Effects of sacral nerve stimulation with acupuncture on gut transit time and c-kit expression in colon of rats with slow transit constipation

Zhang, Y.G.; Shao, Wanjin; Gu, Yu; Qiu, Jingbo; Lin, Yi-Ting; Li, G.D.

doi:10.4238/gmr.15038362

Cited by 6 publications

(3 citation statements)

References 35 publications

(32 reference statements)

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“…Thirty-six male mice and 36 female mice were randomly allocated to six groups (12 mice per group with 6F + 6M): a normal control (NC) group, a model control (MC) group, a positive control (PC) group, a low-dose GRG (LD) group, a medium-dose GRG (MD) group, and a high-dose GRG (HD) group. The STC mouse model was developed by administering continuous subcutaneous injections of morphine hydrochloride (2.5 mg/kg/d) for 45 days, as previously described [ 16 ]. After model establishment, the LD, MD, and HD groups were treated by gavage with 4.72 g/kg/d, 9.44 g/kg/d, and 18.88 g/kg/d GRG, respectively, comparable to the clinical doses applied in adults [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Curative Effect and Mechanism of Guiren Runchang Granules on Morphine-Induced Slow Transit Constipation in Mice

Sun

Yan

Jin

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Recent studies have identified the curative effects of traditional Chinese medicine for constipation. The mechanism of action of Guiren Runchang granules (GRGs) in the treatment of slow transit constipation (STC) was evaluated in this study. Here, we assessed the efficacy of GRG by comparing the differences in fecal characteristics, stool weight, and intestinal transit rate (ITR) among 6 groups (n = 12/group), which were administered three concentrations of GRG, mosapride, and saline. The influence of GRG on the SCF/c-kit pathway, AQP4, and serum motilin of mice was assessed through ELISA, western blot, and immunohistochemical analysis. The dry weight of mouse feces at 24 hr and ITR in the MD (medium-dose GRG; 9.44 g/kg/d) and HD (high-dose GRG; 18.88 g/kg/d) groups was higher than that in the MC (model control) group. The serum motilin of morphine-induced mice level was lower in the MC group than in the NC (normal control) group, and this condition was improved in the HD group. The HD group expressed significantly higher levels of SCF and c-kit protein but lower levels of AQP4 and simultaneously presented more SCF-positive and c-kit-positive cells. However, no differences in the serum SCF level were found among the six groups. Certain concentrations of GRG are effective in STC mice, the potential mechanism of which may be associated with repairing the SCF/c-kit pathway and reducing the expression of AQP4 in the colon. GRG improved the serum motilin level but had no influence on the serum SCF level.

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Section: Methodsmentioning

confidence: 99%

Curative Effect and Mechanism of Guiren Runchang Granules on Morphine-Induced Slow Transit Constipation in Mice

Sun

Yan

Jin

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…There is increasing evidence to indicate that it has potential for use in the treatment of constipation. It is reported that polysaccharides of Anemarrhena asphodeloides treat constipation via enhancing gastrointestinal motility [10]. However, the precise effects on the water-eluting component of Anemarrhena asphodeloides Bge.…”

Section: Introductionmentioning

confidence: 99%

The water-eluting component of Anemarrhena asphodeloides Bge. laxative effects on loperamide-induced constipation rats

Li¹,

Liu²,

Sun³

et al. 2020

Preprint

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Background Slow transit functional constipation (STC) is the most common type of chronic constipation,with seriously affecting people's survival and quality of life in the recent years. Based on the safety and small side effects of traditional Chinese medicine, it is urgent to research and develop new medicine for the treatment of constipation. As a traditional Chinese medicine, Anemarrhena asphodeloides Bge. has been reported treat constipation in a long time, and carbohydrate is its active ingredient. Methods ELISA used for detection of serum gastrointestinal hormones and neurotransmitters levels. HE stain was analyzed to evaluate the colon injury. Western blot and RT-PCR detected interstitial cells of Cajal markers (SCF/c-Kit) and aquaporins (AQPs). Results The mainly compositions of oligosaccharide and amino acid from AABW was determined by HPLC. Based on the laxative activities of AABW in the study, AABW obviously enhanced the contents of VIP, Gas, MTL, SP and 5-HT and decreased the levels of NO from loperamide-induced rats by Elisa kits. Additionally, AABW could up-regulate the expressions of SCF, c-Kit, AQP3, VIP and down-regulate AQP8 to repair the damage of colon. Conclusion These findings suggested that AABW, which could treat constipation through mediating intestinal motility and water metabolism, were laxative active constituents from Anemarrhena asphodeloides Bge..

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“…Rats have a mild disposition, good tolerance, and high success rate in experimental research, therefore they are the first choice for the study of STC ( 7 ). The STC rat model was established by continuously feeding diphenoxylate, which can be applied in clinical practice and has many advantages, such as being a simple, economical, and reproducible method ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of bisacodyl on rats with slow transit constipation

Wang

Ling

Zhang

et al. 2018

Braz J Med Biol Res

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The effect of bisacodyl on the treatment of rats with slow transit constipation (STC) was studied. Forty-five female Wister rats were divided into control group, STC group, and STC bisacodyl group. The immunohistochemical method was used to determine interstitial cells of Cajal (ICC) and the expression of c-Kit protein. Body mass and the number of defecations were significantly decreased in the STC group compared with the control group on the 100th day after diphenoxylate administration, while dry weight of feces was significantly increased and the intestinal transit time was prolonged. There were significant differences in the number of defecations, dry weight of feces, and intestinal transit time among the three groups. The number of defecations was higher, dry weight of feces was lower, and intestinal transit time was shorter in the STC bisacodyl group compared to the STC group. In addition, ICC basement membrane dissolution occurred in the colon wall of the STC group. The connection between ICC and surrounding cells was destroyed, and the nucleus shrunken to different degrees. Moreover, c-Kit expression in the STC group was significantly lower than the control group. The connection between ICC and surrounding cells in the STC bisacodyl group was significantly stronger than the STC group, and the number of ICC and the expression of c-Kit were increased. Bisacodyl could reduce the severity of STC in rats by increasing the number of ICC and the expression of c-Kit.

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Effects of sacral nerve stimulation with acupuncture on gut transit time and c-kit expression in colon of rats with slow transit constipation

Cited by 6 publications

References 35 publications

Curative Effect and Mechanism of Guiren Runchang Granules on Morphine-Induced Slow Transit Constipation in Mice

Curative Effect and Mechanism of Guiren Runchang Granules on Morphine-Induced Slow Transit Constipation in Mice

The water-eluting component of Anemarrhena asphodeloides Bge. laxative effects on loperamide-induced constipation rats

Effect of bisacodyl on rats with slow transit constipation

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