Effects of Rosa damascena on reproductive improvement, metabolic parameters, liver function and insulin-like growth factor-1 gene expression in estradiol valerate induced polycystic ovarian syndrome in Wistar rats

Farhadi-Azar, Mahbanoo; Ghahremani, Mehrdad; Mahboobifard, Fatemeh; Noroozzadeh, Mahsa; Yaghmaei, Parichehreh; Tehrani, Fahimeh Ramezani

doi:10.1016/j.bj.2022.05.003

Cited by 5 publications

(4 citation statements)

References 45 publications

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“…In the present study, the ameliorative effects of Chn/Al-AH in EV-induced POCS rats were investigated for the rst time. To induce the PCOS model in rats, Estrogen and drugs with estrogenic effects are commonly used, as these compounds prompt morphological effects similar to those seen in PCOS [25]. Similar to the ndings of Farhadi-Azar et al [26], Our study indicated that EV is e cient enough to induce PCOS in rats.…”

Section: Discussionsupporting

confidence: 87%

Chitosan/Alginate-loaded Astragalus hamosus shows ameliorating effects on lipid profile, inflammatory and hormonal parameters, and reduces miRNA-222 expression in Polycystic Ovary Syndrome Rats

Bostani,

Kohnehshahri,

Tabatabaei

et al. 2024

Preprint

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Objective This study aimed to investigate the effects of Chitosan/Alginate-loaded Astragalus hamosus extract (AH) on the expression of miRNA-222 and ESR1 genes, pro-inflammatory cytokines, lipid profiles, and sex hormone levels in rats with PCOS-induced by estradiol valerate (EV). Methods 25 female Wistar rats, with an average weight of 180 g, were divided into control and PCOS groups. The PCOS model was induced by a single intramuscular injection of EV (4 mg/kg). After 28 days of PCOS induction, the rats were orally administered Chitosan/Alginate-loaded AH at doses of 5, 10, and 15 mg/kg. Following four weeks of treatment, histological and biochemical parameters, pro-inflammatory cytokines, body weight, and the expression of miRNA-222 and ESR1 genes were evaluated. Results EV-induced PCOS rats exhibited a significant increase in body weight, abnormal lipid profiles, elevated pro-inflammatory cytokine levels, altered sex hormone levels, upregulation of miRNA-222 expression, and downregulation of ESR1 gene expression. The administration of Chitosan/Alginate-loaded AH (Chn/Al-AH) demonstrated ameliorative effects on the PCOS rats, restoring both endocrine and metabolic abnormalities to normal levels and attenuating complications in EV-induced PCOS rats. Notably, it significantly reduced miRNA-222 expression and promoted follicular development at various stages while reducing cystic follicles and increasing corpus luteum formation. Conclusions Chn/Al-AH was found to have beneficial effects in attenuating and improving certain complications in the PCOS rat models.

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Section: Discussionsupporting

confidence: 87%

Chitosan/Alginate-loaded Astragalus hamosus shows ameliorating effects on lipid profile, inflammatory and hormonal parameters, and reduces miRNA-222 expression in Polycystic Ovary Syndrome Rats

Bostani,

Kohnehshahri,

Tabatabaei

et al. 2024

Preprint

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“…set out to evaluate the effects of Rosa damascena (RD) in PCOS rats. The study in 48 animals either treated with metformin or RD extract showed that RD has a potential therapeutic application for alleviating PCOS complications [ 20 ].…”

Section: Also In This Issuementioning

confidence: 99%

“How sad it is! I shall grow old, and horrible, and dreadful” – The ups and downs of cell senescence

