Effects of serial passage of Autographa Californica nuclear polyhedrosis virus in cell culture

Kumar, Suresh; Miller, Lois K.

doi:10.1016/0168-1702(87)90047-5

Cited by 86 publications

(54 citation statements)

References 21 publications

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“…Part B is the same as part A except that less DNA was loaded in each lane to improve the resolution of the higher molecular weight DNA fragments. 13.7, 12.9, 12.7,6.4,2.7, 2, and 1.2 kb were present in both digests. Fragments of 16.5, 11.5, and 2.7 kb and fragments of 28 and 3.5 kb were specific to isolates 5-6 and A2-1, respectively.…”

Section: Potency Determinationsmentioning

confidence: 89%

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Properties of two Lymantria dispar nuclear polyhedrosis virus isolates obtained from the microbial pesticide Gypchek

Slavicek

Podgwaite

Lanner-Herrera

1992

Journal of Invertebrate Pathology

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Section: Potency Determinationsmentioning

confidence: 89%

“…Typically, these insertions are in the same approximate genomic location, suggesting the presence of preferential insertion sites (7,9,13). The disruption of a single gene has been shown to be sufficient to generate the FP phenotype in AcMNPV (1).…”

Section: Introductionmentioning

confidence: 99%

Properties of two Lymantria dispar nuclear polyhedrosis virus isolates obtained from the microbial pesticide Gypchek

Slavicek

Podgwaite

Lanner-Herrera

1992

Journal of Invertebrate Pathology

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“…Although genotypes with deletions in the egt gene occur spontaneously in tissue culture stocks of AcMNPV (Kumar & Miller, 1987;O'Reilly et al, 1990), there is no direct evidence that egt mutations provide a growth advantage in tissue culture. It is possible that the deletion genotypes represented by the plaque isolates described in this study are variant genotypes that occur naturally in Missouri field isolates of SfMNPV.…”

Section: Sfmnpv Genome Sequencementioning

confidence: 99%

Genomic sequence analysis of a fast-killing isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus

Harrison

Puttler

Popham

2008

Journal of General Virology

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Six clones of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) were plaquepurified from field isolates collected in Missouri, USA. In bioassays, four of the plaque-purified isolates killed neonate S. frugiperda larvae more rapidly than the field isolates from which they were derived, with LT 50 values (mean time to kill 50 % of the test larvae) ranging from 34.4 to 49.7 h post-infection. The complete genomic sequence of one of these isolates, SfMNPV-3AP2, was determined and analysed. The SfMNPV-3AP2 genome was 131 330 bp with a G+C content of 40.2 %. A total of 144 open reading frames (ORFs) was identified and examined, including the set of 62 genes in common among lepidopteran nucleopolyhedrovirus genomes. Comparisons of ORF content, order and predicted amino acid sequences with other nucleopolyhedoviruses indicated that SfMNPV is part of a cluster of viruses within NPV group II that includes NPVs isolated from Spodoptera, Agrotis and Mamestra host species. SfMNPV-3AP2 shared a high degree of nucleotide sequence similarity with partial sequences from other SfMNPV isolates. Comparison of the SfMNPV-3AP2 genome sequence with a partial sequence from a Brazilian isolate of SfMNPV revealed that SfMNPV-3AP2 contained a deletion that removed parts of ORF sf27 and the gene encoding ecdysteroid UDP-glucosyltransferase (egt). An examination of the egt region in the other isolates revealed that the other five SfMNPV clones also contained deletions of varying length in this region. Variant genotypes with deletions extending around the egt gene have been reported previously from a Nicaraguan field isolate of SfMNPV, suggesting that the presence of such variants is a common feature of SfMNPV populations.

