Effects of Shock Waves on Tenocyte Proliferation and Extracellular Matrix Metabolism

Chao, Yuan-Hung; Tsuang, Yang Hwei; Sun, Jui Sheng; Chen, Li-Ting; Chiang, Yueh Feng; Wang, Chien-Chun; Chen, Ming Hong

doi:10.1016/j.ultrasmedbio.2007.11.002

Cited by 75 publications

(68 citation statements)

References 37 publications

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“…4 Chao et al reported collagen increase and accelerated cell growth after shock wave treatment in normal tenocytes, especially noting the increase in TGF-β1 and proliferating cell nuclear antigen. 3 In the current study, a significant decrease in certain ILs and MMPs were noted after shock wave treatment, possibly shedding light on another biological effect of ESWT. MMPs and ILs are markers of tendinopathy and may have a role in the pathomechanics of tendinopathy.…”

Section: Discussionsupporting

confidence: 51%

Effect of Extracorporeal Shock Wave Therapy on Cultured Tenocytes

Han¹,

Lee²,

Guyton³

et al. 2009

Foot Ankle Int

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Background: Little has been reported about the biologic effect of shock waves on human normal or pathologic tendon tissue. We hypothesized that inflammatory cytokine and MMP production would be down-regulated by shock wave stimulation. . IL-6 levels were higher in the diseased tenocytes as compared with normal tenocytes (44.10 ± 16.72 versus 0.21 ± 0.55 ng/ml, (p < 0.05). IL-1 levels in normal cells increased (2.24 ± 5.02 ng/ml to 9.31 ± 6.85 ng/ml) after shock wave treatment (p = 0.04). In diseased tenocytes, levels of MMP-1 (1.12 ± 0.23 to 0.75 ± 0.24 ng/ml; p = 0.04) and MMP-13 (1.43 ± 0.11 to 0.80 ± 0.15 ng/ml; p = 0.04) were significantly decreased after shock wave treatment. The IL-6 level in diseased tenocytes was decreased (44.10 ± 16.72 to 14.66 ±

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Section: Discussionsupporting

confidence: 51%

Effect of Extracorporeal Shock Wave Therapy on Cultured Tenocytes

Han¹,

Lee²,

Guyton³

et al. 2009

Foot Ankle Int

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“…More recently, studies showed that ESWT could enhance proliferation and collagen synthesis in cultured tenocytes and dermal fibroblasts (Chao et al 2008;Berta et al 2009). Considering that synthesis of collagen is essential to regain wound strength, we hypothesized that lowenergy ESWT would increase collagen content in wound tissue and thus accelerate the healing of incision in surgical patients with diabetes.…”

mentioning

confidence: 99%

Extracorporeal Shock Wave Treatment Improves Incisional Wound Healing in Diabetic Rats

Yang

Luo

Yan

et al. 2011

Tohoku J. Exp. Med.

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Impaired wound healing in surgical patients with diabetes increases the incidence of infection, prolongs hospitalization, and even increases the rate of mortality. Low-energy extracorporeal shock wave treatment (ESWT) was reported to accelerate chronic wound healing by promoting revascularization and tissue regeneration; however, it is not known if ESWT could also improve healing of acute surgical incisional wounds in diabetes. In this study, using a rat model of diabetes, we investigated the effect of low-energy ESWT on collagen content in wound tissues and its efficacy in incisional wound healing. A single dorsal incisional wound was inflicted in streptozotocin-induced diabetic rats, and they received ESWT at different time post-wounding. Rats were sacrificed on days 7 and 14 post-wounding. Wound breaking strength, hydroxyproline content, histological characteristics and the expression of transforming growth factor beta 1 (TGF-β1) were analyzed. As a result, the wound breaking strength was significantly enhanced and the hydroxyproline content in wound tissues was increased at each time point examined. The number of fibroblasts was significantly increased, and the new collagen fibers were more abundant at the wound site after ESWT. Furthermore, the expression of TGF-β1 was up-regulated after ESWT on day 7 post-wounding. These results suggest that low-energy ESWT can increase collagen content, enhance wound breaking strength and improve the healing of incisional wound in diabetic rats. The increased collagen content may be attributed, at least in part, to the up-regulation of TGF-β1 expression in wound tissues.

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“…In various experiments, proliferation-promoting effects of shockwave application were demonstrated previously in various cell types [6,7]. In addition to a predominantly cytotoxic effect of ESWT at high energy flux densities, increased proliferation at lower energy densities was shown for cultures of fibroblasts, tenocytes, periosteal cells and osteoblasts from human cancellous bone [9,[21][22][23]. With regard to keratinocytes specifically, mechanical stress sensitivity was demonstrated with a growth-promoting effect in strain tests, as well as promotion of differentiation in compression tests [24][25][26].…”

Section: Discussionmentioning

confidence: 73%

Extracorporeal Shockwaves (ESW) Promote Proliferation and Differentiation of Keratinocytes In vitro-Histology and Immunohistochemistry

Antonic

Hartmann²,

Münch³

et al. 2015

J Bioengineer & Biomedical Sci

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The objective of presented study was to evaluate in vitro effects of extracorporeal shockwave therapy (ESWT) on the keratinocyte morphology, cytoskeleton and mitotic activity. To determine in vitro effects of ESWT on keratinocytes, we applied 100 pulses with an energy flux density 0.1 mJ/mm 2 and a frequency of 1 Hz at a distance of 5 cm between therapy head and culture flask. The treatment parameters were determined in a pilot study. Haematoxylin and Eosin staining, as well as immunohistochemistry for Ki-67, CK5, CK14 and CK10 was performed on the cultured keratinocytes and results were compared between ESW treated group and Controls. No pathomorphological abnormalities or alterations in the cells monolayer could be observed. When assessing histological images of all HE-stained sections of ESW-treated and untreated keratinocytes (control group), no morphological differences could be seen between or within the groups. The proliferation marker Ki-67 was found at a frequency of 14% for ESW-treated and 10% for untreated keratinocytes. There were also figures of 95% (CK5 staining) and 90% (CK14 staining) for ESW-treated, as well as 85% (CK5 staining) and 85% (CK14 staining) for untreated keratinocytes. CK10-positive cells showed a frequency of 24% in the ESWtreated and 33% in the untreated cell cultures. Immunohistochemical observations indicated an increased proliferation behaviour following treatment with extracorporeal shockwaves in vitro and maturation of the exposed keratinocytes. Our results suggest an increase in proliferation activity of keratinocytes after in vitro treatment with ESWT. Further investigations of the effects of ESWT on the keratinocyte expression of the chemokines and cytokines are underway.

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Effects of Shock Waves on Tenocyte Proliferation and Extracellular Matrix Metabolism

Cited by 75 publications

References 37 publications

Effect of Extracorporeal Shock Wave Therapy on Cultured Tenocytes

Effect of Extracorporeal Shock Wave Therapy on Cultured Tenocytes

Extracorporeal Shock Wave Treatment Improves Incisional Wound Healing in Diabetic Rats

Extracorporeal Shockwaves (ESW) Promote Proliferation and Differentiation of Keratinocytes In vitro-Histology and Immunohistochemistry

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