Effects of sesamin on chondroitin sulfate proteoglycan synthesis induced by interleukin-1beta in human chondrocytes

Abstract

BackgroundNumerous studies have reported on the health benefits of sesamin, a major lignin found in sesame (S. indicum) seeds. Recently, sesamin was shown to have the ability to promote chondroitin sulfate proteoglycan synthesis in normal human chondrocytes. This study assesses the anti-inflammatory effect of sesamin on proteoglycans production in 3D chondrocyte cultures.MethodsTo evaluate the effects of sesamin on IL-1β-treated human articular chondrocytes (HAC) pellets, the pellets were pre-treated with IL-1β then cultured in the presence of various concentrations of sesamin for 21 days. During that period, the expression of IL-1β, glycosaminoglycans (GAGs) content and Chondroitin sulfate proteoglycans (CSPGs) synthesis genes (ACAN, XT-1, XT-2, CHSY1 and ChPF) was measured. The GAGs accumulation in the extracellular matrix was determined on day 21 by histological analysis.ResultsThere was clear evidence that sesamin upregulated expression of all the CSPGs synthesis genes, in contrast to the down-regulation of IL-1β expression both in genes and in protein levels. The level of release and matrix accumulation of GAGs in IL-1β pre-treated HAC pellets in the presence of sesamin was recovered. These results correlate with the histological examination which showed that sesamin enhanced matrix CSPGs accumulation.ConclusionsSesamin enhances CSPGs synthesis, suppresses IL-1β expression and ameliorates IL-1β induced inflammation in human chondrocytes. Sesamin could have therapeutic benefits for treating inflammation in osteoarthritis.

Sesamin Promotes Osteoporotic Fracture Healing by Activating Chondrogenesis and Angiogenesis Pathways

“…In our study, we proved that sesamin promoted chondrogenesis both in vitro and in vivo in the process of fracture healing. Sesamin was also found to enhance GAGs synthesis and alleviate IL-1β induced inflammation within human chondrocytes [ 35 ], which further supported our conclusion that sesamin supplement facilitated the endochondral ossification process during osteoporotic fracture healing.…”

Proinflammatory Effects of IL-1β Combined with IL-17A Promoted Cartilage Degradation and Suppressed Genes Associated with Cartilage Matrix Synthesis In Vitro

“…These genes regulate chondrogenesis and their expression levels are found to increase during cartilage development. A previous study showed that pretreatment of IL-1β in human articular chondrocyte pellets suppressed the expression of the ACAN gene and upregulated the expression of the catabolic genes [31]. Nevertheless, no report exists on the induction of the cytokine combination on the anabolic mechanisms, and the changes after cytokine withdrawal are still debatable.…”

Nutritional Composition of Grain and Seed Proteins

“…Sesame seeds are rich in B vitamins-niacin, thiamine, and vitamin B6, essential for proper cellular function and metabolism. Sesame seeds contain sesamin -a compound with antiinflammatory and antioxidant effects reported to soothe arthritic knee pain [97]. Ground sesame seed (sesame flour) can be used in smoothies, fish batter, baking, and more.…”