Effects of shoot-applied gibberellin/gibberellin-biosynthesis inhibitors on root growth and expression of gibberellin biosynthesis genes in Arabidopsis thaliana

Bidadi, Haniyeh; Yamaguchi, Shinjiro; Asahina, Masashi; Satoh, Shinobu

doi:10.3117/plantroot.4.4

Cited by 36 publications

(30 citation statements)

References 32 publications

(27 reference statements)

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“…Other studies revealed that, early paclobutrazol application could clearly reduce the stem length in potato, accompanied with a significant increase in the number of tubers per plant, as well as the reduction in plant height and modification in the aboveground structure (Rodriguez-Falcon et al, 2006;Mariana and Hamdani, 2016). However, in this study, we showed that, the application of paclobutrazol has significant influence on increasing taproot weight and diameter, this outcome is supported by the findings of Bidadi et al (2010) in Arabidopsis, Wang et al (2015) in carrot and Mariana and Hamdani (2016) in potato. In contrast, our result showed that, GA3 treatment revealed a clear increase in shoot weight (Fig.…”

Section: Discussioncontrasting

confidence: 49%

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Effects of Gibberellin and Gibberellin Biosynthesis Inhibitor (Paclobutrazol) Applications on Radish (Raphanus sativus) Taproot Expansion and the Presence of Authentic Hormones

Jabir¹,

Kinuthia²,

Faroug³

et al. 2017

IJAB

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To cite this paper: Jabir, B. AbstractRadish (Raphanus sativus L), is a taproot plant that undergoes noticeable organ size modification during plant growth and development. Paclobutrazol (PBZ) inhibits gibberellin biosynthesis and stimulates the proliferation and expansion of plant root cells in growth and developmental stages. Our study aims to examine the effects of PBZ treatment on the storage organ of plant. Four treatments were applied at the pre-cortex splitting stage of "Nau-yh" radish plant. Foliar spray treatments including PBZ (50 mg/L), gibberellic acid (GA3, 150 mg/L) and a mix of GA3+PBZ (150+50 mg/L) were applied. The results revealed considerable improvement in taproot growth and decreases development in aerial parts after 48 days of the growth. Paclobutrazol treatment showed enhanced growth parameters, most notably, root weight was 33% higher than GA 3 treatment, 28% higher than the control (H2O), and 23% higher than the PBZ+GA 3 treatment. PBZ also significantly promoted xylem development in tuberous root of radish. In contrast, the GA3 treatment inhibited tuberous root growth and enhanced shoot growth in plants. Furthermore, mass spectrometry assay confirmed the absence of gibberellins in paclobutrazol-treated plants, whereas auxin was found in all treatments. Our study provides the first evidence on the applicability of PBZ in root size expansion of radish plant.

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Section: Discussioncontrasting

confidence: 49%

“…The variation in xylem, cortex and vessel cells in radish root were associated with changes, which lead to increase the thickness of the radish taproot. In general, root thickening occurred by a combination of cell division and cell enlargement (Bidadi et al, 2010;Guo, et al, 2015;Wang et al, 2015;Mabvongwe et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Effects of Gibberellin and Gibberellin Biosynthesis Inhibitor (Paclobutrazol) Applications on Radish (Raphanus sativus) Taproot Expansion and the Presence of Authentic Hormones

Jabir¹,

Kinuthia²,

Faroug³

et al. 2017

IJAB

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“…Conversely, depletion of endogenous GA levels using the GA biosynthesis inhibitor uniconazole (Izumi et al, 1984) promoted disease susceptibility. Although care should be taken when interpreting these results since the specificity of uniconazole is not entirely clear (Bidadi et al, 2010), these data strongly suggest that GA acts as a positive player in rice immunity to P. graminicola (Supplemental Fig. S5B).…”

