Effects of selective oestrogen receptor modulators on proliferation in tissue cultures of pre- and postmenopausal human endometrium

Punyadeera, Chamindie; Kamps, Rick; Defrère, Sylvie; Dijcks, Fred A.; Goeij, A. de; Ederveen, A. G. H.; Dunselman, G.A.J.; Groothuis, Patrick G.

doi:10.1016/j.jsbmb.2008.09.002

Cited by 14 publications

(11 citation statements)

References 29 publications

(34 reference statements)

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“…We used PPT for these experiments to ensure that ligand binding was selective for ER; a similar but non-significant trend was obtained when E2 was used in the place of PPT (not shown). In line with expectations FRET signals with positive (pCY24) and negative (pECFP and pEYFP) control plasmids were ~30% and 0% respectively ( Figure 6 The human endometrium is an oestrogen target tissue and during the follicular phase of the cycle rising levels of circulating oestrogens increase the rate of endometrial cell proliferation [34] an observation that can be replicated using explant cultures of postmenopausal endometrium [35]. Studies in rodent models suggest that both proliferation and increased expression of progesterone receptor are mediated by ER [36].…”

Section: Fret Analysissupporting

confidence: 79%

Modulation of ERα transcriptional activity by the orphan nuclear receptor ERRβ and evidence for differential effects of long- and short-form splice variants☆

Bombail

Collins

Brown

et al. 2010

Molecular and Cellular Endocrinology

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Oestrogen receptor related proteins (ERRs) affect target gene expression without binding oestradiol. We investigated the functional activity of two splice variant isoforms of ERR beta (ERR beta S [short], ERR beta L [long]) expressed in human endometrium, where they are coexpressed with the oestrogen receptor alpha (ER alpha). Over-expression of ERRbetaL enhanced ER alpha-dependent ligand-induced activation of an ERE-luciferase reporter construct, altered the induction of c-myc mRNA and increased proliferation of Ishikawa cells whereas ERR beta S was found to reduce these endpoints. Fluorescent recovery after photobleaching (FRAP) revealed that intra-nuclear mobility of YFP-ERR beta S was more rapid than YFP-ERR beta L. Fluorescence resonance energy transfer (FRET) assays revealed a close association between ERR beta L and ER alpha following addition of ligand. We speculate that ERR beta L may alter ER alpha-dependent gene activation by enhancing the recruitment of co-activators. In conclusion, variant isoforms of ERR beta have differential effects on ER alpha-dependent gene expression and this has implications for human endometrial cell function.

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Section: Fret Analysissupporting

confidence: 79%

Modulation of ERα transcriptional activity by the orphan nuclear receptor ERRβ and evidence for differential effects of long- and short-form splice variants☆

Bombail

Collins

Brown

et al. 2010

Molecular and Cellular Endocrinology

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“…It acts as an antagonist both in the breast and in the endometrium, consistent with the present study. However, in contrast to our data, Punyadeera et al 25 found on the proliferative activity in glands of premenopausal endometrium tissue explants prepared from proliferative endometrium biopsies that raloxifene significantly enhanced the number of Ki-67Ypositive cells.…”

Section: Discussioncontrasting

confidence: 99%

Comparative Study of Tamoxifen and Raloxifene on Endometrial Cell Proliferation of Female Rats in Persistent Estrus

Rodrigues‐Junior

Santos

Costa

et al. 2012

Int J Gynecol Cancer

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“…For example, proliferation and apoptosis were strongly up-regulated in endometrium with estrogen treatment alone, while the vagina was weakly responsive to hormones. Increased proliferation in endometrium induced by estradiol treatment was also demonstrated in a study evaluating the effects of selective estrogen receptor modulators (SERM) on tissue explants from pre- and postmenopausal women by evaluating number of Ki67-positive cells [61]. This study found that whereas estradiol stimulated proliferation in premenopausal tissues by 55%, proliferation was increased by 250% in postmenopausal tissues.…”

Section: Effect Of Mht On the Immune Systemmentioning

confidence: 73%

The immune system in menopause: Pros and cons of hormone therapy

Ghosh

Rodriguez-García

Wira

2014

The Journal of Steroid Biochemistry and Molecular Biology

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With aging, a general decline in immune function is observed leading to immune-senescence. Several of these changes are gender specific affecting postmenopausal women. Menopause is a normal part of a woman’s lifecycle and consists of a series of body changes that can last from one to ten years. It is known that loss of sex hormones due to aging results in a reduction of immune functions. However, there remains a major gap in our understanding regarding the loss of immune functions particularly in the female reproductive tract (FRT) following menopause and the role of menopausal hormone therapy (MHT) in protecting against immune senescence. The current review presents an overview of changes in the immune system due to aging, focusing on genital tract immunity in menopausal women and the risks and benefits of using MHT.

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Effects of selective oestrogen receptor modulators on proliferation in tissue cultures of pre- and postmenopausal human endometrium

Cited by 14 publications

References 29 publications

Modulation of ERα transcriptional activity by the orphan nuclear receptor ERRβ and evidence for differential effects of long- and short-form splice variants☆

Modulation of ERα transcriptional activity by the orphan nuclear receptor ERRβ and evidence for differential effects of long- and short-form splice variants☆

Comparative Study of Tamoxifen and Raloxifene on Endometrial Cell Proliferation of Female Rats in Persistent Estrus

The immune system in menopause: Pros and cons of hormone therapy

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