Effects of short chain fatty acids on mucosal proliferation and inflammation of ileal pouches in patients with ulcerative colitis and familial polyposis

Tonelli, Francesco; Dolara, Piero; Batignani, Giacomo; Monaci, Iacopo; Caderni, Giovanna; Spagnesi, M.; Luceri, Cristina; Amorosi, Andrea

doi:10.1007/bf02049735

Cited by 22 publications

(16 citation statements)

References 12 publications

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“…: POUCHITIS AFTER ILEAL POUCH-ANAL ANASTOMOSIS Various potential factors have been investigated to identify the etiology of CP, including bacterial overgrowth, mucosal atrophy and breakdown resulting from pouch ischemia, and pouchitis as a recurrence of UC. 9,[13][14][15][16] In our study, postoperative complications, specifically anastomotic complications, were associated with the development of pouchitis. This, in combination with the trend toward the decreased development of CP in patients not undergoing a diverting ileostomy, may indicate that the loss of gut integrity is important in the development of CP.…”

Section: Discussionmentioning

confidence: 89%

Predictors of Pouchitis after Ileal Pouch-Anal Anastomosis: A Retrospective Review

Hoda¹,

Collins²,

Knigge³

et al. 2008

Diseases of the Colon &Amp; Rectum

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Chronic pouchitis is a frequent complication after ileal pouch-anal anastomosis. In this study patients with primary sclerosing cholangitis or other extraintestinal manifestations of ulcerative colitis were not more likely to develop chronic pouchitis. Patients with postoperative complications, specifically anastomotic complications after ileal pouch-anal anastomosis, were more likely to develop chronic pouchitis and may benefit from early strategies to prevent pouchitis.

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Section: Discussionmentioning

confidence: 89%

Predictors of Pouchitis after Ileal Pouch-Anal Anastomosis: A Retrospective Review

Hoda¹,

Collins²,

Knigge³

et al. 2008

Diseases of the Colon &Amp; Rectum

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“…NaBu is emerging as an anti-inflammatory drug against ulcerative colitis (Breuer et al, 1991;Scheppach et al, 1992;Tonelli et al, 1995;Steinhart et al, 1996Steinhart et al, , 1994Vernia et al, 2003), diversion colitis (Harig et al, 1989), radiation proctitis (Vernia et al, 2000), pouchitis (Sandborn et al, 2006), and Crohn's disease (Di Sabatino et al, 2005). Our results suggest that NaBu is not able to counteract the effects of cytokines if the cells are already "bathed" with cytokines, implying that to prevent/cure inflammation, NaBu treatment should be started at a very early stage in the inflammatory process.…”

Section: Il-1␤ and Tnf-␣ Inhibit Iap Expression 691mentioning

confidence: 98%

The Pro-inflammatory Cytokines, IL-1βand TNF-α, Inhibit Intestinal Alkaline Phosphatase Gene Expression

Malo

Biswas

Abedrapo

et al. 2006

DNA and Cell Biology

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High levels of the pro-inflammatory cytokines, interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha), are present in the gut mucosa of patients suffering form various diseases, most notably inflammatory bowel diseases (IBD). Since the inflammatory milieu can cause important alterations in epithelial cell function, we examined the cytokine effects on the expression of the enterocyte differentiation marker, intestinal alkaline phosphatase (IAP), a protein that detoxifies bacterial lipopolysaccharides (LPS) and limits fat absorption. Sodium butyrate (NaBu), a short-chain fatty acid and histone deacetylase (HDAC) inhibitor, was used to induce IAP expression in HT-29 cells and the cells were also treated +/- the cytokines. Northern blots confirmed IAP induction by NaBu, however, pretreatment (6 h) with either cytokine showed a dose-dependent inhibition of IAP expression. IAP Western analyses and alkaline phosphatase enzyme assays corroborated the Northern data and confirmed that the cytokines inhibit IAP induction. Transient transfections with a reporter plasmid carrying the human IAP promoter showed significant inhibition of NaBu-induced IAP gene activation by the cytokines (100 and 60% inhibition with IL-1beta and TNF-alpha, respectively). Western analyses showed that NaBu induced H4 and H3 histone acetylation, and pretreatment with IL-1beta or TNF-alpha did not change this global acetylation pattern. In contrast, chromatin immunoprecipitation showed that local histone acetylation of the IAP promoter region was specifically inhibited by either cytokine. We conclude that IL-1beta and TNF-alpha inhibit NaBu-induced IAP gene expression, likely by blocking the histone acetylation within its promoter. Cytokine-mediated IAP gene silencing may have important implications for gut epithelial function in the setting of intestinal inflammatory conditions.

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“…Human studies analysing the effects of SCFAs were performed in patients with colonic inflammation using rectal enemas containing SCFA mixtures or butyrate alone (Table 1). 45, 46, 73, 96, 113–126 …”

Section: Butyrate and Inflammationmentioning

confidence: 99%

“…The effects of butyrate containing enemas on radiation proctitis 113, 117, 122, 125 and pouchitis 123 have been studied in small groups and besides one report 125 that showed that butyrate was an effective treatment of radiation proctitis, other studies did not report clear‐cut beneficial effects of SCFA irrigation in these two patient groups 113, 117, 122, 123 …”

Section: Butyrate and Inflammationmentioning

confidence: 99%

Review article: the role of butyrate on colonic function

Hamer¹,

Jonkers²,

Venema³

et al. 2007

Aliment Pharmacol Ther

2,224

1,612

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Effects of short chain fatty acids on mucosal proliferation and inflammation of ileal pouches in patients with ulcerative colitis and familial polyposis

Abstract: SCFA do not control inflammation and clinical functions but reduce cell proliferation in UC patients. On the contrary, FAP patients are refractory to SCFA.

Cited by 22 publications

References 12 publications

Predictors of Pouchitis after Ileal Pouch-Anal Anastomosis: A Retrospective Review

Predictors of Pouchitis after Ileal Pouch-Anal Anastomosis: A Retrospective Review

The Pro-inflammatory Cytokines, IL-1βand TNF-α, Inhibit Intestinal Alkaline Phosphatase Gene Expression

Review article: the role of butyrate on colonic function

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