Section: Resultsmentioning

confidence: 98%

Brassinosteroids Antagonize Gibberellin- and Salicylate-Mediated Root Immunity in Rice

et al. 2012

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Brassinosteroids (BRs) are a unique class of plant steroid hormones that orchestrate myriad growth and developmental processes. Although BRs have long been known to protect plants from a suite of biotic and abiotic stresses, our understanding of the underlying molecular mechanisms is still rudimentary. Aiming to further decipher the molecular logic of BR-modulated immunity, we have examined the dynamics and impact of BRs during infection of rice (Oryza sativa) with the root oomycete Pythium graminicola. Challenging the prevailing view that BRs positively regulate plant innate immunity, we show that P. graminicola exploits BRs as virulence factors and hijacks the rice BR machinery to inflict disease. Moreover, we demonstrate that this immunesuppressive effect of BRs is due, at least in part, to negative cross talk with salicylic acid (SA) and gibberellic acid (GA) pathways. BR-mediated suppression of SA defenses occurred downstream of SA biosynthesis, but upstream of the master defense regulators NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 and OsWRKY45. In contrast, BR alleviated GA-directed immune responses by interfering at multiple levels with GA metabolism, resulting in indirect stabilization of the DELLA protein and central GA repressor SLENDER RICE1 (SLR1). Collectively, these data favor a model whereby P. graminicola coopts the plant BR pathway as a decoy to antagonize effectual SA-and GA-mediated defenses. Our results highlight the importance of BRs in modulating plant immunity and uncover pathogen-mediated manipulation of plant steroid homeostasis as a core virulence strategy.

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“…This may be related to inhibition of new gibberellin biosynthesis in the shoot. In arabidopsis, application of uniconazole, which is also a gibberellins inhibitor, caused shoot stunting, but with enhanced elongation of the primary roots (Bidadi et al 2009). In semidwarf rice (sd1) obtained from a mutation of the GA20ox-2 gene, the level of endogenous gibberellin was low, however, the normal height of the semidwarf rice is restored by exogenous application of GA (Ashikari et al 2002;Sasaki et al 2002).…”

Section: α-Amylase Activitymentioning

confidence: 99%

EFFECTS OF BLUE LIGHT AND PACLOBUTRAZOL ON SEED GERMINATION, VEGETATIVE GROWTH AND YIELD OF BLACK RICE (Oryza sativa L. 'Cempo Ireng')

Dewi

Agustina²,

Nurmalika³

2017

BIOTROPIA

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Black rice (Oryza sativa L. "Cempo Ireng") is one of local rice varieties in Sleman Regency, Yogyakarta. The black color is caused by high anthocyanin content which is important source of antioxidant. The cultivation of black rice is still limited due to its tall phenotype, long vegetative stage and low productivity compared to white rice. Paclobutrazol is a growth retardant causing dwarfing in several crop plants and reducing lodging. Blue light can improve plant quality. This research was aimed to evaluate the effect of blue light and paclobutrazol on seed germination, vegetative growth and yield of black rice. The results showed that the average of seed germination as well as the activity of α-amylase of seeds subjected to blue light were lower compared to those subjected to sunlight; however, paclobutrazol concentrations did not affect seed germination percentage. The height of rice plants treated with paclobutrazol decreased in accordance with the increase of paclobutrazol concentration. Chlorophyll content and tiller numbers increased by paclobutrazol treatment of 12.5 ppm. Nitrate reductase activity was higher in rice seedlings subjected to blue light compared to those subjected to sunlight. Iron (Fe) content of rice plants treated with 25 or 50 ppm paclobutrazol increased compared to control. It was concluded that paclobutrazol application of 12.5 ppm already reduced plant height. The higher concentration of paclobutrazol applied the greater reduction on plant height was observed. Blue light treatment applied during black rice seed germination slightly reduced germination percentage and α-amylase activity in the germinated seeds. However, blue light treatment combined with paclobutrazol application during black rice seed germination increased chlorophyll content, tiller numbers and Fe content in black rice grain.

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Effects of shoot-applied gibberellin/gibberellin-biosynthesis inhibitors on root growth and expression of gibberellin biosynthesis genes in Arabidopsis thaliana

Cited by 36 publications

References 32 publications

Effects of Gibberellin and Gibberellin Biosynthesis Inhibitor (Paclobutrazol) Applications on Radish (Raphanus sativus) Taproot Expansion and the Presence of Authentic Hormones

Effects of Gibberellin and Gibberellin Biosynthesis Inhibitor (Paclobutrazol) Applications on Radish (Raphanus sativus) Taproot Expansion and the Presence of Authentic Hormones

Brassinosteroids Antagonize Gibberellin- and Salicylate-Mediated Root Immunity in Rice

EFFECTS OF BLUE LIGHT AND PACLOBUTRAZOL ON SEED GERMINATION, VEGETATIVE GROWTH AND YIELD OF BLACK RICE (Oryza sativa L. 'Cempo Ireng')

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