Effects of salinities on the gene expression of a (NAD+)-dependent glycerol-3-phosphate dehydrogenase in Dunaliella salina

Cai, Hui; Lao, Yong-Min; Jiang, Jianguo

doi:10.1016/j.scitotenv.2010.12.038

Cited by 49 publications

(47 citation statements)

References 24 publications

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“…** indicates extremely significant difference between them (P<0.01) increasing salt stress (Goyal, 2007a;2007b). The key enzyme, GPD1, has two independent functional domains, glycerol-3-phosphate dehydrogenase (G3PDH) and phosphoserine phosphatase (SerB), which localizes in the chloroplast (Cai et al, 2013) and is highly expressed following hyper-or hypo-salinity stress for two hours, same with other results (Chen et al, 2011). Although the enzymes GPD1, DAK, DHR, and GPPase 1 in the glycerol pathway are regulated by salt stress, GPD1 drives the pathway and controls glycerol synthesis (Chen et al, 2012).…”

Section: Discussionsupporting

confidence: 77%

“…Relative qPCR was performed with the ABI PRISM ® 7900 Real-Time PCR System (Applied Biosystems, CA, USA) using the FastStart Universal SYBR Green Master (ROX; Roche, Basel, Switzerland). The following actin primers were used for endogenous control: 5'-ACCACACCTTCTTCAACGA-3' and 5'-GG ATGGCTACATACATGGCA-3' (Chen et al, 2011). Other selected genes involved in the metabolic processes in D. salina were analyzed using the primers shown in Table S1.…”

Section: Analysis Of Gene Expression Level Under Osmotic Stressmentioning

confidence: 99%

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Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina

Midoun

Miller

2017

J. Zhejiang Univ. Sci. B

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Abstract:The unicellular green alga Dunaliella salina is well adapted to salt stress and contains compounds (including β-carotene and vitamins) with potential commercial value. A large transcriptome database of D. salina during the adjustment, exponential and stationary growth phases was generated using a high throughput sequencing platform. We characterized the metabolic processes in D. salina with a focus on valuable metabolites, with the aim of manipulating D. salina to achieve greater economic value in large-scale production through a bioengineering strategy. Gene expression profiles under salt stress verified using quantitative polymerase chain reaction (qPCR) implied that salt can regulate the expression of key genes. This study generated a substantial fraction of D. salina transcriptional sequences for the entire growth cycle, providing a basis for the discovery of novel genes. This first full-scale transcriptome study of D. salina establishes a foundation for further comparative genomic studies.

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Section: Discussionsupporting

confidence: 77%

Section: Analysis Of Gene Expression Level Under Osmotic Stressmentioning

confidence: 99%

Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina

Midoun

Miller

2017

J. Zhejiang Univ. Sci. B

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“…Bacterial contamination may be limited by increasing the pH or by treatment with detergent and phenol [86,87]. Contamination by protozoa, zooplankton, and other algae may be reduced by using highly selective culture conditions, such as high salinity [88] and high alkalinity [87]. In addition, closed systems are more likely to be run as continuous systems due to their higher efficiency and significantly lower amounts of contamination of undesirable algae and other organisms [86].…”

Section: Cultivationmentioning

confidence: 99%

Microalgal biofuel revisited: An informatics-based analysis of developments to date and future prospects

et al. 2015

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“…The amounts of glycerol in the harvested cell pellet and centrifugation supernatant were both determined using the sodium periodate reagent method [35]: Cell pellets were re-suspended in 1.5 ml water and 0.2 ml chloroform followed by vigorous mixing. The water phase containing glycerol was recovered by centrifugation at 10,000 g for 10 min at room temperature.…”

Section: Glycerol Determinationmentioning

confidence: 99%

Effects of centrifugal stress on cell disruption and glycerol leakage from Dunaliella salina

Milledge

Abubakar

et al. 2015

Microalgae Biotechnology

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Dunaliella salina accumulates large amounts of intracellular glycerol in response to the increases in salt concentration, thus is a potential source for producing fuel grade glycerol as an alternative to biodiesel-derived crude glycerol. D. salina lacks a cell wall; therefore the mode of harvesting Dunaliella cells is critical to avoid cell disruption caused by extreme engineering conditions. This study explored cell disruption and glycerol leakage of D. salina under various centrifugal stresses during cell harvesting. Results show a centrifugal g-force lower than 5000 g caused little cell disruption, while a g-force higher than 9000 g led to ~40% loss of the intact cells and glycerol yields from the recovered algal pellets. Theoretical calculations of the centrifugal stresses that could rupture Dunaliella cells were in agreement with the experimental results, indicating optimisation of centrifugation conditions is important for recovering intact cells of D. salina enriched in glycerol.

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Effects of salinities on the gene expression of a (NAD+)-dependent glycerol-3-phosphate dehydrogenase in Dunaliella salina

Cited by 49 publications

References 24 publications

Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina

Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina

Microalgal biofuel revisited: An informatics-based analysis of developments to date and future prospects

Effects of centrifugal stress on cell disruption and glycerol leakage from Dunaliella salina

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