Effects of <scp>d</scp>‐valine on periodontal or peri‐implant pathogens: Porphyromonas gingivalis biofilm

Hua, Qi; Li, Baosheng; Wang, Heling; Cai, Qing; Xu, Quan; Yunxia, Cui; Meng, Weiyan

doi:10.1002/jper.17-0405

Cited by 21 publications

(17 citation statements)

References 38 publications

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“…Then, the cultures were inoculated into fresh BHI broth supplemented by menadione and hemin. As previously reported, the biofilm formed by bacterial colonies at 10 8 CFU/mL exhibited a tightly connected and homogeneous structure [25]. Consequently, for all assays in the present study, bacteria in Journal of Oral Science, Vol.…”

Section: Bacterial Culture and Reagentssupporting

confidence: 80%

“…Such contrary effects may be associated with the different experimental conditions and bacterial strains because the specific effects of d-amino acid are not similar for all types of bacterium. However, the results of a previous study revealed the potential role of the antibacterial effect on P. gingivalis [25]. On the basis of these extended preliminary experiments, d-arginine was selected as the target amino acid, considering that the arginine residue may have a potential role in antimicrobial property [40].…”

Section: Discussionmentioning

confidence: 99%

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Effects of D-arginine on Porphyromonas gingivalis biofilm

Liu

et al. 2020

J Oral Sci

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Porphyromonas gingivalis (P. gingivalis) is one of the major pathogenic bacteria of periodontitis or peri-implantitis. P. gingivalis tends to attach to the implant's neck with the formation of biofilm, leading to peri-implantitis. d-arginine has been shown to have a potential antimicrobial role. In this study, P. gingivalis was cultured in Brain Heart Infusion broth together with d-arginine. After 3 days (inhibition) or 6 days (dissociation), these were characterized using crystal violet (CV) staining for the biofilm, extracellular polysaccharide (EPS) production from the biofilm, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay for biofilm activation. Furthermore, the P. gingivalis biofilm was observed by scanning electron microscopy (SEM). d-arginine effectively reduced biomass accumulation and promoted dissociation at concentrations of ≥50 mM and 100 mM, respectively. Through CV staining, d-arginine concentrations of EPS production from the biofilm for inhibition and dissociation effects was ≥50 mM and 100 mM, respectively. In addition, d-arginine affected biofilm activation for the corresponding concentrations: ≥60 mM for inhibition and ≥90 mM for dispersal. Under SEM observation, d-arginine changed the P. gingivalis biofilm structure in relatively high concentrations for inhibition or dissociation, respectively. The authors concluded that d-arginine could inhibit the formation of P. gingivalis biofilm and promote the dissociation of P. gingivalis biofilm.

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Section: Bacterial Culture and Reagentssupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Effects of D-arginine on Porphyromonas gingivalis biofilm

Liu

et al. 2020

J Oral Sci

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“…Periodontal and peri‐implant disease are inflammatory conditions associated with the soft (gingivae) and hard (alveolar bone) tissues surrounding teeth and dental implants, respectively. The classical risk factors of periodontal (gingivitis and periodontitis) and peri‐implant (peri‐implant mucositis and peri‐implantitis) diseases include bacterial overload in the oral biofilm and an immunocompromised health status (as observed in patients with acquired immune deficiency syndrome and individuals infected with the human immunodeficiency virus [HIV]) . If left uncontrolled/untreated, periodontal and peri‐implant diseases may result in tooth loss and failure of osseointegration, respectively .…”

Section: Introductionmentioning

confidence: 99%

“…The classical risk factors of periodontal (gingivitis and periodontitis) and peri-implant (peri-implant mucositis and periimplantitis) diseases include bacterial overload in the oral biofilm and an immunocompromised health status (as observed in patients with acquired immune deficiency syndrome and individuals infected with the human immunodeficiency virus [HIV]). [3][4][5][6][7] If left uncontrolled/ untreated, periodontal and peri-implant diseases may result in tooth loss and failure of osseointegration, respectively. 8,9 Although colonization and proliferation of anaerobic gram-negative bacteria in the oral biofilm is often associated with the onset and progression of periodontal and peri-implant diseases, [10][11][12][13] studies [14][15][16][17][18][19][20] have shown that viruses, such as Epstein-Barr virus (EBV), human cytomegalovirus (HCMV), and herpes simplex virus (HSV), have also been detected in subgingival oral biofilm samples of patients with periodontal and peri-implant diseases.…”

