Effects of salts and the surface hydrophobicity of proteins on partitioning in aqueous two-phase systems containing thermoseparating ethylene oxide-propylene oxide copolymers

Preliminary application of light-pH sensitive recycling aqueous two-phase systems to purification of lipase

“…In recent years, many scientists have made their great efforts on exploring recyclable copolymers. It was reported that ethylene oxide-propylene oxide copolymers (EOPO) could be recycled by temperature [3]. Then random copolymer of EO and PO modified with a hydrophobical aliphatic C 14 H 29 -group (HM-EOPO) was synthesized by Johansson et al [4].…”

Separation of active laccases from Pleurotus sapidus culture supernatant using aqueous two-phase systems in centrifugal partition chromatography

“…The biphasic character of ATPS is achieved by adding phase forming components like two incompatible polymers, one polymer and one salt or two different salts above a critical amount and a certain temperature. Classical ATPS could be proven as gentle and activity preserving extraction systems for biotechnological products [14][15][16]. The combination of using ATPS as phase system in CPC offers new operating windows for e.g., separating fragile or large biochemical compounds which might not be processable by classical solid liquid chromatography (e.g., due to size exclusion limitations due to diffusion or molecular damage by adsorption to the stationary phase's surface).…”

Block copolymer micellization induced microphase mass transfer: Partition of Pd(II), Pt(IV) and Rh(III) in three-liquid-phase systems of S201–EOPO–Na2SO4–H2O

“…Aqueous two-phase extraction (ATPE), cloud point extraction (CPE), reverse micellar extraction (RME) and extraction with thermo-separating polymers are all involved in this category [2]. New phase formation results in the uneven partition of target molecules such as small organic molecules [3,4], biomolecules [5,6], and small metal ion species [7,8].…”