Effects of seawater alkalinity on calcium and acid–base regulation in juvenile European lobster ( Homarus gammarus ) during a moult cycle

Middlemiss, Karen L.; Urbina, Mauricio A.; Wilson, Rod W.

doi:10.1016/j.cbpa.2015.12.002

Cited by 10 publications

(6 citation statements)

References 41 publications

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“…In the absence of sufficient macronutrients, cannibalism in H. gammarus may supplement exoskeletal development and mineralization (a process which is critical to growth and survival). Recently, water with a low alkalinity in larviculture systems has been suggested to explain cannibalism in H. gammarus (Middlemiss, Urbina & Wilson, ). Finally, it appears from our results that nutritional requirements may be specific to both species and developmental stage, even in closely related species (i.e.…”

Section: Discussionmentioning

confidence: 99%

Comparative survival and growth performance of European lobster larvae,Homarus gammarus, reared on dry feed and conspecifics

et al. 2017

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A bottleneck of crustacean larval culture concerns nutrition and associated cannibalism in communal rearing systems, which impact on larval survival, development and growth. For early‐stage European lobster, Homarus gammarus larvae, feeding ecology and body composition are largely unknown. We initiated four progressive feeding experiments (novel feed types, feeding regime and feed size and cannibalism effects) on growth and survival, to inform and update husbandry protocols. Performance of larvae offered a dry commercial feed was not significantly different compared with a conventional wet plankton feed of the same ration and size grade (both within 600–1,000 μm). Further experiments found that the same ration of dry feed offered six times daily improved development and growth, over the conventional regime of three times daily. Small‐grade dry feed (particles: 250–360 μm) improved larval performance compared with a larger feed (360–650 μm). Larvae were also fed different proportions of dry feed and/or conspecifics in both communal and individual rearing systems (the latter preventing cannibalism via segregation). Individually reared larvae, fed only dead conspecifics, displayed the greatest survival (80%) to postlarvae. This underlines the impact of cannibalism on survival and nutrition in H. gammarus larviculture. A final experiment analysed H. gammarus zoea 1 composition, identifying deficiencies in ash and carbohydrate in lobster feeds. This suggests a need for a species‐specific, formulated dry feed for H. gammarus larviculture. Our research represents the first investigation of H. gammarus larval composition and dietary requirements and highlights decreased growth potential associated with providing nutrition solely from generic commercial feed.

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Section: Discussionmentioning

confidence: 99%

Comparative survival and growth performance of European lobster larvae,Homarus gammarus, reared on dry feed and conspecifics

et al. 2017

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“…Seawater samples for measuring TA were taken at the beginning and end of the ∼135 min flux period, preserved by adding 40 µl of 4% (w/v) mercuric chloride per 10 ml of seawater, and stored at 4°C ( Dickson et al, 2007 ) prior to analysis by double titration using an autotitrator (Metrohm 907 Titrando with 815 Robotic USB Sample Processor XL, Metrohm). TA measurements were made using a double titration method modified from Cooper et al (2010) as detailed by Middlemiss et al (2016) . Briefly, 20 ml samples were titrated to pH 3.89 using 0.02 mol l −1 HCl whilst gassing with CO 2 -free N 2 ; pH was then returned to starting values by titrating with 0.02 mol l −1 NaOH.…”

Section: Methodsmentioning

confidence: 99%

Rapid blood acid–base regulation by European sea bass (Dicentrarchus labrax) in response to sudden exposure to high environmental CO2

Montgomery

Kwan

Davison

et al. 2022

Journal of Experimental Biology

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Fish in coastal ecosystems can be exposed to acute variations in CO2 of between 0.2-1 kPa CO2 (2,000 - 10,000 µatm). Coping with this environmental challenge will depend on the ability to rapidly compensate the internal acid-base disturbance caused by sudden exposure to high environmental CO2 (blood and tissue acidosis); however, studies about the speed of acid-base regulatory responses in marine fish are scarce. We observed that upon sudden exposure to ∼1 kPa CO2, European sea bass (Dicentrarchus labrax) completely regulate erythrocyte intracellular pH within ∼40 minutes, thus restoring haemoglobin-O2 affinity to pre-exposure levels. Moreover, blood pH returned to normal levels within ∼2 hours, which is one of the fastest acid-base recoveries documented in any fish. This was achieved via a large upregulation of net acid excretion and accumulation of HCO3− in blood, which increased from ∼4 to ∼22 mM. While the abundance and intracellular localisation of gill Na+/K+-ATPase (NKA) and Na+/H+ exchanger 3 (NHE3) remained unchanged, the apical surface area of acid-excreting gill ionocytes doubled. This constitutes a novel mechanism for rapidly increasing acid excretion during sudden blood acidosis. Rapid acid-base regulation was completely prevented when the same high CO2 exposure occurred in seawater with experimentally reduced HCO3− and pH, likely because reduced environmental pH inhibited gill H+ excretion via NHE3. The rapid and robust acid-base regulatory responses identified will enable European sea bass to maintain physiological performance during large and sudden CO2 fluctuations that naturally occur in coastal environments.

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“…The common occurrence of low alkalinity in intensive aquaculture systems may slow post‐moult calcification in juvenile lobster ( Homarus gammarus ), increasing the risk of mortality through cannibalism (Middlemiss et al . 2016). The requirements for alkalinity in crustacean culture are easy to meet in alkaline regions.…”

Section: Resultsmentioning

confidence: 99%

Planktonic bacteria in white shrimp ( Litopenaeus vannamei ) and channel catfish ( Letalurus punetaus ) aquaculture ponds in a salt‐alkaline region

Zhao

Guan

et al. 2021

Letters Applied Microbiology

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Aquaculture in salt‐alkaline regions is encouraged in China, and culture of many aquatic species has been introduced into these areas. In this study, we cultured two species, white shrimp (Litopenaeus vannamei) and channel catfish (Letalurus punetaus) separately in aquaculture ponds in a salt‐alkaline region in northwest China and assessed the impacts of the aquaculture operations on the planktonic bacterial community in the culture ponds. Culture of both species decreased the planktonic bacterial diversity and altered the bacterial community structure in the aquaculture ponds compared with the source water. Among the 10 dominant bacterial phyla, 8 were significantly correlated with environmental parameters; the exception was Actinobacteriota, the most dominant phylum, and Firmicutes. Proteobacteria and Bacteroidota abundances showed significant positive correlations with alkalinity, whereas Patescibacteria, Cyanobacteria, Planctomycetota, and Verrucomicrobiota abundance were positively correlated with salinity. Linear regression analysis showed that alkalinity was positively correlated with bacterial beta diversity and salinity was negatively correlated with that. In addition, white shrimp aquaculture significantly lowered the alkalinity, which suggests that culture of this species in inland salt‐alkaline regions is a potential dealkalization solution.

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Effects of seawater alkalinity on calcium and acid–base regulation in juvenile European lobster ( Homarus gammarus ) during a moult cycle

Abstract: 27Fluxes of NH4 + (acid) and HCO3 -(base), and whole body calcium content were

Cited by 10 publications

References 41 publications

Comparative survival and growth performance of European lobster larvae,Homarus gammarus, reared on dry feed and conspecifics

Comparative survival and growth performance of European lobster larvae,Homarus gammarus, reared on dry feed and conspecifics

Rapid blood acid–base regulation by European sea bass (Dicentrarchus labrax) in response to sudden exposure to high environmental CO2

Planktonic bacteria in white shrimp ( Litopenaeus vannamei ) and channel catfish ( Letalurus punetaus ) aquaculture ponds in a salt‐alkaline region

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