Effects of Selaginella tamariscina on in vitro tumor cell growth, p53 expression, G1 arrest and in vivo gastric cell proliferation

Lee, In-Seon; Nishikawa, Akiyoshi; Furukawa, Fumio; Kasahara, Ken-ichiro; Kim, Soo-Un

doi:10.1016/s0304-3835(99)00202-5

Cited by 52 publications

(42 citation statements)

References 21 publications

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“…22,23) Chinese herbs including Sellaginella genus plants have been used as alternative anti-cancer agents in East Asia. 10,24) Hence, the present data provide a possible mechanistic basis for the anti-tumor or chemopreventive effect of Sellaginella extracts. The productions of excessive proinflammatory mediators, prostaglandins, and NO, through NF-kB pathway play an important role in severe inflammatory disease, 25) and thus the inhibition of proinflammatory mediators in inflammatory cells could beneficially suppress excessive inflammatory reaction.…”

Section: Discussionmentioning

confidence: 99%

“…Lee et al 10) reported that the water-extracted fraction of Selaginella tamariscina (Selaginellaceae) efficiently increased p53 gene expression and induced G1 arrest, suggesting that S. tamariscina is a candidate chemopreventive. Crude extracts of S. tamariscina also reduced the production of proinflammatory cytokines, interleukin-1b, and tumor necrosis factor-a in human mesangial cells.…”

Section: -3)mentioning

confidence: 99%

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Inhibition of Nuclear Factor-.KAPPA.B Activation by 2',8"-Biapigenin

Woo

Pokharel

Yang

et al. 2006

Biological & Pharmaceutical Bulletin

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Chemoprevention is considered to be one of the most promising strategies for the prevention of human cancers. It is defined as the use of either natural or synthetic compounds to block or retard the carcinogenic process, and many natural candidates including epigallocatechin, genistein and sulforaphane have been evaluated in terms of malignancy prevention. 1-3)Recently, it was suggested that chronic inflammation is associated with carcinogenesis. 4,5) Chronic inflammation leads to the induction of specific enzymes in affected tissues and cells. In particular, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) are responsible for the exaggerated production of NO and prostaglandins, respectively, and are believed to be involved in the pathogenesis of cancer.6,7) COX-2 expression increases during tumor progression in the stomach, suggesting that COX-2 participates in gastric tumorigenesis.8) It has also been reported that there is a strong positive relationship between the presence of iNOS and the frequency of mutation in colon tumor tissues. 9)Hence, the overproduction of prostaglandins and NO may act as both an endogenous initiator and as a promoter of carcinogenesis and specific inhibitors of COX-2 or iNOS might have applications as chemopreventive agents in human cancer. Lee et al. 10) reported that the water-extracted fraction of Selaginella tamariscina (Selaginellaceae) efficiently increased p53 gene expression and induced G1 arrest, suggesting that S. tamariscina is a candidate chemopreventive. Crude extracts of S. tamariscina also reduced the production of proinflammatory cytokines, interleukin-1b, and tumor necrosis factor-a in human mesangial cells.11) As a part of our program to screen for potential cancer chemopreventive compounds from medicinal plants, we isolated 2Ј,8Љ-biapigenin from S. tamariscina (Fig. 1). The biological activity of 2Ј,8Љ-biapigenin has not been studied.In the present study, we investigated the modulating effects of the bi-flavonoid, 2Ј,8Љ-biapigenin on the expressions and activities of iNOS and COX-2 induced by lipopolysaccharide (LPS) in Raw264.7 macrophage cells. We found that 2Ј,8Љ-biapigenin inhibited nuclear factor (NF)-kB activation, and that this action is required for its blocking effects on iNOS and COX-2. MATERIALS AND METHODS Extraction and Isolation of 2,8؆-Biapigenin WholeSelaginella tamariscina (600 g) was extracted with MeOH at room temperature to afford 50.5 g of residue. The methanol extract was suspended in water and then sequentially partitioned in dichloromethane, ethyl acetate, and n-butanol. Three grams of the EtOAc fraction was subjected to silica gel column chromatography using a CHCl 3 -MeOH-H 2 O (12 : 1 : 0.1→8 : 1 : 0.1→5 : 1 : 0.1→2 : 1 : 0.1→1 : 1 : 0.1→Me OH only) gradient elution system. Fractions were combined based on their TLC patterns to yield subfractions designated as E1-E10. Subfraction E4 (438.9 mg) was purified by Sephadex LH 20 column chromatography using MeOH : H 2 Oϭ2 : 1 as eluant to give four subfractions (E41-E44). ...

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Section: Discussionmentioning

confidence: 99%

Section: -3)mentioning

confidence: 99%

Inhibition of Nuclear Factor-.KAPPA.B Activation by 2',8"-Biapigenin

Woo

Pokharel

Yang

et al. 2006

Biological & Pharmaceutical Bulletin

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“…3d), Selaginella lepidophyla, Selaginella labordei, Selaginella moellendorffii, Selaginella delicatula, S. tamariscina, and Sellaginella doederleinii (Fig. 3e) were shown to have cytotoxic and antimutagenic effects due to the presence of bioflavonoids (Lee and Lin, 1988;Silva et al, 1995;Sun et al, 1997;Lee et al, 1999;Lin et al, 2000;Su et al, 2000;Chen JJ et al, 2005;Gayathri et al, 2005;Woo et al, 2005;Gao et al, 2007;Shi et al, 2008;Tan et al, 2009), while Pteris semipinnata (Fig. 4c) and P. multifida have cytotoxic effects due to the presence of diterpenes (Woerdenbag et al, 1996;Li et al, 1998Li et al, , 1999.…”

