Distiller's grains are the main by-products produced in the process of brewing and ethanol production. Although they are rich in nutrients, they are highly susceptible to spoilage. Their unscientific utilization not only causes environmental pollution, but also leads to huge waste of resources. Therefore, it is urgent to carry out research on the resource utilization of distiller's grains. In recent years, various studies have been conducted on the resource utilization of distiller's grains both domestically and internationally. According to the utilization methods, it can be mainly divided into four categories: energy production, agricultural products, food, and chemical raw materials, including the production of biogas, animal feed, flour products, and succinic acid, to achieve the resource utilization of distiller's grains. This article reviews the nutritional composition, production and utilization status of distiller's grains, as well as the challenges and future development directions faced by the resource utilization of distiller's grains, in order to provide reference for the treatment and technical research of distiller's grains.