Effects of seedborne potato virus Y on Russet Norkotah performance

Rykbost, K. A.; Hane, D. C.; Hamm, Philip B.; Voss, Ronald; Kirby, Donald W.

doi:10.1007/bf02855205

Cited by 25 publications

(18 citation statements)

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“…These symptomless carriers are also good sources of virus that is spread to neighboring crops. Tolerant cultivars infected with PVY and expressing mild or transient symptoms have reduced yields, and virus is readily acquired from such plants by aphids and spread among other susceptible cultivars (39,77,78,91). Many cultivars are included in the "tolerant" category (http://oregonstate.edu/potatoes/latent to PVY list.htm), but cultivars Shepody and Russet Norkotah are two of the most problematic, and they are widely grown, accounting for over 12 and 5% of the total U.S. and Canadian seed acreage, respectively, in 2009.…”

Section: How Politics Affects Pvy Distribution and By Extension The Dmentioning

confidence: 99%

Potato virus Y: An Evolving Concern for Potato Crops in the United States and Canada

et al. 2010

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Section: How Politics Affects Pvy Distribution and By Extension The Dmentioning

confidence: 99%

Potato virus Y: An Evolving Concern for Potato Crops in the United States and Canada

et al. 2010

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“…2 Accurate measures of virus levels are important for commercial potato growers as well. Increased levels of seed borne PVY have been shown to have a direct negative effect on potato yields (Gladders and Campbell-Hill 1988;Kurppa and Hassi 1989;Mondjana et al 1993;Hane and Hamm 1999;Rykbost et al 1999). More recently Nolte et al (2003) estimated that for every percent increase in seed borne PVY commercial yields fall by 1.4-1.75 hundredweight (cwt) per acre.…”

Section: Introductionmentioning

confidence: 97%

Statistical Analysis of the Laboratory Methods Used to Detect Potato Virus Y

2009

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According to the rules of certification for seed potatoes in Idaho, the maximum allowed content of potato virus Y (PVY) in seed potatoes is 2%. A number of laboratory methods are available to test for the presence of PVY. These methods are likely to have different degrees of accuracy. Consequently, the choice of a method used to detect PVY is likely to affect the test outcome and therefore economic benefits received by seed potato growers. This paper presents a statistical analysis of PVY detection performance of a traditional ELISA-based method versus two new PCR-based methods. The latter are considered to possess superior characteristics as compared to ELISA. We find that PVY detection performance of one of the PCRbased methods is statistically different from PVY detection performance of ELISA. Furthermore, this PCR-based method is less likely to provide a PVY positive outcome.However, PVY detection performance of another PCRbased method is not statistically different from ELISA. Finally, we find that a type of sample material tested affects PVY detection outcome. A positive PVY outcome is less likely if potato tubers are tested and it is more likely if potato leaves are tested.Resumen En Idaho, de acuerdo con las reglas de certificación para semilla de papa, el contenido máximo permitido de virus Y (PVY) en semilla es 2%. Existen varios métodos de laboratorio para probar la presencia de PVY. Estos métodos pueden tener distintos grados de precisión. En consecuencia la elección de un método para detectar PVY puede afectar el resultado de la prueba y por lo tanto los beneficios recibidos por los cultivadores de semilla de papa. Este artículo presenta un análisis estadístico del rendimiento de detección de PVY de un método tradicional basado en ELISA versus dos métodos nuevos basados en PCR. Los últimos son considerados que tienen características superiores en comparación a la prueba de ELISA. Encontramos que el rendimiento de detección de PVY de uno de los métodos basados en PCR es estadísticamente diferente de lo encontrado en la detección de PVY por ELISA. Más aún, este método basado en PCR parece proporcionar menores resultados positivos para PVY. Sin embargo, el rendimiento de detección de PVY con el otro método basado en PCR no es estadísticamente diferente al de ELISA. Finalmente, encontramos que un tipo de material probado afecta el resultado de la detección. Un resultado positivo para PVY es menos probable en tubér-culos de papa y es más probable cuando hojas de la planta de papa son probadas.