Section: Introductionmentioning

confidence: 99%

Contribution of herpesviruses in the progression of periodontal and peri‐implant diseases in systemically healthy individuals

Binshabaib

ALHarthi

Salehpoor

et al. 2018

Reviews in Medical Virology

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The aim of this review was to assess the contribution of herpesviruses in the subgingival oral biofilm in the progression of periodontal and peri-implant diseases in systemically healthy individuals. The literature review was customized to summarize the pertinent information for the following reasons: (1) A systematic review regarding the role of herpesviruses in the etiopathogenesis of periodontal disease has recently been published; and (2) a limited number of studies have assessed the association of herpesviruses with peri-implant diseases. To date, five observational studies have assessed the presence of herpesviruses in the subgingival oral biofilm of individuals with peri-implant diseases. In these studies, dental implants were in place for up to approximately 8 years. In two studies, human cytomegalovirus (HCMV) was more often isolated from the peri-implant sulci of sites with than without peri-implantitis. In one study, a low prevalence of HCMV compared with Epstein-Barr virus (EBV) was associated with the progression of peri-implantitis. In previous studies, the presence of EBV in the subgingival oral biofilm was associated with the onset of peri-implantitis and peri-implant mucositis, respectively. Major limitations of the studies assessed were the absence of blinding and lack of power analysis for sample size estimation. In conclusion, the presence of herpesviruses in the periodontal and peri-implant subgingival oral biofilm is an indicator of periodontal and peri-implant diseases in systemically healthy individuals; however, further studies with a statistically justified sample-size are needed to understand and refine this association.

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“…We previously reported that D-arginine and D-valine were effective in inhibiting P. gingivalis bio lm formation and promoting the disassembly of mature P. gingivalis bio lm respectively 15,16 . Considering that different D-AAs might be involved in different metabolic forms of bacteria, we speculated that combined application of D-AAs would present synergistic effects on bio lm.…”

Section: Discussionmentioning

confidence: 99%

Synergistic effects of D-arginine, D-methionine and D-histidine on the inhibition and eradication of Porphyromonas gingivalis biofilm

Zhang

Cai

et al. 2020

Preprint

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Porphyromonas gingivalis (P. gingivalis) biofilm is involved in peri-implantitis and periodontitis. Whether a mixture of D-AAs has synergistic effects on P. gingivalis biofilm remains unknown. The aim of this study was to investigate the effects of multiple D-AAs on P. gingivalis biofilm. D-arginine (R), D-methionine (M) and D-histidine (H) were used. The bacterial growth activity and minimum inhibitory concentrations (MICs) were determined. The effects of D-AAs mixture on biofilm biomass, extracellular polymeric substances (EPS), biofilm morphology, cytotoxicity, biofilm structure and bacterial membrane integrity were determined. D-AAs mixture delayed the proliferation of P. gingivalis below MICs. Mixtures of D-AAs displayed definite effects on the inhibition and disassembly of biofilm than single D-AAs. The EPS content increased with D-AAs concentrations. D-AAs mixture damaged the integrity of biofilm, while RMH showed the best effects. 8 mM RH and 4 mM RMH had no cytotoxicity. 4 mM RMH decreased biofilm thickness and altered bacteria membranes structure. Hence, the combined mixture of 4 mM RMH presents the best synergistic effects on the inhibition and eradication of P. gingivalis biofilm. Our study provides new insights of application of D-AAs for the treatment of peri-implantitis and periodontitis.

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Effects of d‐valine on periodontal or peri‐implant pathogens: Porphyromonas gingivalis biofilm

Abstract: Background: When presented with a surface or an interface, bacteria often grow as

Cited by 21 publications

References 38 publications

Effects of D-arginine on <i>Porphyromonas gingivalis</i> biofilm

Effects of D-arginine on <i>Porphyromonas gingivalis</i> biofilm

Contribution of herpesviruses in the progression of periodontal and peri‐implant diseases in systemically healthy individuals

Synergistic effects of D-arginine, D-methionine and D-histidine on the inhibition and eradication of Porphyromonas gingivalis biofilm

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