Effects of Sample Handling and Analytical Procedures on Thyroid Hormone Concentrations in Pregnant Women’s Plasma

Villanger, Gro Dehli; Learner, Emily R; Longnecker, Matthew P.; Ask, Helga; Aase, Heidi; Knudsen, Gun Peggy; Reichborn‐Kjennerud, Ted; Engel, Stephanie M.

doi:10.1097/ede.0000000000000606

Cited by 12 publications

(8 citation statements)

References 27 publications

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“…laboratory. Study with similar results by Gro et al 16 conducted in Norway in 2017 showed that various handling conditions have no effect on TSH in plasma kept for up to 72-hours at room temperature. Recently Gang et al reported that although interpreting results of thyroid function tests becomes straight-forward but, it needs to be corelated with pre-analytical and post-analytical variables in order to improve efficacy and ignorance to these factors may lead to complications in patient's health 17 .…”

Section: S105supporting

confidence: 62%

Effect of Preanalytical Variables on Serum Thyroid Stimulating Hormone Analysis by Chemiluminescence Method

Rehman

Asif

Malik

et al. 2021

PAFMJ

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Objective: To identify effect of pre-analytical variables on serum thyroid stimulating hormone. Study Design: Cross sectional study. Place and Duration of Study: Armed Forces Institute of Pathology (AFIP) Rawalpindi, Department of Chemical Pathology & Endocrinology, from Mar 2018 to Aug 2018. Methodology: Hundred subjects with ages ranging from 18 to 34 years, irrespective of gender, were randomly selected for this study. Five milliliters venous blood sample was collected from each subject in a serum separator and divided into two aliquots. First aliquot was centrifuged and analyzed immediately for TSH, while second aliquot was stored for 24 hours and was then analyzed. TSH was measured by third generation assay usingchemiluminescence technique on ADVIA Centaur® XP. Serum TSH levels were also analyzed twice daily; in the morning (0800 to 0900 hours) and afternoon (1400 to 1600 hours). Data was analyzed using SPSS version 24. Frequency and percentages were calculated for qualitative variables like gender and pre-analytical variables. Test of significance Mann-Whitney U-test was applied and p-value <0.05 was taken as significant. Results: Mean age of subjects was 23 ± 3.4 years. Change in circadian rhythm was observed in 17 (28%) males and 14 (36%) females. Statistically significant association was found between morning and evening TSH levels, while no change was observed in TSH level by early and late centrifugation of samples. Conclusion: TSH levels vary significantly between blood samples collected at different timings of the day from the same person. TSH is resistant to degradation, immunologically stable, and reasonably insensitive to potential problems associated with routine specimen handling, when measured by immunoassay technique. Therefore, it is helpful in large epidemiological studies and small size laboratory, which require long transportation time and storage.

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Section: S105supporting

confidence: 62%

Effect of Preanalytical Variables on Serum Thyroid Stimulating Hormone Analysis by Chemiluminescence Method

Rehman

Asif

Malik

et al. 2021

PAFMJ

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“…Measurement of fT4 during pregnancy is complicated by pregnancy-related increases in thyroxine binding globulin concentrations, increased total thyroid hormone levels, lower levels of albumin in blood, and circulating human chorionic gonadotropin (hCG). 86–88 The gold-standard method of measuring free thyroid hormones (which uses equilibrium dialysis as the first step, and which was used in one of the reviewed studies 51 ) can most accurately quantify fT4 levels during pregnancy, 80 but is costly. 87,89 All reviewed studies of maternal hypothyroxinemia, and all but one 51 study of maternal fT4, used immunoassays to assess fT4 rather than the gold-standard equilibrium dialysis approach.…”

