as the factors determining semen quality. The lower quality of SRF sperm compared to that of normal males has resulted in the need for the artificial maturation of semen. Most importantly, methods of semen storage—both short-term and long-term (cryopreservation)—that can improve hatchery operations are presented with the special emphasis on recent progress in development of efficient cryopreservation procedures and use of cryopreserved semen in hatchery practice. Moreover, we also address the emerging knowledge concerning the proteomic investigations of salmonid sperm, focusing primarily on the proteomic comparison of normal male and SRF testicular semen and presenting changes in SRF rainbow trout sperm proteome after in vitro incubation in artificial seminal plasma.

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“…During in vitro storage of fish sperm, its quality can reduce significantly (motility, viability, integrity of plasma membrane, mitochondrial membrane potential, Contreras et al 2017;Risopatrón et al 2018;Trigo et al 2015). Sperm storage time (hours to days) highly depend on the appropriate method and on the species (Beirão et al 2019;Contreras et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Standardization of sperm management for laboratory assessment of sperm quality and in vitro fertilization in Eurasian perch (Perca fluviatilis)

et al. 2021

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Sperm handling procedures and its usage for in vitro fertilization are crucial for standardized experimental operations on evaluation of reproductive performance, gamete quality, and optimization of fertilization protocols. In our study, the changes in perch sperm motility parameters within 6-h chilled storage and using 4 different activating solutions were compared. Eight different sperm-to-egg ratio was also compared during fertilization. Sperm activated with modified activating solution for cyprinids (78±11%), common perch activating solution (68±16%), modified Lahnsteiner activating solution (75±16%), and Woynárovich solution (76±13%) showed similar progressive motility at 10 s after activation. At 30 s after activation, progressive motility decreased below 5%, regardless the activating solution used. Progressive motility decreased significantly already after 2 h of storage (51±19%) in comparison with 0 h (78±5%). The highest average fertilization rate (using common perch activating solution) was observed with a sperm-to-egg ratio 2.5×105:1 (80±9%), where the smallest variability in the values was also recorded (coefficient of variation: 11%). However, no significant difference was detected among the 8 sperm-to-egg ratio groups. According to our findings, undiluted fresh perch sperm is recommended to use in 1 h post-stripping. Modified Lahnsteiner’s activating solution can be applied efficiently for quality assessment where common perch activating solution is applicable for fertilization in Eurasian perch. A sperm-to-egg ratio 2.5×105:1 already allows to achieve a high fertilization rate; however, the finding is needed to be tested also at hatchery level (higher number of eggs).

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Effects of short‐term storage on sperm function in fish semen: a review

Cited by 59 publications

References 106 publications

Is it possible to store spotted wolffish (Anarhichas minor) sperm by refrigeration?

Is it possible to store spotted wolffish (Anarhichas minor) sperm by refrigeration?

Characteristics and Cryopreservation of Semen of Sex-Reversed Females of Salmonid Fish

Standardization of sperm management for laboratory assessment of sperm quality and in vitro fertilization in Eurasian perch (Perca fluviatilis)

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