Effects of sevoflurane and propofol on hemodynamics, cerebral oxygen metabolism and analgesia during maintenance of paediatric anaesthesia

Purpose: To investigate the effect of eriocitrin on sevoflurane-induced neurotoxicity in mice.Methods: Mouse hippocampal neurons (HT22) were exposed to different concentrations of sevoflurane for 6 h and then incubated with different concentrations of eriocitrin for another 24 h. Cell viability was determined by CCK8 assay, while fluorescence intensity of dichlorodihydrofluorescein was used to evaluate reactive oxygen species. Enzyme linked immunosorbent assay (ELISA) was used to determine oxidative stress, and cellular apoptosis was determined by flow cytometry.Results: Sevoflurane exposure decreased HT22 cell viability, whereas incubation with eriocitrin increased viability of sevoflurane-treated HT22 cells (p < 0.05). Sevoflurane-induced increase in dichlorodihydrofluorescein fluorescence intensity was reduced by eriocitrin, but eriocitrin attenuated sevoflurane-induced increase in malondialdehyde, superoxide dismutase, and glutathione peroxidase in HT22 cells. Cell apoptosis increased after sevoflurane exposure, and eriocitrin suppressed apoptosis in sevoflurane-treated HT22 cells through downregulation of cleaved caspase-3 and cleaved caspase-9 (p< 0.05). Eriocitrin incubation enhanced protein expression of nuclear factor E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and NAD(P)H quinone dehydrogenase 1 (NQO1) in sevoflurane-treated HT22 cells (p < 0.05).Conclusion: Eriocitrin ameliorates sevoflurane-induced oxidative stress and inflammatory response in HT22 cells via activation of Nrf2/HO-1/NQO1 signaling. Thus, agent may be useful in the treatment of sevoflurane-induced toxicity, but in vivo studies are required to buttress this.

Section: Introductionmentioning

confidence: 99%

Eriocitrin alleviates sevoflurane-induced cytotoxicity in HT22 cells via Nrf2 pathway

Yuan¹,

Zhu²,

Wei³

et al. 2022

“…Interestingly, use of volatile anesthetics has been shown to prevent tissue damage after liver ischemia by regulating blood flow [7]. Sevoflurane is an inhaled anesthetic that functions as a bronchodilator by regulating calcium homeostasis [8,9]. Sevoflurane protects against lung [10] and myocardial [11] ischemia reperfusion injury.…”

Section: Introductionmentioning

confidence: 99%

Sevoflurane alleviates liver ischemia reperfusion injury through inactivation of the TRAF6/NF-κB signaling pathway

Liu¹,

Yuan²,

Rao³

2021

Purpose: To evaluate the role and mechanism of action of sevoflurane in liver ischemia reperfusion injury.Methods: Rats were pretreated with sevoflurane and then underwent liver ischemia followed by reperfusion to establish an animal model of liver ischemia reperfusion injury. Pathological changes in liver tissues were investigated by hematoxylin and eosin (H & E) staining, and serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined using a chemistryanalyzer. ELISA was used to determine the levels of myeloperoxidase (MPO), tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), IL-6, superoxide (SOD), malonaldehyde (MDA), catalase (CAT), and glutathione (GSH).Results: Pathological changes in liver tissue, including sinusoidal congestion, vacuole formation, and infiltration of inflammatory cells and lymphocytes, were identified in rats post-ischemia reperfusion injury. In addition, serum ALT and AST levels increased following ischemia reperfusion injury. However, administration of sevoflurane ameliorated the pathological liver damage and decreased the serum ALTand AST levels induced by ischemia reperfusion. Pro- inflammatory cytokines, such as MPO, TNF-α, IL- 1β, and IL-6 were upregulated in rats following ischemia reperfusion injury, and this upregulation was reversed by sevoflurane administration. Sevoflurane administration also attenuated the ischemia reperfusion-induced increase in MDA and decrease in SOD, CAT, and GSH. Ischemia reperfusionrepressed IκBα protein expression and promoted protein expression of TNF receptor associated factor 6 (TRAF6), phospho (p)-IκBα, and p-p65 in liver tissue. However, sevoflurane reversed the effect of ischemia reperfusion on IκBα, TRAF6, p-IκBα, and p-65 expression.Conclusion: Sevoflurane administration reduced pathological liver injury post-ischemia reperfusion bysuppressing the inflammatory response and oxidative stress through inactivation of the TRAF6/NF-κB pathway.

“…Pain management is an important anaesthetic care which is always debatable and challenging since it varies amongst individuals due to several factors [4]. Sevoflurane (Sev) or ultane is a poorly-soluble inhaled anaesthesia that is highly recommended as general anaesthesia for children or kids (inpatients and out-patients) because apart from its pleasant smell, it is associated with good early recovery and adequate hemodynamic stabilizing properties [5,6].…”

Section: Introductionmentioning

confidence: 99%

A comparative study of the analgesic effects of sevoflurane and propofol in children following otolaryngology surgical procedures: A pilot study

Yan-wu¹,

Fan²,

Ye³

et al. 2021

Purpose: To determine the analgesic effects of sevoflurane (Sev) and propofol (Pro) in children whounderwent otolaryngology surgical procedures, and their post-operative conditions.Methods: A total of 62 (ASA I or ASA II) pre-medicated children who were about to undergootolaryngology surgical procedures were chosen and divided equally into Sev and Pro groups, with 31patients per group. During the surgical procedure, Sev was administered via a mask, while Pro wasgiven i.v. Each anesthesia was followed with fentanyl administration.Results: Pain scores such as verbal rating scale (VRS) and visual analogue scale (VAS) were slightlylower in Sev group than in Pro group. However, post-operative conditions such as emergence delirium(ED) and emergence agitation (EA) were significantly elevated in Sev group, when compared to Progroup (p < 0.05). In addition, patients in Sev group had higher levels of hemodynamic parameters(blood pressure), and much higher number of adverse events than those in Pro group. Thus, the overallsatisfaction score and recovery characteristics, i.e., hospitalization time and recovery were slightlybetter in Pro-anesthetized children than in those given Sev.Conclusion: These results suggest that except for pain score, Pro-anesthetized children fared better interms of speedy recovery and reduced adverse effects than those given Pro. Thus, Pro may berecommended as general anaesthetic for children undergoing otolaryngology surgical procedures.Keywords: Sevoflurane, Propofol, Pain score, Emergence agitation, Otolaryngology