Effects of Serotonin Depletion by p-Chlorophenylalanine, p-Chloroamphetamine or 5,7-Dihydroxytryptamine on Central Dopaminergic Neurons: Focus on Tuberoinfundibular Dopaminergic Neurons and Serum Prolactin

Yang, Iris Chun-Hui; Pan, Jenn-Tser

doi:10.1159/000025386

Cited by 6 publications

(7 citation statements)

References 23 publications

(49 reference statements)

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“…However, the fact that a roughly 80% reduction in brain 5-HT did not even slightly attenuate the CRF-induced decrease in PPI in our studies suggests that CRF reduced PPI independently of its effects on 5-HT. PCPA treatment also decreased other monoamine levels, albeit to a lesser extent than 5-HT, as previously observed (Koe and Weissman, 1966;Yang and Pan, 1999). However, it does not appear that the effects of PCPA on DA, DOPAC, and NE altered the effects of CRF on PPI.…”

Section: Discussionsupporting

confidence: 77%

The effect of corticotropin-releasing factor on prepulse inhibition is independent of serotonin in Brown Norway and Wistar-Kyoto rats

Sutherland

Page

Conti

2008

Pharmacology Biochemistry and Behavior

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Prepulse inhibition (PPI), a form of sensorimotor gating, is reduced in a number of psychiatric disorders. Two experiments were conducted to determine whether corticotropin-releasing factor (CRF), which decreases PPI, does so via effects on serotonin (5-HT). Wistar Kyoto (WKY) and Brown Norway (BN) rats were used in both experiments in order to examine whether straindependent differences would be apparent in response to manipulations of the CRF and 5-HT systems. In the first experiment, WKY and BN rats received a subcutaneous injection of the 5-HT 2A/C receptor antagonist, ketanserin (2.0 mg/kg). Ten minutes later, rats received an intracerebroventricular (ICV) infusion of either 6.0 μl saline or CRF (0.3 μg or 3.0 μg). CRF decreased PPI despite blockade of 5-HT 2A/C receptors with ketanserin. In the second experiment, WKY and BN rats received an intraperitoneal injection of the 5-HT synthesis inhibitor, p-chlorophenylalanine (PCPA, 150 mg/kg), 48 and 24 hours prior to testing. On testing day, rats received an ICV infusion of either 6.0 μl saline or CRF (0.3 μg or 3.0 μg). CRF decreased PPI despite 5-HT depletion. These findings suggest that CRF does not decrease PPI via effects on 5-HT, since neither blockade of 5-HT 2A/C receptors nor 5-HT depletion attenuated this decrease.

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Section: Discussionsupporting

confidence: 77%

The effect of corticotropin-releasing factor on prepulse inhibition is independent of serotonin in Brown Norway and Wistar-Kyoto rats

Sutherland

Page

Conti

2008

Pharmacology Biochemistry and Behavior

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“…The systemic administration of PCPA into pregnant rats, and hence to fetuses, may raise certain difficulties in interpretation of results, e.g. because of side‐effects on the metabolism of catecholamines (Reader & Gauthier, 1984; Yang & Pan, 1999), which are potent inductors of brain development (Whitaker‐Azmitia et al ., 1996). Nevertheless, a more precise pharmacological method of intervention in fetal brain metabolism is not available at present.…”