Section: Anti-cancer Antidiabetic and Antiviral Activitymentioning

confidence: 99%

“…show notable anti-cancer properties (Lai et al, 2010). The antithiamin substances in brackens, such as astragalin, isoquercitrin, rutin, caffeic acid, and tannic acid have Lee and Lin, 1988;Silva et al, 1995;Sun et al, 1997;Lee et al, 1999;Lin et al, 2000;Su et al, 2000;Chen et al, 2005b;Gao et al, 2007;Li J et al, 2014;Li S 2014;Elda et al, 2015 35 Guha et al, 1996 MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (Konoshima et al, 1996). A. evecta and Selaginella tamariscina have been shown to have significant antidiabetic activity (Miao et al, 1996;Nguyen, 2005).…”

Section: Anti-cancer Antidiabetic and Antiviral Activitymentioning

confidence: 99%

A review of the use of pteridophytes for treating human ailments

Xavier-ravi

Vigila

Zhang

et al. 2018

J. Zhejiang Univ. Sci. B

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Abstract:The aim of this review was to explore the pharmacological activity of early tracheophytes (pteridophytes) as an alternative medicine for treating human ailments. As the first vascular plants, pteridophytes (aka, ferns and fern allies) are an ancient lineage, and human beings have been exploring and using taxa from this lineage for over 2000 years because of their beneficial properties. We have documented the medicinal uses of pteridophytes belonging to thirty different families. The lycophyte Selaginella sp. was shown in earlier studies to have multiple pharmacological activity, such as antioxidant, anti-inflammatory, anti-cancer, antidiabetic, antiviral, antimicrobial, and anti-Alzheimer properties. Among all the pteridophytes examined, taxa from the Pteridaceae, Polypodiaceae, and Adiantaceae exhibited significant medicinal activity. Based on our review, many pteridophytes have properties that could be used in alternative medicine for treatment of various human illnesses. Biotechnological tools can be used to preserve and even improve their bioactive molecules for the preparation of medicines against illness. Even though several studies have reported medicinal uses of ferns, the possible bioactive compounds of several pteridophytes have not been identified. Furthermore, their optimal dosage level and treatment strategies still need to be determined. Finally, the future direction of pteridophyte research is discussed.

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“…Alteration of the cell DNA content, histogram measurements of hypodiploid DNA formation was carried out by flow cytometer using EPICS ™ cytometer-Beckman Coulter [10].…”

Section: Cell Cycle Analysismentioning

confidence: 99%

Apoptosis Induction by Cestrum parqui L’Hér. leaves on HL-60 Cell Line: Identification of Active Phytomolecules

Chenni¹,

Torres²,

Estévez³

et al. 2015

IJCR

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IntroductionCancer, one of the leading causes of human death, has attracted more attention due to the complexity of etiology, diagnosis and treatment [1]. In recent years, chemotherapeutic management against various types of cancer including leukemia has been increased rapidly though some times cancer cells resistance against chemotherapy [2,3]. In this respect, search for effective anticancer agent that inhibit the proliferation of the cells as well as inhibit the resistance nature of the cancer cells and induce differentiation of the monocytes is an ongoing challenge. Therefore to find out anticancer agent with multi-target efficacies, attention has been focused on plant products as the plant products have been used as remedies for several human diseases including cancer from ancient times with lower side effects [4]. The reasons for using them that they contain variety of chemical compounds usually secondary metabolites with therapeutic values [5]. Cestrum parqui is belonging to the family Solanaceae, usually known as "green or Chilean cestrum" in Tunisia, are rich in various secondary metabolites such as saponins, polyhydroxylated terpenes (C13 norisoprenoids, sesquiterpenes, spirostanes and pseudosapogenins) and ent-kaurene glycosides (parquin and carboxyparquin) [6][7][8]. Several scientific reports demonstrate that C. parqui exhibits a wide spectrum of pharmacological activity when administered systemically or in isolated organ preparations. For example, it is used to treat headache, rheumatic pain, scabies, dermatosis, diarrhaea and metrorraghia [7]. The plant is also used in Chilean folk Abstract Background: In our time, there is an urgent need to discover natural anticancer products without toxic side's effects. Objective: The present study was conducted to investigate the anticancer activity of the methanolic extract of Cestrum parqui leaves as well as identification of the active compounds in this concern. Materials and Methods: Eight column chromatographic fractions were tested against human leukaemia cell line (HL-60) and the cell viability was measured by dimethylthiazol tetrazolium assay. Studies of apoptosis, cell cycle and DNA fragmentation were carried out following the standard protocol. Effective fractions were analysed by high performance liquid chromatography-ultra violet, HPLC-evaporative light scattering detector and preparative-HPLC. Finally the active compounds were identified by gas chromatography coupled to mass spectrometry. Results: Out of eight, fraction II showed remarkable inhibition on the growth of HL-60 cell line and fraction I showed moderate activity on such inhibition. Mechanisms of the inhibition were identified as cell cycle arrestation and apoptosis induction by the said fractions. Chromatographic and mass spectrometric analysis of the fraction II indicated the presence of oleanolic and ursolic acids as biologically active compounds. Conclusion: This study is the first investigation that shows Cestrum parqui has anticancer activity and the responsible compounds are olea...

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Effects of Selaginella tamariscina on in vitro tumor cell growth, p53 expression, G1 arrest and in vivo gastric cell proliferation

Cited by 52 publications

References 21 publications

Inhibition of Nuclear Factor-.KAPPA.B Activation by 2',8"-Biapigenin

Inhibition of Nuclear Factor-.KAPPA.B Activation by 2',8"-Biapigenin

A review of the use of pteridophytes for treating human ailments

Apoptosis Induction by Cestrum parqui L’Hér. leaves on HL-60 Cell Line: Identification of Active Phytomolecules

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