In this study, the informative bounds of neural networks (NN) prediction with respect to the utilization of less fatigue data for fatigue life assessment of composite material covering a wide range of stress ratios R was examined and investigated. Fiberglass reinforced polyester of [90/0/±45/0]S lay-up with fatigue data of various stress ratios (R = 0.1, 0.5, 0.7, 0.8, 0.9, -0.5, -1, -2 and 10) was examined in the present paper. Multi-layer Perceptrons (MLP) trained with Levenberg-Marquardt algorithm was utilized to result in fast and efficient NN model and Bayesian regularization technique was incorporated to deal with limited training data chosen for the model. The developed NN model was trained with fatigue data from only two stress ratios, where three sets of two stress ratio values were formed and used as the training sets, namely R = 0.1 and 0.5, R = 0.1 and -1, and R = 0.1 and 10, respectively. It was obtained that fatigue data from R = 10 produced the widest bounds of prediction, namely having the highest estimated standard deviation value from the fatigue lives predicted. Furthermore, it is revealed in the current study knowing the fact that fatigue data from R = 10 have the highest estimated standard deviation and subsequently including the fatigue data as one of the training data set, the NN model trained could produce the lowest mean squared error (MSE) value for the results of fatigue life prediction. This is justifying also the selection of training set of R = 0.1 and 10 as best training set in the previous study, which is based on the stress ratios' better relative positions in the corresponding constant life diagram (CLD). Finally, taking the highest estimated standard deviation value from fatigue data of R = 10 as the conservative estimated bounds of NN prediction, it was shown that for the NN prediction of fatigue life whose noticeable discrepancies with the experimental data, the discrepancies were well confined within the conservative bounds of prediction.