Effects of Selected Lactobacillus plantarum as Probiotic on In vitro Ruminal Fermentation and Microbial Population

Wiryawa, Komang G.; Astuti, Wulansih Dwi; Wina, Elizabeth; Widyastuti, Yantyati; Suharti, Sri; Ridwan, Roni

doi:10.3923/pjn.2018.131.139

Cited by 23 publications

(24 citation statements)

References 27 publications

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“…However, postbiotic inclusion in post-weaning lambs did not affect total and individual VFA except for propionic acid. The finding was consistent with Astuti et al [26] who reported the use of L. plantarum as probiotics in in vitro rumen fermentation study showed no effect on total VFA production. The finding was also consistent with Qadis et al [27] who found no effect of probiotics from L. plantarum , Enterococcus faecium and Clostridium butyricum on total VFA in rumen fluid.…”

Section: Discussionsupporting

confidence: 92%

Effects of postbiotic supplementation on growth performance, ruminal fermentation and microbial profile, blood metabolite and GHR, IGF-1 and MCT-1 gene expression in post-weaning lambs

et al. 2019

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Background Postbiotics have been established as potential feed additive to be used in monogastric such as poultry and swine to enhance health and growth performance. However, information on the postbiotics as feed additive in ruminants is very limited. The aim of this study was to elucidate the effects of supplementation of postbiotics in newly-weaned lambs on growth performance, digestibility, rumen fermentation characteristics and microbial population, blood metabolite and expression of genes related to growth and volatile fatty acid transport across the rumen epithelium. Results Postbiotic supplementation increased weight gain, feed intake, nutrient intake and nutrient digestibility of the lambs. No effect on ruminal pH and total VFA, whereas butyrate and ruminal ammonia-N concentration were improved. The lambs fed with postbiotics had higher blood total protein, urea nitrogen and glucose. However, no difference was observed in blood triglycerides and cholesterol levels. Postbiotics increased the population of fibre degrading bacteria but decreased total protozoa and methanogens in rumen. Postbiotics increased the mRNA expression of hepatic IGF-1 and ruminal MCT-1. Conclusions The inclusion of postbiotics from L. plantarum RG14 in newly-weaned lambs improved growth performance, nutrient intake and nutrient digestibility reflected from better rumen fermentation and microbial parameters, blood metabolites and upregulation of growth and nutrient intake genes in the post-weaning lambs.

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Section: Discussionsupporting

confidence: 92%

Effects of postbiotic supplementation on growth performance, ruminal fermentation and microbial profile, blood metabolite and GHR, IGF-1 and MCT-1 gene expression in post-weaning lambs

et al. 2019

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“…The authors did not offer any discussion as to how a LAB strain could increase CH 4 production in vivo . Astuti et al (2018) evaluated 14 strains of L. plantarum in rumen in vitro experiments and identified strain U32 which had the lowest CH 4 production value when compared to the other LAB treatment groups. The authors hypothesized the addition of LAB may have stimulated the growth of lactic utilizing bacteria leading to increased production of propionic acid and a subsequent decrease in the hydrogen availability for methane production (Astuti et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Use of Lactic Acid Bacteria to Reduce Methane Production in Ruminants, a Critical Review

Doyle

Mbandlwa

Kelly³

et al. 2019

Front. Microbiol.

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Enteric fermentation in ruminants is the single largest anthropogenic source of agricultural methane and has a significant role in global warming. Consequently, innovative solutions to reduce methane emissions from livestock farming are required to ensure future sustainable food production. One possible approach is the use of lactic acid bacteria (LAB), Gram positive bacteria that produce lactic acid as a major end product of carbohydrate fermentation. LAB are natural inhabitants of the intestinal tract of mammals and are among the most important groups of microorganisms used in food fermentations. LAB can be readily isolated from ruminant animals and are currently used on-farm as direct-fed microbials (DFMs) and as silage inoculants. While it has been proposed that LAB can be used to reduce methane production in ruminant livestock, so far research has been limited, and convincing animal data to support the concept are lacking. This review has critically evaluated the current literature and provided a comprehensive analysis and summary of the potential use and mechanisms of LAB as a methane mitigation strategy. It is clear that although there are some promising results, more research is needed to identify whether the use of LAB can be an effective methane mitigation option for ruminant livestock.

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“…In fact, propionate and butyrate production is a pathway for hydrogen utilization and hydrogen is a major precursor to methane production ( Jeyanathan et al, 2014 ). Astuti et al (2018) also reported that the use of the U32 strain of L. plantarum reduced methane production and they reported that this decrease was probably due to the positive effect of LAB on the growth of LUB. The decrease in methanogen population may be due to the increase in F. succinogenes population as a result of microbial additives.…”

Section: Discussionmentioning

confidence: 96%

Effect of microbial feed additives on growth performance, microbial protein synthesis, and rumen microbial population in growing lambs

Direkvandi¹,

Mohammadabadi²,

Salem

2020

Translational Animal Science

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Arabi lambs (n =28; body weight = 24 ± 3.7 kg; average age = 120 ± 8 days) were used to investigate the effect of microbial additives on growth performance, microbial protein synthesis and rumen microbial population of fattening lamb based on completely randomized design. Four treatments were studied; (1) control (without additive; CON); (2) Lactobacillus fermentum and L. plantarum (FP); (3) Saccharomyces cerevisiae (SC) plus FP (SCFP) and (8) Megasphaera elsdenii plus SCFP (MSCFP). Lambs were inoculated before morning feeding (daily oral dosed) with a 50 mL microbial suspension as follow: FP, 50 mL bacterial suspension containing 4.5 × 10 8 colony-forming unit per day (cfu/day) of L. plantarum and L. fermentum (in ratio 50:50)); SCFP, 50 mL microbial suspension containing 4.5 × 10 8 cfu/day FP and 1.4 × 10 10 cfu/day SC; MSCFP, 50 mL microbial suspension containing 4.5 × 10 8 cfu/day Me, 4.5 × 10 8 cfu/day FP and 1.4 × 10 10 cfu/day SC. Feed intake and body weight of lambs were not affected by microbial additives. Average daily gain and feed efficiency were increased on day 0-21. The highest concentration of uric acid, total excreted purine derivatives (PD), microbial N, microbial CP and metabolizable protein were in MSCFP lambs. The ruminal population of Ruminococcus albus and Ruminococcus flavefaciens was higher in MSCFP and SCFP than CON and FP lambs. The highest and the lowest abundance of M. elsdenii and methanogen respectively was observed in lambs fed on microbial additives. The tendency to improve growth performance versus CON may be due to improvements in microbial protein synthesis and microbial populations, especially fiber-degrading bacteria. The decrease in the population of methanogens as a result of the use of microbial additives is another positive result.

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Effects of Selected Lactobacillus plantarum as Probiotic on In vitro Ruminal Fermentation and Microbial Population

Cited by 23 publications

References 27 publications

Effects of postbiotic supplementation on growth performance, ruminal fermentation and microbial profile, blood metabolite and GHR, IGF-1 and MCT-1 gene expression in post-weaning lambs

Effects of postbiotic supplementation on growth performance, ruminal fermentation and microbial profile, blood metabolite and GHR, IGF-1 and MCT-1 gene expression in post-weaning lambs

Use of Lactic Acid Bacteria to Reduce Methane Production in Ruminants, a Critical Review

Effect of microbial feed additives on growth performance, microbial protein synthesis, and rumen microbial population in growing lambs

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