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“…The vegetative propagation of potato enables systemic viruses to persist from 1 year to the next resulting in an overall decline in productivity. Depending on the cultivar, time of infection, and environment, yield losses associated with PVY range from 10-80% (deBokx and Huttinga 1981; Rykbost et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Validation and Implementation of Marker-Assisted Selection (MAS) for PVY Resistance (Ry adg gene) in a Tetraploid Potato Breeding Program

et al. 2009

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The gene Ry adg from S. tuberosum ssp. andigena provides extreme resistance to PVY. This gene has been genetically mapped to chromosome XI and linked PCRbased DNA markers have been identified. Advanced tetraploid russeted potato clones developed by the U.S. Pacific Northwest Potato Breeding ('Tri-State') Program with Ry adg PVY resistance were used in this study. The objective of this work was to assess the usefulness of molecular markers linked to Ry adg as a tool for selecting PVY resistance in a tetraploid potato breeding program. To achieve this, a full-sib tetraploid population segregating for Ry adg was screened with molecular markers linked to Ry adg , artificially inoculated with PVY O and evaluated in the greenhouse. A large percentage (96.4%) of the segregating lines showed coincidence between molecular markers and ELISA results at 40 days after inoculation. This justifies the use of molecular markers as an alternative to artificial inoculation followed by ELISA. Segregation (resistant vs. susceptible) based on ELISA and molecular marker results in the full-sib population indicated the presence of Ry adg as a simplex in the PVY resistant parent OR00030-1. Additional full-sib populations segregating for the Ry adg gene coming from OR00030-1 and from a related clone, AOR00628-3, were evaluated under field conditions. MAS can be used as a fast and efficient tool to select for PVY resistance, reducing the number of PVY susceptible lines retained for succeeding field evaluations, and thereby increasing the odds of generating PVY resistant potato varieties.Resumen El gen Ry adg de S. tuberosum ssp. andígena ofrece resistencia extrema contra el virus Y de la papa (PVY). Este gen ha sido genéticamente mapeado en el cromosoma XI y se han identificado marcadores ligados de ADN basado en PCR (reacción en cadena de la polimerasa). Clones avanzados tetraploides de papa rugosas, desarrollados por el Programa de Mejoramiento del Pacífico Noroeste de los Estados Unidos (`Tri-State´) con resistencia Ry adg al PVY fueron utilizados en este estudio. El objetivo de este trabajo fue evaluar la utilidad de los marcadores moleculares ligados a Ry adg , como una herramienta para seleccionar resistencia al PVY en un programa de mejoramiento de papa tetraploide. Para lograr esto, una población tetraploide de hermanos completos, segregantes para Ry adg fue tamizada mediante marcadores moleculares ligados al Ry adg , inoculada artificialmente con PVY o y Am. evaluada en invernadero. Un alto porcentaje (96.4%) de las líneas segregantes mostró coincidencia entre los marcadores moleculares y los resultados de ELISA 40 días después de la inoculación. Esto justifica el uso de marcadores moleculares como una alternativa a la inoculación artificial seguida por ELISA. La segregación (resistente vs. susceptible) basada en ELISA y los resultados de los marcadores moleculares en la población de hermanos completos, indicó la presencia de Ry adg como un simplex en el progenitor PVY resistente OR00030-1. Poblaciones adicionales de hermanos comp...

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Effects of seedborne potato virus Y on Russet Norkotah performance

Cited by 25 publications

References 1 publication

Potato virus Y: An Evolving Concern for Potato Crops in the United States and Canada

Potato virus Y: An Evolving Concern for Potato Crops in the United States and Canada

Statistical Analysis of the Laboratory Methods Used to Detect Potato Virus Y

Validation and Implementation of Marker-Assisted Selection (MAS) for PVY Resistance (Ry adg gene) in a Tetraploid Potato Breeding Program

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