Effects of Salvia miltorrhiza in neural differentiation of rat mesenchymal stem cells with optimized protocol

Hu, Liang; Yu, Jialin; Li, Fang; Boman, Chen; Li, Lu‐Quan; Liu, Guan-xin

doi:10.1016/j.jep.2011.04.043

Cited by 9 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…No primeiro estudo as células apresentaram rá-pida mudança morfológica, além da expressão de marcadores neurais. Atualmente, além da utilização dos agentes químicos, os protocolos baseiam-se na utilização de fatores de crescimento (Naghdi et al 2009, Bae et al 2011, Nourbakhsh et al 2011, fatores neurotrópicos (Barzilai et al 2008), líquido cefalorraquidiano autólogo (Ye et al 2011) e ϐitoterapia (Hu et al 2011). Particularmente na espécie equina, os estudos estão focados na demonstração da capacidade de diferenciação das CTMs-MO principalmente em tecidos de origem mesodermal, sendo inexistente ou escassa a avaliação do potencial de transdiferenciação neural.…”

Section: Introductionunclassified

Potencial de transdiferenciação neural das células-tronco mesenquimais da medula óssea de equino

et al. 2012

View full text Add to dashboard Cite

The first studies showing the potential of neural transdifferentiation of mesenchymal stem cells (MSCs) from bone marrow (BM) were conducted in camundogos and humans in the early 2000s. After this period, the number of research and publications with the same purpose increased, but with rare or scarce studies in horses. The aim of this study was to evaluate in vitro neuronal transdifferentiation potential of MSCs from equine BM using two protocols: P1 (forksolin and retinoic acid) and P2 (2-βmecarptoetanol). After confirming the mesenchymal lineages, by positivity for the marker CD90 (X=97.94%), negative for the marker CD34 and positive response for osteogenic differentiation, MSCs were subjected to neural transdifferentiation (P1 and P2) for morphological analysis and expression of neural markers GFAP and β3 tubulin by flow cytometry. The results revealed morphological changes in varying degrees between the tested protocols. In protocol 1, twenty four hours after incubation with the media of neural differentiation, a large proportion of cells (>80%) had similar morphology to neural cells, characterized by retraction of cellular body and a large number of cytoplasmic extension (filopodia). However, comparatively, within the first 30 minutes after exposure to the antioxidant β-mercaptoethanol (P2) MSCs showed rapid morphological changes characterized mainly by retraction of cellular body and less cytoplasmic extension. It was also evidenced with the use of this protocol, lower cellular adhesion after exposure to media when compared to P1. Regarding the immunophenotyping analysis it was observed a higher (P<0.001) expression of the markers GFAP and β3 tubulin at the end of P2 compared to P1. The ability of MSCs to generate cell types related to neural lineage is complex and multifactorial, depending not only of inducing agents, but also the environment in which these cells will be cultivated. Thus a greater number of studies are necessary to better understand the process of neural transdifferentiation of MSCs from equine.INDEX TERMS: Horse, neural differentiation, adult stem cells, neural markers.

show abstract

Section: Introductionunclassified

Potencial de transdiferenciação neural das células-tronco mesenquimais da medula óssea de equino

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Hu et al . (2011) suggested that the transplantation of MSCs with SM may be an efficient way in the treatment of neurodegenerative diseases . These results proposed a combination treatment of MSCs with SM could help increase the establishment of transplanted MSCs, leading to the restoration of the infarcted brain region by decreasing apoptosis and enhancing the survival of MSCs.…”

Section: Discussionmentioning

confidence: 99%

“…This study differs from other previous studies which confirmed the individual components at the site of injury. One study suggested that SM induced the differentiation of rat MSCs into neurons with neurophysiological functions . Since MSCs can differentiate into neuron‐like cells in vitro , subsequent studies suggested that the recovery of the infarcted area through MSCs transplantation is related to angiogenesis enhancing glial‐axonal remodelling and functional recovery on the region .…”

