Effects of sericin on oxidative stress and PI3K/AKT/mTOR signal pathway in cryopreserved mice ovarian tissue

Shu, Weihan; Yang, Song; Wei, Meng; Liu, Xiao Chao; Chen, Zi Xuan; Wei, Chen Yang; Zhang, Xin; Na, Li; Chen, Zhi Hong; Qiao, Yue; Li, Dong Mei; Sun, Tie; Cheng, Lu

doi:10.1016/j.cryobiol.2023.03.003

Cited by 2 publications

(1 citation statement)

References 55 publications

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“…Mice ovaries were cryopreserved according to our previous protocol (Shu et al, 2023). Cryopreserved solutions were (A)10 mg/mL BSA (Solarbio, A8010, Beijing, China), 0.25 M sucrose (Solarbio, A8271, Beijing, China), 10% DMSO (Solarbio, D8371, Beijing, China), 10% ethylene glycol (Solarbio, E8780, Beijing, China) in TCM199 medium (Gibco, 12340030, CA, USA), and (B) 10 mg/mL BSA, 0.25 M sucrose, 20% DMSO, 20% ethylene glycol in TCM199 medium.…”

Section: Ovarian Tissue Cryopreservation and Warmingmentioning

confidence: 99%

Melatonin activates Nrf2/HO‐1 signalling pathway to antagonizes oxidative stress‐induced injury via melatonin receptor 1 (MT1) in cryopreserved mice ovarian tissue

Wei,

Zhang,

et al. 2024

Reprod Domestic Animals

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Our previous research has shown that melatonin (MLT) can reduce cryopreserved ovarian damage in mice. Yet, the molecular mechanism of MLT protection is still unclear. Some studies have shown that melatonin receptor 1 (MT1) is very important for animal reproductive system. To evaluate whether MLT exerts its protective effect on cryopreserved mice ovarian tissue via MT1, we added antagonist of MT1/MT2 (Luzindor) or antagonist of MT2 (4P‐PDOT) to the freezing solution, followed by cryopreservation and thawing of ovarian tissue. The levels of total superoxide dismutase (T‐SOD), catalase (CAT), nitric oxide (NO) and malondialdehyde (MDA) were detected. Besides, by using RT‐PCR and Western blotting, the expression of Bcl‐2, Bax and Nrf2/HO‐1 signalling pathway‐related proteins was detected. These findings demonstrated that compared with the melatonin group, the addition of Luzindor increased apoptosis, NO and MDA activities, decreased CAT and T‐SOD activities and inhibited Nrf2/HO‐1 signalling pathway. In conclusion, melatonin can play a protective role in cryopreserved ovarian tissue of mice through MT1 receptor.

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Section: Ovarian Tissue Cryopreservation and Warmingmentioning

confidence: 99%

Melatonin activates Nrf2/HO‐1 signalling pathway to antagonizes oxidative stress‐induced injury via melatonin receptor 1 (MT1) in cryopreserved mice ovarian tissue

Wei,

Zhang,

et al. 2024

Reprod Domestic Animals

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Sericin alleviates motor dysfunction by modulating inflammation and TrkB/BDNF signaling pathway in the rotenone-induced Parkinson’s disease model

Salari,

Ashabi,

Fartoosi

et al. 2023

BMC Pharmacol Toxicol

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BackgroundParkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by the degeneration of nigrostriatal dopaminergic neurons and movement impairment. Based on theories, neuroinflammatory processes may be vital in the etiology of PD and other neurodegenerative diseases. Reports show that rotenone has neurotoxic, inflammatory, and motor impairment effects in PD. Sericin is a natural polymer with effective properties, such as neuroprotective and anti-inflammatory. Therefore, this study aimed to examine the effects of sericin administration on motor dysfunction by modulating inflammation and tyrosine kinase B/brain-derived neurotrophic factor (TrkB/BDNF) pathway in the rotenone-induced PD model.MethodsWistar male rats (3-months-old) were treated with rotenone (2 mg/kg every 48 h for 30 days) to induce a rotenone-induced PD model. Also, sericin was administered orally at dose of 200 mg/kg every 48 h for 30 days. Rotarod and bar tests were performed for motor dysfunction. The protein levels of BDNF, c-fos, TrkB, tumor necrosis factor- α (TNF-α), interleukin-6 (IL-6) and catalase activity were evaluated in the striatum area.ResultsResults showed that sericin increased latent time in the rotarod test and decreased the time staying on the pole in the bar test compared to the PD group (P < 0.001 for both tests). Moreover, sericin treatments decreased TNF-α (P < 0.001) and IL-6 (P < 0.001) concentration levels and enhanced the levels of BDNF (P < 0.001), c-fos (P < 0.001), TrkB (P < 0.001) proteins and catalase activity (P < 0.05) in the striatum area compared to the PD group.ConclusionThese results support a protective benefit of sericin therapy in a rotenone-induced PD paradigm by reducing motor impairment, inflammatory response, and disruption of the TrkB/BDNF signaling pathway.

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Effects of sericin on oxidative stress and PI3K/AKT/mTOR signal pathway in cryopreserved mice ovarian tissue

Cited by 2 publications

References 55 publications

Melatonin activates Nrf2/HO‐1 signalling pathway to antagonizes oxidative stress‐induced injury via melatonin receptor 1 (MT1) in cryopreserved mice ovarian tissue

Melatonin activates Nrf2/HO‐1 signalling pathway to antagonizes oxidative stress‐induced injury via melatonin receptor 1 (MT1) in cryopreserved mice ovarian tissue

Sericin alleviates motor dysfunction by modulating inflammation and TrkB/BDNF signaling pathway in the rotenone-induced Parkinson’s disease model

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