Kattner¹

2023

Biomedical Journal

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“…(2) PCOS group (by a single intramuscular injection of estradiol valerate-induced (EV) at a dose of 4 mg/kg/day (dissolved in 0.4 mL of sesame oil) for 28 days); (3) PCOS (4 mg/kg EV BW) + chitosan-propolis nanoparticles (500 mg/kg; 2 times per weeks for 42 consecutive days, gavages); (4) PCOS (4 mg/kg EV) + metformin (150 mg/kg, dissolved in normal saline (NS), daily for 42 consecutive days, gavages). Metformin (45) and EV (46) doses were determined according to previous studies. Duration of metformin and chitosan-propolis nanoparticles treatment were set according to vaginal smear alteration in the estrous cycle and its normality in the metformin group.…”

Section: Groupsmentioning

confidence: 99%

Ameliorative Effect of Chitosan-Propolis Nanoparticles on the Estradiol Valerate-Induced Polycystic Ovary Syndrome Model

Hosseini,

Khodaei,

Hasansagha

et al. 2023

Jundishapur J Nat Pharm Prod

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Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder affecting women. Previous research has shown that PCOS is associated with insulin resistance, oxidative stress, and immune system malfunctions. Also, the antioxidant effects of propolis and the positive effects of chitosan nanoparticles on the reproductive system have been demonstrated in some reports. Objectives: The current study is designed to investigate the protective effects of chitosan-propolis nanoparticle against estradiol valerate-induced (EV) PCOS model of rats compared to metformin (Met) (as a control treatment). Methods: Intramuscular injection of EV (4 mg/kg, 28 days) was used to induce PCOS in rats, followed by oral administration of 500 mg/kg chitosan-propolis nanoparticle for 42 days. Rats were divided into 4 groups: Control, PCOS, metformin (PCOS and 150 mg/kg metformin), and chitosan-propolis nanoparticles (PCOS and chitosan-propolis nanoparticle administration, 500 mg/kg) groups. Results: All animals were subjected to serum factors analysis and histopathological study of ovaries. Estradiol valerate-induced induced PCOS while administration of chitosan-propolis nanoparticle recovered it. The body weight (P < 0.01) and ovarian morphology improved. The serum biochemical parameters, including estrogen (P < 0.05), progesterone (P < 0.001), vitamin D (P < 0.01), calcium (P < 0.01), and insulin resistance index (P < 0.05) were reversed after chitosan-propolis nanoparticle intervention. These EV-induced alterations included inhibited superoxide dismutase (SOD) activity (P < 0.05) and increased malondialdehyde (MDA) level (P < 0.001), and it was demonstrated that chitosan-propolis nanoparticle/Met administered for 42 consecutive days and gavages with EV reversed the oxidative stress factors. Additionally, in EV-treated animals, there was a significant upregulation of certain relative mRNA expressions, such as monocyte chemoattractant protein (MCP) (P < 0.01), interleukin 18 (IL-18) (P < 0.05), and C-reactive protein (CRP) (P < 0.01) genes. These data clearly show that chitosan-propolis nanoparticle/Met may have a protective effect on this inflammatory disorder. Conclusions: Taken together, the final results of this study are consistent with the assumption that chitosan-propolis nanoparticle /Met had ameliorative and protective effects against the harmful effects of EV. Although it is hypothesized that ameliorative effects might have been involved, the fundamental pathways remain to be illuminated.

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Effects of Rosa damascena on reproductive improvement, metabolic parameters, liver function and insulin-like growth factor-1 gene expression in estradiol valerate induced polycystic ovarian syndrome in Wistar rats

Cited by 5 publications

References 45 publications

Chitosan/Alginate-loaded Astragalus hamosus shows ameliorating effects on lipid profile, inflammatory and hormonal parameters, and reduces miRNA-222 expression in Polycystic Ovary Syndrome Rats

Chitosan/Alginate-loaded Astragalus hamosus shows ameliorating effects on lipid profile, inflammatory and hormonal parameters, and reduces miRNA-222 expression in Polycystic Ovary Syndrome Rats

“How sad it is! I shall grow old, and horrible, and dreadful” – The ups and downs of cell senescence

Ameliorative Effect of Chitosan-Propolis Nanoparticles on the Estradiol Valerate-Induced Polycystic Ovary Syndrome Model

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