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“…The amino acid sequences of wild-type FP25K and both the D22P and L36P mutants were entered into the COILS server (http://www.ch.embnet.org/software/COILS_form.html), and the coiled-coil structure of these proteins was predicted (37). The output for this prediction is represented by graphical plots, with all residue-scanning windows (14,21,28) containing the probability of a coiledcoil structure based on similarity to structures in a database of known coiled-coil proteins. High-probability peaks in these plots indicate the likelihood of a coiled-coil structure in that region.…”

Section: Methodsmentioning

confidence: 99%

“…Since it is not essential in vitro, and inactivation provides a selective advantage, the fp25k gene of baculoviruses readily undergoes mutation by transposition or replication slippage errors resulting in a base insertion or deletion (indel) during infection in cell culture (14,(20)(21)(22). These mutations lead to an evident fewpolyhedra (FP) phenotype, characterized by decreased production of OBs, fewer ODV envelope proteins localized to the nucleus, and increased BV titers (18,(22)(23)(24)(25).…”

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confidence: 99%

Baculovirus FP25K Localization: Role of the Coiled-Coil Domain

Garretson

McCoy

Cheng

2016

J Virol

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Two types of viruses are produced during the baculovirus life cycle: budded virus (BV) and occlusion-derived virus (ODV). A particular baculovirus protein, FP25K, is involved in the switch from BV to ODV production. Previously, FP25K from the model alphabaculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) was shown to traffic ODV envelope proteins. However, FP25K localization and the domains involved are inconclusive. Here we used a quantitative approach to study FP25K subcellular localization during infection using an AcMNPV bacmid virus that produces a functional AcMNPV FP25K-green fluorescent protein (GFP) fusion protein. During cell infection, FP25K-GFP localized primarily to the cytoplasm, particularly amorphous structures, with a small fraction being localized in the nucleus. To investigate the sequences involved in FP25K localization, an alignment of baculovirus FP25K sequences revealed that the N-terminal putative coiled-coil domain is present in all alphabaculoviruses but absent in betabaculoviruses. Structural prediction indicated a strong relatedness of AcMNPV FP25K to long interspersed element 1 (LINE-1) open reading frame 1 protein (ORF1p), which contains an N-terminal coiled-coil domain responsible for cytoplasmic retention. Point mutations and deletions of this domain lead to a change in AcMNPV FP25K localization from cytoplasmic to nuclear. The coiled-coil and C-terminal deletion viruses increased BV production. Furthermore, a betabaculovirus FP25K protein lacking this N-terminal coiled-coil domain localized predominantly to the nucleus and exhibited increased BV production. These data suggest that the acquisition of this N-terminal coiled-coil domain in FP25K is important for the evolution of alphabaculoviruses. Moreover, with the divergence of preocclusion nuclear membrane breakdown in betabaculoviruses and membrane integrity in alphabaculoviruses, this domain represents an alphabaculovirus adaptation for nuclear trafficking of occlusion-associated proteins. IMPORTANCEBaculovirus infection produces two forms of viruses: BV and ODV. Manufacturing of ODV involves trafficking of envelope proteins to the inner nuclear membrane, mediated partly through the FP25K protein. Since FP25K is present in alpha-, beta-, and gammabaculoviruses, it is uncertain if this trafficking function is conserved. In this study, we looked at alpha-and betabaculovirus FP25K trafficking by its localization. Alphabaculovirus FP25K localized primarily to the cytoplasm, whereas betabaculovirus FP25K localized to the nucleus. We found that an N-terminal coiled-coil domain present in all alphabaculovirus FP25K proteins, but absent in betabaculovirus FP25K, was critical for alphabaculovirus FP25K cytoplasmic localization. We believe that this represents an evolutionary process that partly led to the gain of function of this N-terminal coiled-coil domain in alphabaculovirus FP25K to aid in nuclear trafficking of occlusion-associated proteins. Due to betabaculovirus breakdown of the nuclear membrane be...

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Effects of serial passage of Autographa Californica nuclear polyhedrosis virus in cell culture

Cited by 86 publications

References 21 publications

Properties of two Lymantria dispar nuclear polyhedrosis virus isolates obtained from the microbial pesticide Gypchek

Properties of two Lymantria dispar nuclear polyhedrosis virus isolates obtained from the microbial pesticide Gypchek

Genomic sequence analysis of a fast-killing isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus

Baculovirus FP25K Localization: Role of the Coiled-Coil Domain

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