Effects of salinity stress on neurotransmission, energy metabolism, and anti-oxidant biomarkers of Carcinus maenas from two estuaries of the NW Iberian Peninsula

Rodrigues, Aurélie Pinto; Oliveira, Patrícia; Guilhermino, Lúcia; Guimarães, Laura

doi:10.1007/s00227-012-1992-8

Cited by 49 publications

(30 citation statements)

References 85 publications

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“…While ROS has long been known to have the potential to cause oxidative damage during exposure to abiotic stressors (reviewed by Lushchak, 2011), including salinity (e.g. Pinto Rodrigues et al, 2012), ROS are far from being solely harmful molecules. A growing number of studies demonstrate that ROSmediated signaling is involved in maintaining redox balance and avoiding ROS-induced damage in a wide diversity of organisms and systems: within the kidney, for example, reports show how NaClinduced ROS formation is involved in the activation of transcription factors upregulating transcription of osmoprotective genes (Zhou et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function?

Rivera‐Ingraham

Barri

Boël

et al. 2015

Journal of Experimental Biology

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Osmoregulating decapods such as the Mediterranean green crab Carcinus aestuarii possess two groups of spatially segregated gills: anterior gills serve mainly respiratory purposes, while posterior gills contain osmoregulatory structures. The co-existence of similar tissues serving different functions allows the study of differential adaptation, in terms of free radical metabolism, upon salinity change. Crabs were immersed for 2 weeks in seawater (SW, 37 ppt), diluted SW (dSW, 10 ppt) and concentrated SW (cSW, 45 ppt). Exposure to dSW was the most challenging condition, elevating respiration rates of whole animals and free radical formation in hemolymph (assessed fluorometrically using C-H 2 DFFDA). Further analyses considered anterior and posterior gills separately, and the results showed that posterior gills are the main tissues fueling osmoregulatory-related processes because their respiration rates in dSW were 3.2-fold higher than those of anterior gills, and this was accompanied by an increase in mitochondrial density (citrate synthase activity) and increased levels of reactive oxygen species (ROS) formation (1.4-fold greater, measured through electron paramagnetic resonance). Paradoxically, these posterior gills showed undisturbed caspase 3/ 7 activity, used here as a marker for apoptosis. This may only be due to the high antioxidant protection that posterior gills benefit from [superoxide dismutase (SOD) in posterior gills was over 6 times higher than in anterior gills]. In conclusion, osmoregulating posterior gills are better adapted to dSW exposure than respiratory anterior gills because they are capable of controlling the deleterious effects of the ROS production resulting from this salinity-induced stress.

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Section: Discussionmentioning

confidence: 99%

Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function?

Rivera‐Ingraham

Barri

Boël

et al. 2015

Journal of Experimental Biology

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“…Biomarkers of oxidative stress have been among the most commonly used biomarkers of cellular stress for habitat quality assessment, namely lipid peroxidation (LPO), catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferase (GST) activities, used mainly as indicators of cellular stress resultant from environmental contamination originated by agricultural, industrial and urban pollutants, such as heavy metals, PCBs and PAHs (Ferreira et al, 2005;Amado et al, 2006). However, environmental variables, strongly related to seasonality and extreme natural events, such as temperature and salinity, are known to have a significant effect on oxidative stress biomarkers (Freire et al, 2011;Rodrigues et al, 2012;Vinagre et al, 2012;Madeira et al, 2013). Hence, these are confounding factors that may result in difficulties in the interpretation of biomarkers response patterns.…”

Section: Introductionmentioning

confidence: 99%

Effect of increasing temperature in the differential activity of oxidative stress biomarkers in various tissues of the Rock goby, Gobius paganellus

Vinagre

Madeira

Mendonça

et al. 2014

Marine Environmental Research

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“…Obviously, energy homeostasis is important for animals to survive stressors (Rodrigues et al, 2012;Giacomin et al, 2014). In the present study, energy status was significantly higher in group S4.…”

Section: Discussionmentioning

confidence: 65%

Effects of periodical salinity fluctuation on the growth, molting, energy homeostasis and molting-related gene expression of Litopenaeus vannamei

Zhang

Guo

Wang

et al. 2016

J. Ocean Univ. China

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To determine the response of Litopenaeus vannamei to periodical salinity fluctuation, a 30-day experiment was conducted in laboratory. In this experiment, two salinity fluctuation amplitudes of 4 (group S4) and 10 (group S10) were designed. The constant salinity of 30 (group S0) was used as the control. Levels of shrimp growth, molting frequency (MF), cellular energy status (ATP, ADP and AMP), as well as the expression of genes encoding molt-inhibiting hormone (MIH), crustacean hyperglycemic hormone (CHH), ecdysteroid-regulated protein (ERP), and energy-related AMP-activated protein kinase (AMPK) were determined. The results showed that periodical salinity fluctuation significantly influenced all indicators except MF which ranged from 13.3% in group S10 to15.4% in group S4. In comparison with shrimps cultured at the constant salinity of 30, those in group S4 showed a significant elevation in growth rate, food conversion efficiency, cellular energy status, ERP and MIH gene transcript abundance, and a significant reduction in CHH and AMPK transcript abundance (P < 0.05). However, salinity fluctuation of 10 only resulted in a significant variation in MIH and CHH gene expression when compared to the control (P < 0.05). According to our findings, L. vannamei may be highly capable of tolerating salinity fluctuation. When ambient salinity fluctuated at approx. 4, the increased MF and energy stores in organisms may aid to promoting shrimp growth.

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Effects of salinity stress on neurotransmission, energy metabolism, and anti-oxidant biomarkers of Carcinus maenas from two estuaries of the NW Iberian Peninsula

Cited by 49 publications

References 85 publications

Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function?

Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function?

Effect of increasing temperature in the differential activity of oxidative stress biomarkers in various tissues of the Rock goby, Gobius paganellus

Effects of periodical salinity fluctuation on the growth, molting, energy homeostasis and molting-related gene expression of Litopenaeus vannamei

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