Section: Discussionmentioning

confidence: 99%

“…One study suggested that SM induced the differentiation of rat MSCs into neurons with neurophysiological functions. [41] Since MSCs can differentiate into neuron-like cells in vitro, subsequent studies suggested that the recovery of the infarcted area through MSCs transplantation is related to angiogenesis enhancing glial-axonal remodelling and functional recovery on the region. [23,42] However, the degree of differentiation of transplanted MSCs was poor, and the effects of cell transplantation are unlikely to exert a strong beneficial effect for treatment of stroke due to ischemia.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Salvia miltiorrhiza enhances the survival of mesenchymal stem cells under ischemic conditions†

Kim

Lee

et al. 2018

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

ObjectivesTo validate the enhanced therapeutic effect of Salvia miltiorrhiza Bunge (SM) for brain ischemic stroke through the anti‐apoptotic and survival ability of mesenchymal stem cells (MSCs).MethodsThe viability and the expression level of cell apoptotic and survival‐related proteins in MSCs by treatment of SM were assessed in vitro. In addition, the infarcted brain region and the behavioural changes after treatment of MSCs with SM were confirmed in rat middle cerebral artery occlusion (MCAo) models.Key findingsWe demonstrated that SM attenuates apoptosis and improves the cell viability of MSCs. In the rat MCAo model, the recovery of the infarcted region and positive changes of behaviour are observed after treatment of MSCs with SM.ConclusionsThe therapy using SM enhances the therapeutic effect for brain ischemic stroke by promoting the survival of MSCs. This synergetic effect thereby proposes a new experimental approach of traditional Chinese medicine and stem cell‐based therapies for patients suffering from a variety of diseases.

show abstract

“…A standardized fraction of S. miltiorrhiza , PF2401-SF, inhibited iNOS expression and NO production and exhibited anti-inflammatory activity on LPS-activated RAW 264.7 macrophages [ 211 ]. S. miltiorrhiza has also been found to efficiently induce neuron cell differentiation from rat mesenchymal stem cells [ 212 ]. While induced pluripotent stem cells (iPSCs) have the potential to differentiate into neural lineages, S. miltiorrhiza could promote the differentiation potential in vitro, and in vivo enhanced the survival and neural differentiation of transplanted iPSCs-derived neurons [ 213 ].…”

Section: Neuroprotective Natural Products For Admentioning

confidence: 99%

Neuroprotective Natural Products for Alzheimer’s Disease

Chen

Drew

Berney

et al. 2021

Cells

115

View full text Add to dashboard Cite

Alzheimer’s disease (AD) is the number one neurovegetative disease, but its treatment options are relatively few and ineffective. In efforts to discover new strategies for AD therapy, natural products have aroused interest in the research community and in the pharmaceutical industry for their neuroprotective activity, targeting different pathological mechanisms associated with AD. A wide variety of natural products from different origins have been evaluated preclinically and clinically for their neuroprotective mechanisms in preventing and attenuating the multifactorial pathologies of AD. This review mainly focuses on the possible neuroprotective mechanisms from natural products that may be beneficial in AD treatment and the natural product mixtures or extracts from different sources that have demonstrated neuroprotective activity in preclinical and/or clinical studies. It is believed that natural product mixtures or extracts containing multiple bioactive compounds that can work additively or synergistically to exhibit multiple neuroprotective mechanisms might be an effective approach in AD drug discovery.

show abstract

Effects of Salvia miltorrhiza in neural differentiation of rat mesenchymal stem cells with optimized protocol

Cited by 9 publications

References 20 publications

Potencial de transdiferenciação neural das células-tronco mesenquimais da medula óssea de equino

Potencial de transdiferenciação neural das células-tronco mesenquimais da medula óssea de equino

Salvia miltiorrhiza enhances the survival of mesenchymal stem cells under ischemic conditions†

Neuroprotective Natural Products for Alzheimer’s Disease

Contact Info

Product

Resources

About