Effects of short-term exposure of paracetamol in the gonads of blue mussels Mytilus edulis

Koagouw, Wulan; Ciocan, Corina

doi:10.1007/s11356-019-06861-w

Cited by 17 publications

(32 citation statements)

References 77 publications

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“…This study explores the impacts of paracetamol on the gonads of marine mussels Mytilus edulis after a long-term exposure, and discusses the potential reproductive challenges that may arise. Here, we present our data alongside and in comparison with results from a previous short-term exposure (Koagouw and Ciocan, 2019), in order to demonstrate the importance of the length of exposure on the potential biological and ecological damage in icted by paracetamol. Mussels are excellent indicator organisms for environmental monitoring and have been intensively used worldwide to monitor marine pollution (Rittschof and McClellan-Green, 2005).…”

Section: Introductionmentioning

confidence: 94%

“…The detailed protocol is outlined in Koagouw & Ciocan (2019). Water samples (1000 mL) were obtained from each group 15-30 minutes after paracetamol was added (t 0 ) and immediately before arti cial seawater was changed (72 and 96 hours).…”

Section: Water Analysismentioning

confidence: 99%

“…The area below the peak was de ned by the reconstructed ion chromatogram of the fragment at 110.0602 m/z and quantitation was determined using Quan Browser data processing software (Xcalibur V:4.1.31.9, Thermo Scienti c). The detailed parameters of this procedure are described in Koagouw & Ciocan (2019).…”

Section: Water Analysismentioning

confidence: 99%

“…Histological examination was used both for the purposes of assessing the sex of individuals and for analysing any pathological conditions found in mussel gonads. The analysis was performed according to Koagouw & Ciocan (2019). The 7 µm slices cut from para n embedded blocks were stained with hematoxylin and eosin.…”

Section: Tissue Preparation For Histological Examinationmentioning

confidence: 99%

“…Molecular analysis was performed to investigate potential changes in the pattern of expression of selected transcripts, as described by Koagouw & Ciocan (2019): vitellogenin (VTG), vitelline envelope zona pellucida domain-9 (V9), estrogen receptor-2 (ER2), heat shock protein-70 (HSP70), caspase-8 (CASP8), B-cell lymphoma-2 (BCL2) and Fas cell surface death receptor (FAS). The same primers as in Koagouw & Ciocan (2019) were used for this study (Table S1).…”

Section: Quantitative Real Time Pcrmentioning

confidence: 99%

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Long term exposure of marine mussels to paracetamol: is time a healer or a killer?

Koagouw

Stewart

Ciocan

2021

Preprint

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Pharmaceuticals pose a major threat to the marine environment and several studies have recently described their negative effects on marine organisms. Pharmaceutical compounds are constantly being released into aquatic ecosystems and chronic exposure, even at low concentrations, may have impact on marine organisms. The purpose of the present study is to evaluate the biological changes induced by one of the most widely used pharmaceuticals – paracetamol – in the blue mussel Mytilus edulis, after a long-term exposure at environmentally relevant concentrations. We present our data alongside and in comparison with results from a previous short-term exposure, to demonstrate the significance of exposure period on the effects of paracetamol in adult blue mussels. After 24 days of laboratory exposure, seven potential target genes were selected to examine toxicological effects in mussels’ gonads and possible disruptive effects on reproductive processes. The results show the modulation of some important reproduction-related genes: estrogen receptor-2 (ER2), vitelline envelope zona pellucida domain-9 (V9) and vitellogenin (VTG). Variations in mRNA expression of four other genes involved in apoptosis (HSP70, CASP8, BCL2 and FAS) are also highlighted. Histopathological alterations caused by paracetamol, together with neutral red retention time response in mussels’ hemocytes, are presented herein. Overall, this study highlights the exacerbated effects of low concentration of paracetamol after chronic exposure, similar to the damage induced by higher concentrations in a short exposure scenario, thus emphasizing the importance of length of exposure period when studying the effects of this substance. Additionally, this study also discusses the potential of paracetamol to inflict several major changes on the reproductive system of mussels and thus possibly affect the survival of populations.

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Section: Introductionmentioning

confidence: 94%

Section: Water Analysismentioning

confidence: 99%

Section: Water Analysismentioning

confidence: 99%

Section: Tissue Preparation For Histological Examinationmentioning

confidence: 99%

Section: Quantitative Real Time Pcrmentioning

confidence: 99%

See 3 more Smart Citations

Long term exposure of marine mussels to paracetamol: is time a healer or a killer?

Koagouw

Stewart

Ciocan

2021

Preprint

Self Cite

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show abstract

Special issue on challenges in emerging environmental contaminants CEEC19

Libralato

Freitas

Buttino

et al. 2020

Environ Sci Pollut Res

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Long-term exposure of marine mussels to paracetamol: is time a healer or a killer?

Koagouw

Stewart

Ciocan

2021

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

Pharmaceuticals pose a major threat to the marine environment, and several studies have recently described their negative effects on marine organisms. Pharmaceutical compounds are constantly being released into aquatic ecosystems, and chronic exposure, even at low concentrations, may have a major impact on marine organisms. The purpose of the present study is to evaluate the biological changes induced by one of the most widely used pharmaceuticals-paracetamol-in the blue mussel Mytilus edulis, after a long-term exposure at environmentally relevant concentrations. We present our data alongside and in comparison with results from a previous short-term exposure, to demonstrate the significance of exposure period on the effects of paracetamol in adult blue mussels. After 24 days of laboratory exposure, seven potential target genes were selected to examine toxicological effects in mussels' gonads and possible disruptive effects on reproductive processes. The results show the modulation of some important reproduction-related genes: estrogen receptor-2 (ER2), vitelline envelope zona pellucida domain-9 (V9), and vitellogenin (VTG). Variations in mRNA expression of four other genes involved in apoptosis (HSP70, CASP8, BCL2, and FAS) are also highlighted. Histopathological alterations caused by paracetamol, together with neutral red retention time response in mussels' hemocytes, are presented herein. Overall, this study highlights the exacerbated effects of low concentration of paracetamol after chronic exposure, similar to the damage induced by higher concentrations in a short exposure scenario, thus emphasizing the importance of length of exposure period when studying the effects of this substance. Additionally, this study also discusses the potential of paracetamol to inflict several major changes in the reproductive system of mussels and thus possibly affect the survival of populations.

show abstract

Effects of short-term exposure of paracetamol in the gonads of blue mussels Mytilus edulis

Cited by 17 publications

References 77 publications

Long term exposure of marine mussels to paracetamol: is time a healer or a killer?

Long term exposure of marine mussels to paracetamol: is time a healer or a killer?

Special issue on challenges in emerging environmental contaminants CEEC19

Long-term exposure of marine mussels to paracetamol: is time a healer or a killer?

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