Effects of Selenium with Vitamin E and Melatonin on Cadmium-Induced Oxidative Damage in Rat Liver and Kidneys

Abstract

The present study was performed to determine the protective effects of melatonin alone and vitamin E with selenium combination against cadmium-induced oxidative damage in rat liver. A total of 60 male rats were equally divided into five groups, one of which acted as control receiving subcutaneous injections of physiological saline. The remaining four groups were treated with subcutaneous injections of cadmium chloride at a dose of 1 mg/kg weight. The first study group received no treatment. The second group was treated with a combination of 60 mg/kg vitamin E and 1 mg/kg sodium selenite. Group 3 was treated with 10 mg/kg melatonin, and the four group received a combination of vitamin E, sodium selenite, and melatonin at the doses mentioned above. After 1 month, the animals were killed, and liver and kidneys were excised for histopathological inspection and determination of tissue malondialdehyde and the activity of superoxide dismutase. The animals receiving no treatment showed significantly higher malondialdehyde levels and reduced activity of superoxide dismutase (p < 0.05). Treatment with antioxidants resulted in a significant reduction in malondialdehyde when compared to nontreated animals (p < 0.05) and increase in the enzyme activity that was almost the same as the controls. The pathological findings were also in parallel with the results of the biochemical analysis. In conclusion, all the agents tested had protective effects against cadmium-induced oxidative damage.

Thymoquinone Ameliorates Cadmium-Induced Nephrotoxicity, Apoptosis, and Oxidative Stress in Rats is Based on its Anti-Apoptotic and Anti-Oxidant Properties

“…They also showed a decrease in lipid peroxidation that increased after Cd exposure [2,31,[53][54][55]. All these studies indicate that anti-oxidant agents reduced oxidative stress and cellular damage caused by Cd.…”

Psoroptes cuniculi induced oxidative imbalance in rabbits and its alleviation by using vitamins A, D3, E, and H as adjunctive remedial

“…β-carotene has significant antioxidant activity and can effectively quench the free radicals (Sharma and Sharma 2009). As a hydrophobic antioxidant that incorporates into lipid environments, vitamin E significantly decreases lipid peroxidation in different organs and body fluids (Kara et al 2008). Potential antioxidants include either natural free radical scavenging antioxidant enzymes or the agents which are capable of augmenting the activities of these enzymes, including the GSH-Px, GST, SOD, and CAT.…”

Cadmium stress: an oxidative challenge

“…a-tocopherol is the most abundant and active isoform in human and animal tissues (Packer et al 2001). As a hydrophobic antioxidant that incorporates into lipid environments, vit E significantly decreases Cd-induced lipid peroxidation in different organs and body fluids of rats (El-Demerdash et al 2004;Kara et al 2008;Karabulut-Bulan et al 2008). Exact mechanisms of uptake, distribution and cellular effects still have to be elucidated.…”