Section: Discussionmentioning

confidence: 99%

Embryonic depletion of serotonin affects cortical development

Vitalis

Cases

Passemard

et al. 2007

Eur J of Neuroscience

137

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Compelling evidence suggests that serotonin (5-HT) is necessary for the refined organization of the cerebral cortex. Here we sought to analyse the short- and long-term consequences of embryonic 5-HT depletion on the development of the cerebral neocortex of the rat. We focused on the migration and differentiation of the pyramidal (projection) and nonpyramidal (interneuron) neuronal populations. Our paradigm used daily injection of DL-P-chlorophenylalanine (PCPA), a reversible inhibitor of 5-HT synthesis, during the E12-17 stage of embryonic development, when major events in corticogenesis take place. We monitored the 5-HT depletion induced by this treatment and showed that it led to subtle alterations in both the pyramidal and nonpyramidal neuronal populations. We found that E12-17 PCPA treatment altered the maturation of pyramidal neurons of layers III and V of the somatosensory cortex, with these cells displaying reduced dendritic arborization and complexity. These long-lasting alterations were not associated with modification of cortical BDNF levels at postnatal stages. We also showed that PCPA treatment transiently altered the incorporation in the cortical plate of interneurons derived from the caudal ganglionic eminence, and persistently affected the differentiation of a subpopulation expressing calretinin and/or cholecystokinin.

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“…5-HT can exhibit either stimulatory or inhibitory effects on nigrostriatal and mesolimbic DA neuronal activity [29, 30, 31]. Nevertheless, depletion of central 5-HT to a significant extent by 5,7-DHT had no significant effect on central DA neuronal activities, as shown in this and a previous study [32]. This finding does not necessarily speak against the notion that 5-HT has an effect on DA neurons.…”

Section: Discussionmentioning

confidence: 52%

An Endogenous Serotonergic Rhythm Acting on 5-HT<sub>2A</sub> Receptors May Be Involved in the Diurnal Changes in Tuberoinfundibular Dopaminergic Neuronal Activity and Prolactin Secretion in Female Rats

Liang¹,

Pan²

2000

Neuroendocrinology

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The central serotonergic system has long been known to have a stimulatory role on the secretion of prolactin (PRL). The integrity of serotonergic neurotransmission is essential for the expression of the estrogen-induced afternoon PRL surge. Whether its effect on PRL involves change in the activity of tuberoinfundibular dopaminergic (TIDA) neurons has not been ascertained. In adult ovariectomized rats treated with estrogen, depletion of central serotonin (5-HT) by 5,7-dihydroxytryptamine (5,7-DHT, 200 µg/rat, i.c.v.) effectively prevented the afternoon fall in TIDA neuronal activity (using the levels of 3,4-dihydroxyphenylalanine and 3,4-dihydroxyphenylacetic acid (DOPAC), and the ratio of DOPAC/dopamine in the median eminence as indices), and blunted the afternoon PRL surge. A single injection of a 5-HT_2A receptor antagonist, ketanserin (5 mg/kg, i.p. at 12.00 h), also had the same effects on the diurnal changes in TIDA neuronal activity and PRL secretion as the treatment with 5,7-DHT did. Intracerebroventricular injection of a 5-HT₂ receptor agonist 2,5-dimethoxy-4-iodoamphetamine (DOI) in the morning inhibited the TIDA neuronal activity and stimulated PRL secretion in a dose-dependent manner; while injection of a 5-HT₁ agonist, 8-hydroxy-dipropylaminotetralin, was without effect. Injection of DOI in 5,7-DHT-pretreated rats at 14.30 h also lowered the TIDA neuronal activity and reinstated the PRL surge. In all, endogenous 5-HT, acting through the 5-HT_2A receptor, appears to exhibit an inhibitory effect on TIDA neuronal activity during the afternoon, which is essential for the PRL surge.

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Effects of Serotonin Depletion by p-Chlorophenylalanine, p-Chloroamphetamine or 5,7-Dihydroxytryptamine on Central Dopaminergic Neurons: Focus on Tuberoinfundibular Dopaminergic Neurons and Serum Prolactin

Cited by 6 publications

References 23 publications

The effect of corticotropin-releasing factor on prepulse inhibition is independent of serotonin in Brown Norway and Wistar-Kyoto rats

The effect of corticotropin-releasing factor on prepulse inhibition is independent of serotonin in Brown Norway and Wistar-Kyoto rats

Embryonic depletion of serotonin affects cortical development

An Endogenous Serotonergic Rhythm Acting on 5-HT<sub>2A</sub> Receptors May Be Involved in the Diurnal Changes in Tuberoinfundibular Dopaminergic Neuronal Activity and Prolactin Secretion in Female Rats

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