Effects of Saccharomyces cerevisiae quorum sensing signal molecules on ethanol production in bioethanol fermentation process

Tian, Jie; Lin, Yuping; Su, Xiaoying; Tan, Honghao; Gan, Chaoyi; Ragauskas, Arthur J.

doi:10.1016/j.micres.2023.127367

Cited by 7 publications

(6 citation statements)

References 40 publications

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“…The study points out that the physiological concentrations reported by Winters et al (2019) do not reflect the natural occurrence observed in a diffusion-limited fermentation environment using industrially relevant strains. Tian et al (2023) studied the effect of the addition of QSMs on the fermentation of S. cerevisiae, and showed that the exogenous addition of Phe-OH promoted the accumulation of ethanol, which was similar to the results of Huang and Reardon (2021a) and provided basic data for using QSMs more than antibiotics in the prevention of contamination during the industrialized bioethanol production. QS has been observed in many microbial species, regulating the many diverse processes, including secretion of virulence factors, bioluminescence, cell adhesion and elongation, population density control, motility, biofilm formation, cell morphology and dimorphism, sporulation and antibiotic production (Miller and Bassler, 2001;Albuquerque and Casadevall, 2012;Wongsuk et al, 2016;Schuster et al, 2017;Padder et al, 2018;Gomez-Gil et al, 2019).…”

Section: Milestones and Hotspots Of Qs Research 21 Milestones Of Qs R...supporting

confidence: 56%

“…Phe-OH has a positive effect on biofilm formation of S. cerevisiae (Zhang et al, 2021b). As a barrier, biofilm can provide a stable internal environment for yeast cells to cope with the adverse environment such as ethanol stress and nutrient insufficiency (Tian et al, 2023). In the production of fuel ethanol, the conversion rate of repeated batch fermentation of S. cerevisiae in biofilm reactor is higher than that of free fermentation.…”

Section: Hotspots Of Qs Researchmentioning

confidence: 99%

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Quorum sensing: cell-to-cell communication in Saccharomyces cerevisiae

Li,

Pan,

Zhang

et al. 2023

Front. Microbiol.

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Quorum sensing (QS) is one of the most well-studied cell-to-cell communication mechanisms in microorganisms. This intercellular communication process in Saccharomyces cerevisiae began to attract more and more attention for researchers since 2006, and phenylethanol, tryptophol, and tyrosol have been proven to be the main quorum sensing molecules (QSMs) of S. cerevisiae. In this paper, the research history and hotspots of QS in S. cerevisiae are reviewed, in particular, the QS system of S. cerevisiae is introduced from the aspects of regulation mechanism of QSMs synthesis, influencing factors of QSMs production, and response mechanism of QSMs. Finally, the employment of QS in adaptation to stress, fermentation products increasing, and food preservation in S. cerevisiae was reviewed. This review will be useful for investigating the microbial interactions of S. cerevisiae, will be helpful for the fermentation process in which yeast participates, and will provide an important reference for future research on S. cerevisiae QS.

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Section: Milestones and Hotspots Of Qs Research 21 Milestones Of Qs R...supporting

confidence: 56%

Section: Hotspots Of Qs Researchmentioning

confidence: 99%

Quorum sensing: cell-to-cell communication in Saccharomyces cerevisiae

Li,

Pan,

Zhang

et al. 2023

Front. Microbiol.

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“…One recent report by Kapetanakis et al had investigated the possibility of engineering a S. cerevisiae strain with knockout mutations of amino acid transporter (Qdr) to limit cross-feeding and propagation of L. fermentum [ 37 ]. Other research avoided stuck fermentation caused by Lactiplantibacillus plantarum using S. cerevisiae quorum sensing signal molecule, 2-phenylethanol [ 38 ]. However, these methods do not address the source of contamination inside the fermentation tank.…”

Section: Discussionmentioning

confidence: 99%

Novel endolysin LysMP for control of Limosilactobacillus fermentum contamination in small-scale corn mash fermentation

