Effects of several snake venoms on serum and tissue transaminases, alkaline phosphatase and lactate dehydrogenase

Mohamed, A.H.; Fouad, Shaimaa H; Elaasar, Safaa; Salem, Ahmed M.; Abdelaal, Amr; Hassan, Amr; Zahran, Faten; Abbas, Naglaa F.

doi:10.1016/0041-0101(81)90098-2

Cited by 11 publications

(5 citation statements)

References 10 publications

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“…Shaban and Hafez (2003) reported that the LDH is one of the enzyme markers of such injured tissues by a snake envenomation also, the authors concluded that the i.p. injected snake venom caused a marked decrease of serum LDH activity, attributed the reduction of LDH activity to renal damage as well as to the inhibition of their activities caused by the venom as had been suggested by Mohamed et al (1981).…”

Section: Discussionmentioning

confidence: 62%

Histological, molecular and biochemical detection of renal injury after Echis pyramidum snake envenomation in rats

Al-Johany

Al-Sadoon

Moneim

et al. 2015

Saudi Journal of Biological Sciences

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Nephrotoxicity is a common sign of snake envenomation. The present work aimed to clarify the effect of intraperitoneal injection of 1/8 LD50 and 1/4 LD50 doses of Echis pyramidum snake venom on the renal tissue of rats after 2, 4 and 6 h from envenomation. Histopathological examination showed intense dose and time dependent abnormalities, including swelling glomerulus and tubular necrosis and damage as well as signs of intertubular medullary hemorrhage at early stages of envenomation. However, at late stages of envenomation by any of the doses under investigation, no intact renal corpuscles were recorded and complete lysis in renal corpuscles with ruptured Bowman's capsules was observed. Immunohistochemistry by immunohistochemical staining was used to test the protein expression of Bax in renal tissue of rats. The result showed that the expression of Bax in renal tissue sections of envenomated rats was increased according to dose and time-dependant manner. The isolation of DNA from the renal cells of envenomed rats pointed out to the occurrence of DNA fragmentation, which is another indicator for renal tissue injury especially after 6 h of 1/4 LD50 of E. pyramidum envenomation. Oxidative stress biomarkers malondialdehyde and nitrite/nitrate levels, antioxidant parameters; glutathione, total antioxidant capacity and catalase were assayed in renal tissue homogenates. The venom induced significant increase in the levels of malondialdehyde and nitrite/nitrate while the levels of glutathione, total antioxidant capacity and catalase were significantly decreased, especially after 6 h of envenomation. The results revealed that the E. pyramidum induced dose and time-dependant significant disturbances in the physiological parameters in the kidney. We conclude that the use of the immunohistochemical techniques, the detection of DNA integrity and oxidative stress marker estimations are more specific tools that can clarify cellular injury and could point out to the defense activity of the renal tissue at envenomation.

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Section: Discussionmentioning

confidence: 62%

Histological, molecular and biochemical detection of renal injury after Echis pyramidum snake envenomation in rats

Al-Johany

Al-Sadoon

Moneim

et al. 2015

Saudi Journal of Biological Sciences

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“…Ophidic venoms, including the Bothrops genus, can cause serious liver damage, usually resulting in the release of enzymes such as ALT, ALP and γ‐GT from the intracellular environmental to the blood plasma. Hepatotoxicity caused by acidic PLA 2 was first observed by Nisenbom et al, toxin from Bothrops alternatus venom capable of severely compromising the liver.…”

Section: Discussionmentioning

confidence: 99%

“…15,16 In this context, hepatic function can be analysed by determining the plasma levels of enzymes present in liver cells that are released into the blood in situations of liver injury. 49,50 Ophidic venoms, including the Bothrops genus, can cause serious liver damage, usually resulting in the release of enzymes such as ALT, ALP and γ-GT 15,51,52 from the intracellular environmental to the blood plasma. Hepatotoxicity caused by acidic PLA 2 was first observed by Nisenbom et al, 53 toxin from Bothrops alternatus venom capable of severely compromising the liver.…”

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confidence: 99%

Systemic alterations induced by phospholipase A₂, BmooTX‐I, isolated from Bothrops moojeni snake venom

