Effects of saliva or serum coating on adherence of Streptococcus oralis strains to titanium

Dorkhan, Marjan; Paz, Luis Enrique Chávez de; Skepö, Marie; Svensäter, Gunnel; Davies, Julia R.

doi:10.1099/mic.0.054536-0

Cited by 37 publications

(34 citation statements)

References 29 publications

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“…In contrast, Bollen et al reported that Ra values less than 0.2 µm do not affect bacterial adhesion (28), and a study by Nascimento et al showed that bacterial adhesion to titanium and zirconium was insensitive to Ra (29). However, Dorkhan et al reported high bacterial adhesion on rough surfaces coated with saliva (30) and found that adhesion to composite resin is not influenced by surface characteristics. Yamamoto et al reported that adhesion to fillers in resin is affected by Ra (31).…”

Section: Discussionmentioning

confidence: 84%

Relevance of surface characteristics in the adhesiveness of polymicrobial biofilms to crown restoration materials

et al. 2018

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We used a polymicrobial (PM) biofilm model to examine associations of bacterial adhesiveness with surface characteristics of various dental materials. Four types of dental materials (apatite pellet, zirconia, ceramic, and composite resin) with rough and mirror surfaces were used. Surface roughness, surface free energy, zeta potential, and colony-forming units (CFUs) of the biofilm formations were measured. Biofilms were cultured for 24 h under anaerobic conditions, plated onto blood agar medium, and anaerobically cultured for 4 days. After culturing, CFU per mm 2 was calculated, and samples were observed under a scanning electron microscope. Means and standard deviations of the experimental data were estimated, and one-way ANOVA and Tukey multiple comparison assays were performed. Pearson correlation coefficients were obtained for the CFU and surface characteristics. Surface roughness and surface free energy appeared to affect generation of PM biofilms on oral materials, and zeta potential was involved in generation of PM biofilms on mirrorground oral materials.

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Section: Discussionmentioning

confidence: 84%

Relevance of surface characteristics in the adhesiveness of polymicrobial biofilms to crown restoration materials

et al. 2018

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“…S. gordonii was identified by positive phenotypic tests for N -acetyl-glucosaminidase, N -acetylgalactosaminidase, α-fucosidase and β-galactosidase while identification of S. mitis was based on positive phenotypic tests for N -acetyl-galactosaminidase, N -acetyl- glucosaminidase, β-galactosidase and sialidase . Identification of S. sanguinis was based on phenotypic tests negative for sialidase, arbinosidase, L-fucosidase, α-glucosidase and firm adherence to MSA agar and S. oralis was identified based on positive phenotypic tests for N -acetylglucosaminidase and sialidase and a negative test for D-fucosidase, in addition to sequencing of the gdh and ddl genes [24]. Bacteria were grown on blood agar in an atmosphere of 5% CO 2 in air at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Adherence of human oral keratinocytes and gingival fibroblasts to nano-structured titanium surfaces

et al. 2014

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BackgroundA key element for long-term success of dental implants is integration of the implant surface with the surrounding host tissues. Modification of titanium implant surfaces can enhance osteoblast activity but their effects on soft-tissue cells are unclear. Adherence of human keratinocytes and gingival fibroblasts to control commercially pure titanium (CpTi) and two surfaces prepared by anodic oxidation was therefore investigated. Since implant abutments are exposed to a bacteria-rich environment in vivo, the effect of oral bacteria on keratinocyte adhesion was also evaluated.MethodsThe surfaces were characterized using scanning electron microscopy (SEM). The number of adhered cells and binding strength, as well as vitality of fibroblasts and keratinocytes were evaluated using confocal scanning laser microscopy after staining with Live/Dead Baclight. To evaluate the effect of bacteria on adherence and vitality, keratinocytes were co-cultured with a four-species streptococcal consortium.ResultsSEM analysis showed the two anodically oxidized surfaces to be nano-structured with differing degrees of pore-density. Over 24 hours, both fibroblasts and keratinocytes adhered well to the nano-structured surfaces, although to a somewhat lesser degree than to CpTi (range 42-89% of the levels on CpTi). The strength of keratinocyte adhesion was greater than that of the fibroblasts but no differences in adhesion strength could be observed between the two nano-structured surfaces and the CpTi. The consortium of commensal streptococci markedly reduced keratinocyte adherence on all the surfaces as well as compromising membrane integrity of the adhered cells.ConclusionBoth the vitality and level of adherence of soft-tissue cells to the nano-structured surfaces was similar to that on CpTi. Co-culture with streptococci reduced the number of keratinocytes on all the surfaces to approximately the same level and caused cell damage, suggesting that commensal bacteria could affect adherence of soft-tissue cells to abutment surfaces in vivo.

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“…However, we observed the contrary in the S. oralis 4087 strain, on which saliva apparently exerts a negative influence on its biofilm-forming capacity. Such strain-specific effects of saliva on S. oralis have recently been described and putatively been associated with the expression of specific adhesins (81).…”

Section: Discussionmentioning

confidence: 99%

Protective Mechanisms of Respiratory Tract Streptococci against Streptococcus pyogenes Biofilm Formation and Epithelial Cell Infection

Fiedler

Riani

Koczan

et al. 2013

Appl Environ Microbiol

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bStreptococcus pyogenes (group A streptococci [GAS]) encounter many streptococcal species of the physiological microbial biome when entering the upper respiratory tract of humans, leading to the question how GAS interact with these bacteria in order to establish themselves at this anatomic site and initiate infection. Here we show that S. oralis and S. salivarius in direct contact assays inhibit growth of GAS in a strain-specific manner and that S. salivarius, most likely via bacteriocin secretion, also exerts this effect in transwell experiments. Utilizing scanning electron microscopy documentation, we identified the tested strains as potent biofilm producers except for GAS M49. In mixed-species biofilms, S. salivarius dominated the GAS strains, while S. oralis acted as initial colonizer, building the bottom layer in mixed biofilms and thereby allowing even GAS M49 to form substantial biofilms on top. With the exception of S. oralis, artificial saliva reduced single-species biofilms and allowed GAS to dominate in mixed biofilms, although the overall two-layer structure was unchanged. When covered by S. oralis and S. salivarius biofilms, epithelial cells were protected from GAS adherence, internalization, and cytotoxic effects. Apparently, these species can have probiotic effects. The use of Affymetrix array technology to assess HEp-2 cell transcription levels revealed modest changes after exposure to S. oralis and S. salivarius biofilms which could explain some of the protective effects against GAS attack. In summary, our study revealed a protection effect of respiratory tract bacteria against an important airway pathogen and allowed a first in vitro insight into local environmental processes after GAS enter the respiratory tract.

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Effects of saliva or serum coating on adherence of Streptococcus oralis strains to titanium

Cited by 37 publications

References 29 publications

Relevance of surface characteristics in the adhesiveness of polymicrobial biofilms to crown restoration materials

Relevance of surface characteristics in the adhesiveness of polymicrobial biofilms to crown restoration materials

Adherence of human oral keratinocytes and gingival fibroblasts to nano-structured titanium surfaces

Protective Mechanisms of Respiratory Tract Streptococci against Streptococcus pyogenes Biofilm Formation and Epithelial Cell Infection

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