Patel,

Lu,

Liu

et al. 2023

Biotechnol Biofuels

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Background Traditional bioethanol fermentation industries are not operated under strict sterile conditions and are prone to microbial contamination. Lactic acid bacteria (LAB) are often pervasive in fermentation tanks, competing for nutrients and producing inhibitory acids that have a negative impact on ethanol-producing yeast, resulting in decreased yields and stuck fermentations. Antibiotics are frequently used to combat contamination, but antibiotic stewardship has resulted in a shift to alternative antimicrobials. Results We demonstrate that endolysin LysMP, a bacteriophage-encoded peptidoglycan hydrolase, is an effective method for controlling growth of LAB. The LysMP gene was synthesized based on the prophage sequence in the genome of Limosilactobacillus fermentum KGL7. Analysis of the recombinant enzyme expressed in E. coli and purified by immobilized metal chelate affinity chromatography (IMAC) showed an optimal lysis activity against various LAB species at pH 6, with stability from pH 4 to 8 and from 20 to 40 °C up to 48 h. Moreover, it retains more than 80% of its activity at 10% ethanol (v/v) for up to 48 h. When LysMP was added at 250 µg/mL to yeast corn mash fermentations containing L. fermentum, it reduced bacterial load by at least 4-log fold compared to the untreated controls and prevented stuck fermentation. In comparison, untreated controls with contamination increased from an initial bacterial load of 1.50 × 107 CFU/mL to 2.25 × 109 CFU/mL and 1.89 × 109 CFU/mL after 24 h and 48 h, respectively. Glucose in the treated samples was fully utilized, while untreated controls with contamination had more than 4% (w/v) remaining at 48 h. Furthermore, there was at least a fivefold reduction in lactic acid (0.085 M untreated contamination controls compared to 0.016 M treated), and a fourfold reduction in acetic acid (0.027 M untreated contamination controls vs. 0.007 M treated), when LysMP was used to treat contaminated corn mash fermentations. Most importantly, final ethanol yields increased from 6.3% (w/v) in untreated contamination samples to 9.3% (w/v) in treated contamination samples, an approximate 50% increase to levels comparable to uncontaminated controls 9.3% (w/v). Conclusion LysMP could be a good alternative to replace antibiotics for mitigation of LAB contamination in biofuel refineries.

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“…The secretion of phenylethyl alcohol and tryptophol is observed in the yeast Saccharomyces cerevisiae during nitrogen starvation, and these compounds regulate cell density and their morphological changes. The possibility of regulating the morphological and biochemical characteristics of different yeast populations using QSMs is currently being investigated in various biotechnological processes ( Table 3 ) [ 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 ].…”

Section: Biocatalysts For Biotechnological Processes Based On Highly ...mentioning

confidence: 99%

“…Another reason for the increase in ethanol yield (carbon substrate conversion efficiency) is a decrease in biomass growth during fermentation so that more substrate can be converted into the desired product. According to studies [ 60 , 61 , 62 , 64 ], QSMs allow the regulation of the yield of ethanol. Interestingly, the addition of phenylethanol in flow bioreactor experiments [ 62 ] reduces the rate of ethanol production and glucose consumption, indicating an effect of this compound on the rate of yeast catabolism.…”

Section: Biocatalysts For Biotechnological Processes Based On Highly ...mentioning

confidence: 99%

Quorum Sensing as a Trigger That Improves Characteristics of Microbial Biocatalysts

et al. 2023

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Quorum sensing (QS) of various microorganisms (bacteria, fungi, microalgae) today attracts the attention of researchers mainly from the point of view of clarifying the biochemical basics of this general biological phenomenon, establishing chemical compounds that regulate it, and studying the mechanisms of its realization. Such information is primarily aimed at its use in solving environmental problems and the development of effective antimicrobial agents. This review is oriented on other aspects of the application of such knowledge; in particular, it discusses the role of QS in the elaboration of various prospective biocatalytic systems for different biotechnological processes carried out under aerobic and anaerobic conditions (synthesis of enzymes, polysaccharides, organic acids, etc.). Particular attention is paid to the biotechnological aspects of QS application and the use of biocatalysts, which have a heterogeneous microbial composition. The priorities of how to trigger a quorum response in immobilized cells to maintain their long-term productive and stable metabolic functioning are also discussed. There are several approaches that can be realized: increase in cell concentration, introduction of inductors for synthesis of QS-molecules, addition of QS-molecules, and provoking competition between the participants of heterogeneous biocatalysts, etc.).

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Effects of Saccharomyces cerevisiae quorum sensing signal molecules on ethanol production in bioethanol fermentation process

Cited by 7 publications

References 40 publications

Quorum sensing: cell-to-cell communication in Saccharomyces cerevisiae

Quorum sensing: cell-to-cell communication in Saccharomyces cerevisiae

Novel endolysin LysMP for control of Limosilactobacillus fermentum contamination in small-scale corn mash fermentation

Quorum Sensing as a Trigger That Improves Characteristics of Microbial Biocatalysts

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