Effects of seminal plasma and sperm motility factors on viability of epididymal sperm of stallions

Pasquini, D.F.; Melo, Cely Marini; Papa, Frederico Ozanam; Fioratti, Eduardo Gorzoni; Landim-Alvarenga, F. C.; Alvarenga, Marco Antônio; Zahn, Fabíola Soares; Vita, Bruna De; Dell’Aqua, J.A.

doi:10.1016/j.anireprosci.2008.05.115

Cited by 6 publications

(6 citation statements)

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“…These benefits were also observed in previous studies with cauda epididymal sperm (Pasquini et al, 2008) and with epididymal sperm from sub-fertile stallions (Melo et al, 2009). Previous studies from our laboratory also revealed an improvement of equine epididymal sperm after incubation with Sperm-Talp and Fert-Talp before freezing (Pasquini et al, 2008).…”

Section: Discussionsupporting

confidence: 71%

“…However the in vivo fertility of cauda epididymal spermatozoa tends to be low (Morris et al, 2002). Pasquini et al (2008) compared the influence of motility-enhancing media on the freezability of epididymal sperm in horses and concluded that incubation in Talp + progesterone, Fert-Talp or Sperm-Talp media, commonly used in IVF procedures, improved equine epididymal sperm motility after freezing. Melo et al (2009) evaluated the influence of those substances on epididymal sperm from sub-fertile stallions after freezing, and verified a pre-and post-thaw motility improvement over frozen ejaculated semen when samples were incubated with Sperm-Talp and with Fert-Talp.…”

Section: Introductionmentioning

confidence: 99%

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Influence of sperm motility factors on spermatozoa obtained from different epididymal segments

2010

Animal Reproduction Science

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Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Influence of sperm motility factors on spermatozoa obtained from different epididymal segments

2010

Animal Reproduction Science

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“…These authors also found no difference in sperm kinetics (MT, MP, VCL, and RAP) when SP was removed, and they reported semen filtration was as efficient as centrifugation at Â600g for 10 minutes in the maintenance of sperm viability. A study using equine epididymal semen previously incubated with BotuSêmen (BotuPharma, Botucatu, Brazil) or SP and centrifuged at Â600g for 10 minutes before cryopreservation reported no difference in MT, MP, or RAP between the two groups during post-thaw evaluation [35]. Table 2 Rate of development (mean and standard error of the mean) of in vitro-produced bovine embryos using semen of the control, centrifugation, and filtration groups.…”

Section: Discussionmentioning

confidence: 99%

“…Regarding mitochondrial membrane potential, no difference was observed between groups as expected, showing that the presence or absence of SP does not alter this parameter. Pasquini et al [35] also found no difference in this parameter when epididymal semen of stallions was incubated only with SP or BotuSêmen diluent for 15 minutes, followed by centrifugation and cryopreservation. Tanghe et al [50] reported that the percentage of spermatozoa with high mitochondrial activity evaluated by fluorescence microscopy was moderately correlated with IVF outcome (0.30 < r < 0.50).…”

Section: Groupmentioning

confidence: 93%

Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility

et al. 2017

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Cryopreservation of bull semen is a common biotechnology procedure in cattle breeding. However, when the ejaculate is obtained by electroejaculation, wide variation is observed in the sperm/seminal plasma (SP) ratio that can affect the freezability of semen in this species. The removal of SP may improve the quality of frozen bull semen. The objective of this study was to evaluate the effect of SP removal from the ejaculate on the cryopreservation of semen from 38 Nellore bulls collected by electroejaculation. After collection, the ejaculate was divided into three aliquots: (1) control (N) diluted to a concentration of 60 × 10 spermatozoa/mL and frozen with SP; (2) centrifugation (C) at ×600g for 10 minutes and the pellet resuspended and frozen at the same concentration as N; and (3) filtration (F) through SpermFilter and sperm recovered and frozen at the same concentration as N. After thawing, sperm kinetics, plasma and acrosome membrane integrity, mitochondrial membrane potential, oxidative stress, and in vitro fertility were evaluated. Statistical analysis was performed using the SAS 9.2 package, and differences were considered significant when P < 0.05. Higher average path velocity and straight-line velocity were observed in the groups submitted to SP removal compared to the control group (P < 0.01). In contrast, filtered samples exhibited higher beat cross frequency, straightness, and linearity compared to the other groups. Plasma membrane integrity was reduced when SP was removed, but lower oxidative stress was observed in groups C and F (34.91 ± 2.95% and 31.63 ± 2.95%, respectively) compared to group N (57.39 ± 2.95%). However, the percentage of hatched blastocysts was similar in the N and F groups (21.22 ± 1.05% and 24.00 ± 1.05%, respectively) and higher compared to group C (18.83 ± 1.05%). In conclusion, removal of SP by centrifugation for bull semen freezing reduced the rate of in vitro-produced embryos, whereas filtration of prefrozen semen was found to be an efficient alternative in terms of semen freezability and in vitro production of bovine embryos.

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“…Some authors have studied the effects of exposing epididymal spermatozoa to SP before freezing on post‐thaw sperm parameters 4,15,17‐19 . In most studies the frozen‐thawed spermatozoa exposed to SP showed similar values of seminal parameters (motility, viability, morphology, etc) to those of control groups 4 .…”

Section: Introductionmentioning

confidence: 99%

Seminal plasma components from fertile stallions involved in the epididymal sperm freezability

et al. 2020

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Background Seminal plasma (SP) plays a crucial role in sperm protection and functionality. However, the effect of SP on the sperm cryopreservation is dependent on the stallion and SP composition. The use of epididymal spermatozoa incubated in the presence of SP could help the identification of the components of SP that are able to confer protection upon the spermatozoa during freezing. Objective The aims of this study were (i) to identify SP components involved in the potential protection of epididymal spermatozoa during the freeze‐thawing process and (ii) to identify and evaluate the proteins likely related to sperm freezability, using two‐dimensional difference gel electrophoresis (2D‐DIGE). Materials and Methods Epididymal spermatozoa from 4 stallions were incubated with SP (80%, v/v) or without SP (control) before freezing. Sperm parameters were evaluated after thawing (viability, chromatin condensation, acrosomal integrity, reactive oxygen species [ROS]) and SP composition: total antioxidant capacity (TAC), fatty acid composition, total protein concentration, and protein components by 2D‐DIGE. Results After thawing, the proportions of viable and acrosome‐intact spermatozoa were higher than control when SP from two stallions was used (F and O). The SP of all stallions reduced ROS production in comparison with the control. After analyzing the SP components, it was found that total protein concentration, TAC, polyunsaturated fatty acids (PUFA), and eight specific proteins identified by 2D‐DIGE were different between stallions. Discussion These studies allow the identification of SP components that could be involved in sperm protection or cryotolerance. Use of this information could help in the selection of stallions according to their semen freezing capacity. Conclusion The composition of the SP probably contributes to semen cryotolerance capacity. Total protein, TAC, PUFA, and some proteins such as cysteine‐rich secreted protein 3 could be used as biomarkers for the selection for sperm cryotolerance.

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Effects of seminal plasma and sperm motility factors on viability of epididymal sperm of stallions

Cited by 6 publications

References 0 publications

Influence of sperm motility factors on spermatozoa obtained from different epididymal segments

Influence of sperm motility factors on spermatozoa obtained from different epididymal segments

Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility

Seminal plasma components from fertile stallions involved in the epididymal sperm freezability

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