Effects of rutin on the physicochemical properties of skin fibroblasts membrane disruption following UV radiation

Dobrzyńska, Izabela; Gęgotek, Agnieszka; Gajko, Ewelina; Figaszewski, Zbigniew A.

doi:10.1016/j.cbi.2018.01.012

Cited by 19 publications

(8 citation statements)

References 32 publications

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“…RUT has various pharmacological properties, 34 but its potential role in nephroprotection is yet to be proven. Alhoshani et al 35 demonstrated that the antioxidant and anti-inflammatory effects of RUT may prevent CP-induced nephrotoxicity in rats by reducing oxidative stress, inhibiting the interconnected ROS/c-Jun N-terminal kinase (JNK)/Tumor Necrosis Factor (TNF)/P38 Mitogen-activated protein kinase (MAPK) signaling pathways, and repairing the histopathological changes against CP administration.…”

Section: Discussionmentioning

confidence: 99%

Effect of rutin on cisplatin-induced damage in human mesangial cells via apoptotic pathway

Zhang

Wang

et al. 2018

Hum Exp Toxicol

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Cisplatin (CP) is one of the most effective and widely used compounds in the treatment of disease, including cancer, but is known to induce toxicity in patients. Rutin (RUT) is a flavonoid glycoside from Sophora japonica L. that has been shown to possess antioxidative, anti-inflammatory, and antiviral properties. RUT is also known to attenuate cardiotoxicity, isoproterenol-induced cardiac fibrosis, and ischemia/reperfusion-associated hemodynamic alteration, and prevents high glucose-induced renal glomerular endothelial hyperpermeability. In this study, we investigated the effect of RUT on CP-induced nephrotoxicity. CP was used to induce toxicity in human mesangial cells (HMCs), HMCs were pretreated with different concentrations of RUT before being exposed to 10 μg/mL of CP. A positive group was pretreated with antioxidant agent N-acetylcysteine prior to CP administration. At doses between 12.5 and 25 μM, RUT prevented CP-induced reduction in cell viability. Treatment with RUT suppressed intracellular reactive oxygen species and malonic dialdehyde levels and inhibited cell apoptosis. RUT reversed the CP-induced upregulation of p53, cleaved-caspase-3, and increased pro-caspase-3 and pro-caspase-9 levels. In conclusion, the RUT can relieve CP-induced nephrotoxicity by inhibiting the p53/caspase signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Effect of rutin on cisplatin-induced damage in human mesangial cells via apoptotic pathway

Zhang

Wang

et al. 2018

Hum Exp Toxicol

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“…where R is the gas constant, T is temperature, F is the Faraday number, I is the ionic strength of 0.9% NaCl, ε•ε o is the permeability of the electric medium 79 .…”

Section: Assessment Of Surface Charge Density and Zp Of Erythrocytes mentioning

confidence: 99%

Inhibition of interaction between Staphylococcus aureus α-hemolysin and erythrocytes membrane by hydrolysable tannins: structure-related activity study

Olchowik-Grabarek

Sękowski

Bitiucki

et al. 2020

Sci Rep

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The objective of the study was a comparative analysis of the antihemolytic activity against two Staphylococcus aureus strains (8325-4 and NCTC 5655) as well as α-hemolysin and of the membrane modifying action of four hydrolysable tannins with different molecular mass and flexibility: 3,6-bis-O-di-O-galloyl-1,2,4-triO -galloyl-β-d-glucose (T1), 1,2,3,4,5-penta-O-galloyl-β-d-glucose (T2), 3-O-galloyl-1,2-valoneoyl-β-d-glucose (T3) and 1,2-di-O-galloyl-4,6-valoneoyl-β-d-glucose (T4). We showed that all the compounds studied manifested antihemolytic effects in the range of 5-50 µM concentrations. However, the degree of the reduction of hemolysis by the investigated tannins was not uniform. A valoneoyl group-containing compounds (T3 and T4) were less active. Inhibition of the hemolysis induced by α-hemolysin was also noticed on preincubated with the tannins and subsequently washed erythrocytes. In this case the efficiency again depended on the tannin structure and could be represented by the following order: T1 > T2 > T4 > T3. We also found a relationship between the degree of antihemolytic activity of the tannins studied and their capacity to increase the ordering parameter of the erythrocyte membrane outer layer and to change zeta potential. Overall, our study showed a potential of the T1 and T2 tannins as anti-virulence agents. The results of this study using tannins with different combinations of molecular mass and flexibility shed additional light on the role of tannin structure in activity manifestation. Over the past few years, a significant increase in bacterial resistance to antibiotics and transference of resistance genes from animal to human strains has become a global medical problem. Therefore, constant search for new antimicrobial agents among them being compounds of plant origin, including polyphenols, is ongoing 1,2. In addition to the antibiotic approaches of combating bacteria, anti-virulence strategies have also been considered recently. An anti-virulent strategy assumes a direct effect of compounds on virulent factors by reducing

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“…Moreover, its antioxidant action is also based on the activation of cytoprotective transcription factor Nrf2 [19]. It has been shown that rutin prevents UV-induced changes in skin cell membrane function and, by inhibiting the activity of cyclooxygenases and lipoxygenases as well as non-enzymatic lipid mediators generation, inhibits pro-inflammatory signaling [20,21]. Moreover, rutin exerts cytoprotective effects on cells exposed to various types of physical factors by substantially increasing their viability [22,23].…”

Section: Introductionmentioning

confidence: 99%

Cytoprotective Effect of Ascorbic Acid and Rutin against Oxidative Changes in the Proteome of Skin Fibroblasts Cultured in a Three-Dimensional System

Gęgotek

Jarocka-Karpowicz

2020

Nutrients

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The combination of ascorbic acid and rutin, commonly used in oral preparations for their antioxidant and anti-inflammatory properties, can also be used to protect skin cells from the effects of UV radiation in sunlight. Here, we tested the potential protective effect of ascorbic acid and rutin used together in UVB-irradiated human skin fibroblasts, and assessed the proteomic profile of these cells, grown in a three-dimensional (3D) system. Proteomic findings revealed a combined effect of ascorbic acid and rutin in UV-irradiated fibroblasts against overexpression of pro-inflammatory signaling proteins and DNA reorganization/expression. These effects were not observed when cells were treated with either compounds alone. The antioxidant effects of ascorbic acid and rutin also prevented protein modifications by lipid peroxidation products. Further, ascorbic acid stimulated rutin-protein adduct formation, which supports intra/extracellular signaling and the Nrf2/ARE antioxidant pathway, contributing to the protective effects against UV-induced oxidative stress. The combined effect of ascorbic acid and rutin suggests that this combination of compounds is potentially effective against skin damage caused by UV radiation.

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Effects of rutin on the physicochemical properties of skin fibroblasts membrane disruption following UV radiation

Cited by 19 publications

References 32 publications

Effect of rutin on cisplatin-induced damage in human mesangial cells via apoptotic pathway

Effect of rutin on cisplatin-induced damage in human mesangial cells via apoptotic pathway

Inhibition of interaction between Staphylococcus aureus α-hemolysin and erythrocytes membrane by hydrolysable tannins: structure-related activity study

Cytoprotective Effect of Ascorbic Acid and Rutin against Oxidative Changes in the Proteome of Skin Fibroblasts Cultured in a Three-Dimensional System

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