Effects of selenium supplementation on expression of SEPP1 in mRNA and protein levels in subjects with and without metabolic syndrome suffering from coronary artery disease: Selenegene study a double‐blind randomized controlled trial

Abstract

Selenoprotein P (SePP) is involved in the protection against diseases. The present study is the first investigation of the effect of selenium supplementation on plasma selenium and expression of SEPP1 in mRNA and protein levels based on metabolic syndrome (MetS), in individuals suffering from coronary artery diseases. In this clinical trial, 160 patients with angiographically documented stenosis of more than 75% in each vessel were enrolled. Patients received either 200-mg selenium yeast tablets or placebo tablets orally after a meal, once daily for 60 days. The mRNA and protein levels of the selenium and SePP1 products were determined before and after the study. From the initial 160 participants, 145 subjects (71 MetS-affected individuals, 74 MetS-unaffected individuals) enrolled in this study. Comparing the selenium and placebo groups, no significant percentage changes of plasma selenium, △Ct SEPP1, or SePP were shown (P > 0.05). Moreover, beyond a significant difference for the expression of SePP in the selenium group compared to its baseline level (P < 0.05), no other significant differences were revealed for plasma selenium and △Ct SEPP1 after the intervention in either group (P > 0.05). Selenium supplementation did not affect plasma selenium or the mRNA or protein level of SePP in either groups after a 2-months intervention beyond a significant increase of SePP in the MetS group. This trial suggests that further studies should investigate the long-term use of selenium supplementation and the effect of a SePP increase on MetS as a potential therapeutic effect.

<i>Coix lacryma-jobi</i> Seed Oil Reduces Fat Accumulation in Nonalcoholic Fatty Liver Disease by Inhibiting the Activation of the p-AMPK/SePP1/apoER2 Pathway

“…A study of Sep-deficient mice found that mice had elevated glucose tolerance and increased insulin sensitivity in liver and muscle tissue compared to normal mice, but intraperitoneal injection of SeP purified protein significantly reduced insulin sensitivity and glucose tolerance in wildtype C57BL/6 mice. However, 71 patients with metabolic syndrome MetS had elevated levels of plasma SePP compared with 74 patients without metabolic syndrome 37 . The physiological concentration of SeP inhibits vascular endothelial growth factor-stimulated cell proliferation, tubule formation and migration.…”

Effects of Dietary Selenium Sources on Physiological Status of Laying Hens and Production of Selenium-Enriched Eggs

“…The antagonistic action of Se on heavy metals could attenuate the adverse effects of lead and cadmium on animal health (18)(19)(20)(21)(22). Long-term of Se supplementation gives out a potential therapeutic effect in subjects suffering from coronary artery disease (23). Se deficiency in food inhibits myocardial development and results in hypothyroidism, a weakened immune system, Ke-shan disease, and Kaschin-beck disease (4,24,25).…”

Effects of selenium supplementation on paraoxonase-1 and myeloperoxidase activity in subjects with cardiovascular disease: the Selenegene study, a double-blind randomized controlled trial

“…The absorbance was continuously monitored at 270 nm for 2 min. POX1 activity was expressed as millimoles of phenyl acetate hydrolyzed per minute [15].…”