Effects of Rubiadin isolated from Prismatomeris connata on anti‐hepatitis B virus activity in vitro

Abstract

Prismatomeris connata was a kind of Rubiaceae plant for treatment of hepatitis, hepatic fibrosis and silicosis. Whereas, the effective components of Prismatomeris connata remains unexplored. The aim of this study was to investigate the inhibitory effects and mechanisms of Rubiadin isolated from Prismatomeris connata against HBV using HepG2.2.15 cells. The levels of hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), and hepatitis B core antigen (HBcAg) in the supernatants or cytoplasm were examined using by enzyme-linked immunosorbent assay. HBV DNA was qualified q-PCR. Rubiadin was isolated by silica gel column. The structure of the compound was elucidated by HPLC, FT-IR, H-NMR, C-NMR and identified as 1,3-Dihydroxy-2-methyl-9, 10-anthraquinone. Rubiadin significantly decreased HBeAg,HBcAg secretion level and inhibit HBV DNA replication. Rubiadin inhibits the proliferation of the cells and HBx protein expression in a dose-dependent manner. The intracellular calcium concentration was significantly reduced. These results demonstrated that Rubiadin could inhibit HepG2.2.15 cells proliferation, reduce the level of HBx expression, and intracellular free calcium, which might become a novel anti-HBV drug candidate.

Anti‐hepatitis B virus activity and hepatoprotective effect of des(rhamnosyl) verbascoside from Lindernia ruellioides in vitro

“…After washing, the membranes were incubated with horseradish peroxidase‐labelled goat anti‐rabbit IgG for 1.5 hr. Then, the bands were illuminated with an enhanced chemiluminescence kit and observed (Peng et al, 2017).…”

Anti-Hepatitis B Virus Activity of Esculetin from Microsorium fortunei In Vitro and In Vivo

“…The HBx protein was evaluated by using an improved method of reference [44]. HepG2.2.15 cells were lysed with a radio-immunoprecipitation assay (RIPA) buffer buffer after treatment with esculetin (12.5, 25, 50, 80 mg/L) and 3TC (100 mg/L) for 72 h, and the protein was quantified with a BCA kit.…”

Chemistry, Biosynthesis, Physicochemical and Biological Properties of Rubiadin: A Promising Natural Anthraquinone for New Drug Discovery and Development

“…A very detailed structural characterization of Rubiadin is given below based on the values obtained from spectroscopic methods including ultraviolet (UV), Fourier-transformed infrared (FTIR), 1 H-nuclear magnetic resonance (NMR), 13 C-NMR and EI-MS. 30 The UV spectra of Rubiadin indicates a λmax at 408 nm for n-π* transition and at 279 and 251 for π-π* transitions. The IR spectra shows absorption bands at 3396 (OH), 2923, (C-H), 1661 (C=O non-chelated) and at 1623, 1589 (C=C< aromatic) representing the characteristic groups of Rubiadin.…”

“…The remaining signals at δ 134.54 (C-8a), 134.44 (C-10a), 132.98 (C-4a), 132.87 (C-6), 131.70 (C-7), 126.70 (C-5) and 126.37 (C-8) are characteristic signals off the unsubstituted aromatic ring of the AQ system. 30 …”