Costa

Sousa

Dias

et al. 2018

Int J Experimental Path

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Summary Ophidic accidents are among the problems of public health in Brazil. The components from bothropic venom are responsible for many systemic clinical complications resulting from envenomation. The present work aimed to analyse the systemic changes induced in mice after intraperitoneal administration of BmooTX‐I, a myotoxic acidic phospholipase A2 isolated from Bothrops moojeni venom. Urinalysis was performed and the following plasma biochemical markers were documented: urea, creatinine and uric acid (renal function); glucose and amylase (pancreatic function); alanine aminotransferase, alkaline phosphatase and gamma‐GT (intra‐ and extrahepatic function); creatine kinase and enzymatic lactate (muscle function). Our results showed that after the intraperitoneal injection of BmooTX‐I the urine of these animals showed glycosuria, proteinuria, haematuria, bacteriuria, bilirubinuria, polyuria and nitrite. The plasma biochemical analysis showed alterations in levels of urea, creatinine and uric acid. Amylase concentration was not altered significantly, but the plasma glucose increased significantly compared to controls. The plasma levels of alanine aminotransferase and alkaline phosphatase decreased and increased, respectively, in these same animals. On the other hand, the plasma γGT concentration did not undergo significant modification compared to the control group. The plasma concentration of CK increased, while the enzymatic lactate concentration decreased after the injection of the BmooTX‐I. Therefore, in mice BmooTX‐I is capable of causing systemic alterations which manifest as renal, muscular, hepatic and pancreatic impairment.

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“…A ação tóxica sobre os hepatócitos é relevante manifestando-se por alterações das enzimas hepáticas como alanina, alaninatransferase, e a aminotransferase (Mohamed et al, 1981).…”

Section: Atividade Hepatotóxicaunclassified

Estudo da toxicidade das peçonhas crotálicas e botrópicas, no acidente ofídico, com ênfase a toxicidade renal

Castro

2006

Mundo Saúde

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Estudo da toxicidade das peçonhas crotálicas e botrópicas,no acidente ofídico, com ênfase a toxicidade renal Study of toxicity of crotalic and bothropic species in ofidic accidents, with an emphasis in renal toxicity Estudio de la toxicidad de las especies crotalicas y botrópicas en accidentes ofidicos, con énfasis en toxicidad renal RESUMO: Os acidentes ofídicos possuem uma taxa de letalidade mundial de 2,3%, no Brasil chega a 6%. Nos acidentes crotálicos e botrópicos podem ocorrer insuficiência renal aguda (IRA) causada principalmente por necrose tubular aguda (NTA). As princiapias frações tóxicas no veneno crotálico é a crotoxina e a fosfolipase A2, no veneno botrópico são as metaloproteinases e a botropsina. O veneno crotálico pode levar a alterações glomerulares e nos túbulos proximais, entre as principais causas da IRA por NTA consta a a rabdomiólise, já o veneno botrópico pode levar a formaçãode trombos, levando o rim a isquemia pela diminuição da perfusão sanguínea. O comprometimento renal por acidentes ofídicos por apresentar comprometimento sistêmico, deixa ainda obscura sobre a patogênese a ação direta do veneno, bem como da terapia com soro antiofídico que da mesma forma ainda não está esclarecido em sua ação em diminuir uma potencial ação direta do veneno em tecido renal.

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Effects of several snake venoms on serum and tissue transaminases, alkaline phosphatase and lactate dehydrogenase

Cited by 11 publications

References 10 publications

Histological, molecular and biochemical detection of renal injury after Echis pyramidum snake envenomation in rats

Histological, molecular and biochemical detection of renal injury after Echis pyramidum snake envenomation in rats

Systemic alterations induced by phospholipase A₂, BmooTX‐I, isolated from Bothrops moojeni snake venom

Estudo da toxicidade das peçonhas crotálicas e botrópicas, no acidente ofídico, com ênfase a toxicidade renal

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Effects of several snake venoms on serum and tissue transaminases, alkaline phosphatase and lactate dehydrogenase

Cited by 11 publications

References 10 publications

Histological, molecular and biochemical detection of renal injury after Echis pyramidum snake envenomation in rats

Histological, molecular and biochemical detection of renal injury after Echis pyramidum snake envenomation in rats

Systemic alterations induced by phospholipase A2, BmooTX‐I, isolated from Bothrops moojeni snake venom

Estudo da toxicidade das peçonhas crotálicas e botrópicas, no acidente ofídico, com ênfase a toxicidade renal

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Systemic alterations induced by phospholipase A₂, BmooTX‐I, isolated from Bothrops moojeni snake venom