Effects of selected electron transport chain inhibitors on 24-h hydrogen production by Synechocystis sp. PCC 6803

Burrows, Elizabeth H.; Chaplen, Frank; Ely, Roger L.

doi:10.1016/j.biortech.2010.10.042

Cited by 24 publications

(9 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hoxEFUYH operon is regulated by various environmental conditions, such as hydrogen, light, nitrate, nickel, oxygen and sulfur availabilities [ 25 ], in agreement with the finding that H 2 production can be increased in media with optimized concentrations of these elements [ 26 ]. The transcript levels of all hox genes are increased (five to sixfold) under microaerobic conditions [ 27 , 28 ], with an additional induction (10–12-fold) of hoxEF in darkness [ 27 ].…”

Section: Expression and Regulation Of The Genes Involved In Hydrogsupporting

confidence: 68%

Advances in the Function and Regulation of Hydrogenase in the Cyanobacterium Synechocystis PCC6803

Cassier‐Chauvat

Veaudor

Chauvat

2014

IJMS

View full text Add to dashboard Cite

In order to use cyanobacteria for the biological production of hydrogen, it is important to thoroughly study the function and the regulation of the hydrogen-production machine in order to better understand its role in the global cell metabolism and identify bottlenecks limiting H2 production. Most of the recent advances in our understanding of the bidirectional [Ni-Fe] hydrogenase (Hox) came from investigations performed in the widely-used model cyanobacterium Synechocystis PCC6803 where Hox is the sole enzyme capable of combining electrons with protons to produce H2 under specific conditions. Recent findings suggested that the Hox enzyme can receive electrons from not only NAD(P)H as usually shown, but also, or even preferentially, from ferredoxin. Furthermore, plasmid-encoded functions and glutathionylation (the formation of a mixed-disulfide between the cysteines residues of a protein and the cysteine residue of glutathione) are proposed as possible new players in the function and regulation of hydrogen production.

show abstract

Section: Expression and Regulation Of The Genes Involved In Hydrogsupporting

confidence: 68%

Advances in the Function and Regulation of Hydrogenase in the Cyanobacterium Synechocystis PCC6803

Cassier‐Chauvat

Veaudor

Chauvat

2014

IJMS

View full text Add to dashboard Cite

show abstract

“…The addition of 70 mmol/L fructose results in 3.7-fold increase in the initial hydrogen production rate from run without fructose. The 48 h dark anaerobic incubation of Synechocystis cells in HEPES buffer solution without fructose and N-deprivation condition in this work generated 0.927 µmol hydrogen from (7) (10 9 ) cells, which was acceptable when compared to 2.37 µmol hydrogen from (7) (10 9 ) cells shown in previous report (OD 730 =0.1 equal to (1.4)(10 8 ) cells/ mL) [5]. This minor difference can be explained by the difference of temperature, gas phase composition and growth phase of Synechocstis cells.…”

Section: Measurement Of Dry Cell Weightmentioning

confidence: 44%

“…Eliminating nitrate from medium constituents is established to be a key for dark hydrogen production, which is supported by an observation that a deletion mutant of the genes encoding both nitrate reductase and nitrite reductase significantly enhanced production [4]. Inhibitor addition to block of a certain electron sink pathway that is competitive to NiFe-hydrogenase is applied to increase hydrogen production [5].…”

Section: Introductionmentioning

confidence: 96%

Fructose-Mediated Elevation of Hydrogen Production in Glucose Tolerant Mutant of Synechocystis Sp. Strain PCC 6803 under the Dark Anaerobic Nitrate-Free Condition

Chongsuksantikul¹,

Asami²,

Yoshikawa³

et al. 2014

J Bioproces Biotech

View full text Add to dashboard Cite

Fructose is a potential additive that elevates a supply of electrons to hydrogenase for hydrogen production in cyanobacteria. A series of dark anaerobic hydrogen production experiments was performed to evaluate the validity of this assumption, in which fructose from 0 to 110 mmol/L was added to HEPES buffer solution on which a glucose tolerant mutant of unicellular cyanobacterium Synechocystis sp. strain PCC 6803 (GT strain) was incubated. Despite the reported knowledge that fructose was an inhibitor for photoheterotrophic growth of Synechocystis cells, GT strain assimilated fructose and represented a limited heterotrophic growth on fructose in HEPES buffer solution under dark anaerobic condition. The initial hydrogen production rate that was 0.025 mmol/L h in run without fructose increased to 0.0917 mmol/L h in run with 60-83 mmol/L fructose. The associated increase in the initial amount of endogenous glucose from 0.22 mmol/L in run without fructose to 0.36 mmol/L in run with 50 mmol/L fructose was observed. Fructose released the complete suppression of hydrogen production by nitrate. This work presents the first experimental evidence that cells of GT strain are able to assimilate fructose for cell growth in dark anaerobic condition. Our results show that hydrogen production in Synechocystis sp. strain can be significantly elevated by a proper addition of fructose to dark anaerobic HEPES buffer solution.

show abstract

“…Some reports showed that hydrogen consumption was reduced by knocking out the Hup which is one of the subunits of hydrogenase, such as Anabaena variabilis AVM13 ( ΔhupSL ), Nostoc punctiforme NHM5 ( ΔhupL ), Anabaena PCC 7120 AMC 414 ( ΔxisC ), Anabaena PCC 7120 ( ΔhupL , ΔhupL/ ΔhoxH , ΔhupW ), Nostoc PCC 7422 ( ΔhupL ) and Anabaena siamensis TISTR 8012 ( ΔhupS ) . Some metabolic processes can be inhibited by specific inhibitors, such as malonate, a competitive inhibitor of succinate dehydrogenase, which likely redirects the electron flow to nitrogenase and/or bidirectional Hox‐hydrogenase to enhance hydrogen production in the dark …”

Section: Genetic Engineering To Improve Hydrogen Productionmentioning

confidence: 99%

Microalgal Hydrogen Production

et al. 2020

View full text Add to dashboard Cite

Microalgae, as facultative aerobic organisms, convert solar energy to produce biohydrogen under anaerobic condition. Biohydrogen is a kind of ideal clean and renewable energy source with great commercial potential. Many endeavors have been focused on improving the biohydrogen yields by various means. Here, the research history of hydrogen production by microalgae (including cyanobacteria and green algae), the characteristics of nitrogenase and hydrogenase, the mechanisms of hydrogen production, the technological progress, and the application of enzymatic, genetic, and metabolic engineering methods to improve hydrogen production by microalgae are reviewed. In addition, the regulation of anaerobic metabolisms of Chlamydomonas reinhardtii after the disruption of key enzymes functioning in the fermentative pathways is discussed. Finally, the main challenges and obstacles facing the more efficient production and commercialization of hydrogen production from microalgae in the future are proposed.

show abstract

Effects of selected electron transport chain inhibitors on 24-h hydrogen production by Synechocystis sp. PCC 6803

Cited by 24 publications

References 47 publications

Advances in the Function and Regulation of Hydrogenase in the Cyanobacterium Synechocystis PCC6803

Advances in the Function and Regulation of Hydrogenase in the Cyanobacterium Synechocystis PCC6803

Fructose-Mediated Elevation of Hydrogen Production in Glucose Tolerant Mutant of Synechocystis Sp. Strain PCC 6803 under the Dark Anaerobic Nitrate-Free Condition

Microalgal Hydrogen Production

Contact Info

Product

Resources

About