Section: Discussionmentioning

confidence: 99%

Maternal Thyroid Function During Pregnancy or Neonatal Thyroid Function and Attention Deficit Hyperactivity Disorder

et al. 2019

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Background: Attention Deficit Hyperactivity Disorder (ADHD) is the most common neurobehavioral disorder in children, yet its etiology is poorly understood. Early thyroid hormone disruption may contribute to the development of ADHD. Disrupted maternal thyroid hormone function has been associated with adverse neurodevelopmental outcomes in children. Among newborns, early-treated congenital hypothyroidism has been consistently associated with later cognitive deficits. Methods: We systematically reviewed literature on the association between maternal or neonatal thyroid hormones and ADHD diagnosis or symptoms. We searched Embase, Pubmed, Cinahl, PsycInfo, ERIC, Medline, Scopus, and Web of Science for articles published or available ahead of print as of April 2018. Results: We identified 28 eligible articles: 16 studies of maternal thyroid hormones, seven studies of early-treated congenital hypothyroidism, and five studies of neonatal thyroid hormones. The studies provide moderate evidence for an association between maternal thyroid hormone levels and offspring ADHD, some evidence for an association between early-treated congenital hypothyroidism and ADHD, and little evidence for an association between neonatal thyroid hormone levels and later ADHD. Conclusions: The reviewed articles suggest an association between maternal thyroid function and ADHD, and possibly between early-treated congenital hypothyroidism and ADHD. Study limitations, however, weaken the conclusions in our systematic review, underlining the need for more research. Importantly, there was much variation in measurement of thyroid hormone function and of ADHD symptoms. Recommendations for future research include using population-based designs, attending to measurement issues for thyroid hormones and ADHD, considering biologically relevant covariates (e.g., iodine intake), and assessing non-linear dose–responses.

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“…Intra- and interassay CVs for T3, T4, and TSH were

. We previously established the reliability of MoBa maternal plasma for measurement of thyroid hormone concentrations, considering delays in processing and storage and freeze-thaw cycles ( Villanger et al. 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Prenatal Phthalates, Maternal Thyroid Function, and Risk of Attention-Deficit Hyperactivity Disorder in the Norwegian Mother and Child Cohort

Engel

Villanger

Nethery

et al. 2018

Environ Health Perspect

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Background:There is growing concern that phthalate exposures may have an impact on child neurodevelopment. Prenatal exposure to phthalates has been linked with externalizing behaviors and executive functioning defects suggestive of an attention-deficit hyperactivity disorder (ADHD) phenotype.Objectives:We undertook an investigation into whether prenatal exposure to phthalates was associated with clinically confirmed ADHD in a population-based nested case–control study of the Norwegian Mother and Child Cohort (MoBa) between the years 2003 and 2008.Methods:Phthalate metabolites were measured in maternal urine collected at midpregnancy. Cases of ADHD (n=297) were obtained through linkage between MoBa and the Norwegian National Patient Registry. A random sample of controls (n=553) from the MoBa population was obtained.Results:In multivariable adjusted coexposure models, the sum of di-2-ethylhexyl phthalate metabolites (∑DEHP) was associated with a monotonically increasing risk of ADHD. Children of mothers in the highest quintile of ∑DEHP had almost three times the odds of an ADHD diagnosis as those in the lowest [OR=2.99 (95% CI: 1.47, 5.49)]. When ∑DEHP was modeled as a log-linear (natural log) term, for each log-unit increase in exposure, the odds of ADHD increased by 47% [OR=1.47 (95% CI: 1.09, 1.94)]. We detected no significant modification by sex or mediation by prenatal maternal thyroid function or by preterm delivery.Conclusions:In this population-based case–control study of clinical ADHD, maternal urinary concentrations of DEHP were monotonically associated with increased risk of ADHD. Additional research is needed to evaluate potential mechanisms linking phthalates to ADHD. https://doi.org/10.1289/EHP2358

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Effects of Sample Handling and Analytical Procedures on Thyroid Hormone Concentrations in Pregnant Women’s Plasma

Cited by 12 publications

References 27 publications

Effect of Preanalytical Variables on Serum Thyroid Stimulating Hormone Analysis by Chemiluminescence Method

Effect of Preanalytical Variables on Serum Thyroid Stimulating Hormone Analysis by Chemiluminescence Method

Maternal Thyroid Function During Pregnancy or Neonatal Thyroid Function and Attention Deficit Hyperactivity Disorder

Prenatal Phthalates, Maternal Thyroid Function, and Risk of Attention-Deficit Hyperactivity Disorder in the Norwegian Mother and Child Cohort

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