Effects of salinity stress on proline content and expression ofΔ¹-pyrroline-5-carboxylate synthaseandvacuolar-type H⁺subunit Egenes in wheat

Abstract

To evaluate the responses of some important agronomic traits of 14 bread wheat cultivars, a split plot experiment was conducted based on a randomized complete block design under non-stress, moderate and severe salinity stress conditions. In this study, Backcross and Roshan were identified as the most salinity-tolerant cultivars, while Mihan and Shirudi were considered as the most salinity-sensitive cultivars. The proline content, as well as Δ1-pyrroline-5-carboxylate synthase (P5CS) and vacuolar-type H+-ATPase subunit E (W36) gene expression levels, were examined in these cultivars under normal, moderate and high salinity stress conditions. The proline content and P5CS gene expression level increased with a rise in NaCl concentration. Further, a direct relationship was observed between the proline content and P5CS gene expression in all samples. Our results showed that W36 gene expression in Backcross and Roshan cultivars, as the most resistant ones, strongly increased with elevation of the NaCl concentrations. On the other hand, in the sensitive cultivars such as Mihan and Shirudi, small changes were observed in the gene expression levels with rising salinity levels. Additionally, Backcross and Roshan cultivars had the highest proline content as well as P5CS and W36 genes expression levels under moderate and high salinity stress levels.

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“…Numerous studies have tried to link the increase of soluble sugars to salinity tolerance and the present result agrees with results from wheat genotypes treated with higher salinity level resulted in a significant increase in sugars and proline in some genotypes (Dadkhah and Rassam, 2016;Akbar et al, 2021Akbar et al, , 2022. Simultaneously, proline is also a critical component for osmoprotection in many plants, and one of the most common responses of many plants subjected to abiotic stresses is its accumulation (Goharrizi et al, 2020;Liu et al, 2020;Masarmi et al, 2023). It has been reported that proline plays a protective role in plants exposed to diverse stresses and is thought to be acting as a cellular osmotic regulator.…”

Assessing the air salinity on agro-physiological response of Brassica oleracea var. capitata and Brassica oleracea var. botrytis

“…As a result of this osmotic stress, the plants respond to an increased accumulation of proline (Table 4). Increased salt concentration in the environment causes an increase in plant proline accumulation (Goharrizi et al, 2020). Proline accumulation for the plant will differ depending on its resistance to osmotic stress.…”

Integrated Metabolomics and Transcriptomic Analysis of Hepatopancreas in Different Living Status Macrobrachium nipponense in Response to Hypoxia

“…The knockout of P5CS has indicated that it is necessary for mitochondrial respiratory complex organization [ 61 ]. With the development of research, P5CS in wheat has been found to respond to changes in salinity in the environment [ 62 ]. However, the expression profile of P5CS in wheat is the complete opposite of Mn-P5CS in M. nipponense under hypoxia.…”