Effects of TNF‐α blocking on experimental periodontitis and type 2 diabetes in obese diabetic Zucker rats

Abstract

In this study anti-TNF-α treatment improved glucose tolerance and compensated for the increased periodontal disease in obese diabetic Zucker. PD did not influence diabetic parameters assessed including complications of the rats kidneys.

Ligature‐associated bacterial profiles are linked to type 2 diabetes mellitus in a rat model and influenced by antibody treatment against TNF‐α or RAGE

“…In brief, periodontal bone support in the obese groups (T2D) was decreased except for the OPDE group which could not be separated from the lean LPD group (non T2D). (Grauballe et al, 2015). Treatment with anti‐RAGE had no influence on periodontal support, compared to that of the control group (Grauballe et al, 2016).…”

“…All data concerning anti‐TNF‐α (Grauballe et al, 2015) and anti‐RAGE (Grauballe et al, 2016) treatment on periodontal and glycemic status has been presented elsewhere. In brief, periodontal bone support in the obese groups (T2D) was decreased except for the OPDE group which could not be separated from the lean LPD group (non T2D).…”

“…Treatment with anti‐RAGE had no influence on periodontal support, compared to that of the control group (Grauballe et al, 2016). Furthermore, anti‐TNF‐α treatment significantly improved the insulin resistance (Grauballe et al, 2015), and anti‐RAGE significantly improved both glucose tolerance and insulin resistance compared to the control group (Grauballe et al, 2016). …”

“…The present study was undertaken as part of a group of studies on experimental PD and T2D in rats, the additional results are presented elsewhere (Grauballe, Ostergaard, Schou, Flyvbjerg, & Holmstrup, 2015; Grauballe, Ostergaard, Schou, Flyvbjerg, & Holmstrup, 2016). …”

“…At week 4, food and water consumption and urine and feces production were measured in 12 randomly selected rats from each group, which were placed in metabolic cages (TECNIPLAST, Buguggiate, Italy) for 20 h. At week 5, final OGTTs were performed and at the end of week 5, all rats were euthanized under general anaesthesia with pentobarbital (Glostrup Apotek, Glostrup, Denmark) (100 mg/kg intra‐peritoneal) (Grauballe et al, 2015). Rats were decapitated and ligatures were removed with sterile forceps and both ligatures from each rat were put into one sterile tube containing MagNa pure bacteria lysis buffer (Roche, Mannheim, Germany) and stored at −80°C until DNA isolation.…”