Effects of sevoflurane on leucine-rich repeat kinase�2-associated Drosophila model of Parkinson's disease

Abstract

Abstract.Patients with Parkinson's disease (PD) often require surgery, and therefore may receive inhalation anesthesia. However, it is currently unknown whether inhalation anesthetics affect the prognosis of the disease. Leucine-rich repeat kinase 2 (LRRK2) genetic mutations are the most common cause of familial PD, contributing to ~39% of all cases in certain populations. The aim of the present study was to determine the effects of inhaled anesthetics on PD, by observing the influence of sevoflurane on a LRRK2-associated Drosophila model of PD. PD transgenic Drosophila overexpressing LRRK2 were generated by crossing flies expressing an LRRK2 upstream activation sequence, with tyrosine hydroxylase (TH)-Gal4 flies. Western blot analysis successfully verified that the transgenic Drosophila overexpressed LRRK2. Three days prior to eclosion, three genotypes of Drosophila were divided into four groups, and were exposed to air, 1, 2, or 3% sevoflurane, for 5 hours. Twenty-four hours after the exposure, the electrophysiological activities of the projection neurons (PN) in the brains of the Drosophila were recorded using a patch clamp. The locomotor activities were tested on days 5,10,15,20, 25,30,35 and 40 following eclosion. The frequency of miniature excitatory synaptic currents (mEPSCs) obtained from the PNs of the TH-wild type LRRK2 (TH-WT) Drosophila brain, following exposure to air (1.60±0.05 Hz), was lower as compared with the wild type LRRK2 (WT) (2.51±0.07 Hz) and W1118 (2.41±0.10 Hz) Drosophila. After exposure to 1, 2 and 3% sevoflurane, the frequency of mEPSCs in the brains of the TH-WT group decreased to 0.82±0.04 Hz, 0.63±0.16 Hz and 0.55±0.04 Hz, respectively. The percentage decrease of the frequency of mEPSCs, from exposure to air to 1% sevoflurane, of the TH-WT group (48.32%±3.08%) was significantly higher, as compared with the WT (39.17%±1.42%) and W1118 (35.10%±2.66%) groups, and there was no statistical difference between the WT and W1118 groups. The transgenic TH-WT Drosophila presented an early decrease in locomotor ability, as compared with the WT and W1118 groups. Following a 5 hour exposure to sevoflurane, the percentage decrease of the climbing abilities of the TH-WT group, from exposure to air to 1% sevoflurane, were significantly lower, as compared with the WT and W1118 groups. In conclusion, sevoflurane had negative effects on the control W1118 flies, and also severely aggravated the prognosis of PD in the LRRK2-associated Drosophila model, through synaptic cholinergic deficits and impairment on locomotor abilities.

Effects of anesthetic method on inflammatory response in patients with Parkinson’s disease: a randomized controlled study

“…We chose to use sevoflurane, one of the commonly used inhalational anesthetics, because it helps rapid induction and recovery. Shan et al [25] reported that sevoflurane aggravated the prognosis of PD in a Drosophila model. We attributed the lack of statistical significance in this study to the relatively short duration of DBS surgery and the follow-up due to short hospitalization period.…”

Effects of anesthetic method on inflammatory response in patients with Parkinson’s disease: a randomized controlled study

“…Previous studies have indicated that TIVA has superior effects in inhibiting inflammatory responses to inhalational anesthetics [9,[17][18][19]. Shan et al [20] reported that the inhalational anesthetic sevoflurane has a negative effect and aggravates the prognosis of PD in a Drosophila model. We attributed the lack of statistical significance in this study to the relatively short duration of surgery and the follow-up of only 24 h after surgery may not have been sufficient to reveal cytokine changes.…”

“…Shan et al [20] reported that impairment of locomotor abilities is aggravated with exposure to sevoflurane in a Drosophila PD model. Tuon et al [12] reported that IL-17 levels decrease after physical training in an experimental mouse model of PD.…”

Elevated expression of DJ-1 (encoded by the human PARK7 gene) protects neuronal cells from sevoflurane-induced neurotoxicity

“…Mason and colleagues showed that administration of sevoflurane changed the concentration of dopamine (DA) in the brain by impairing the transport synaptosomes of DA, thus leading to further damage of the dopaminergic system and locomotor dysfunction (Mason et al 1996). Additionally, sevoflurane severely aggravated the prognosis of PD in another causative gene LRRK2-associated Drosophila model by means of synaptic cholinergic deficits and impairment of locomotor abilities (Shan et al 2015). Interestingly, a recent study demonstrated that DJ-1 has the potential to protect neuronal cells against oxidative stress, mitochondrial dysfunction, and apoptosis induced by the inhaled anesthetic gas isoflurane (Liu et al 2015).…”