Effects of ropivacaine, bupivacaine and sufentanil in colon and pancreatic cancer cells in vitro

Bundscherer, Anika; Malsy, Manuela; Gebhardt, Kristina; Metterlein, Thomas; Plank, Christoph; Wiese, C.H.R.; Gruber, Michael; Graf, Bernhard M.

doi:10.1016/j.phrs.2015.03.017

Cited by 50 publications

(48 citation statements)

References 33 publications

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“…Observed differences might be caused by several reasons, but mostly, as we proposed, by application of trypsinization to detach cells from the flask surface and centrifugation. Different ways of cells detachment can lead to different levels of membrane damage and therefore can influence the percent of necrotic and apoptotic cells in population [4]. Bundscherer et al concluded that trypsinization is a gentle method for cell detachment, but can be inadequate for example for metabolomic studies [4].…”

Section: Influence Of Proposed Methods On Cell Death Type Determinatimentioning

confidence: 99%

“…Different ways of cells detachment can lead to different levels of membrane damage and therefore can influence the percent of necrotic and apoptotic cells in population [4]. Bundscherer et al concluded that trypsinization is a gentle method for cell detachment, but can be inadequate for example for metabolomic studies [4]. During centrifugation, some fraction of cells could be lost by removal of unpelleted ones in the supernatant [8].…”

Section: Influence Of Proposed Methods On Cell Death Type Determinatimentioning

confidence: 99%

“…PI is routinely used to stain DNA and detect dead cells because of its ability to penetrate cell membrane only when it is permeable regardless of the mechanism of death [14]. On the other hand, Annexin V might be applied to distinguish apoptotic cells from necrotic ones based on changes in cell membrane caused by apoptosis [4]. During apoptosis phosphatidylserine (PS) is exposed at the cell surface and Annexin V has high affinity to PS.…”

Section: Fluorescent Stainingmentioning

confidence: 99%

“…During apoptosis phosphatidylserine (PS) is exposed at the cell surface and Annexin V has high affinity to PS. Annexin V cannot penetrate cell membrane and therefore it can be used for apoptosis detection [4].…”

Section: Fluorescent Stainingmentioning

confidence: 99%

“…Cell culture parameters, i.e. chosen variant of cell harvesting, either by mechanical scrapping or by enzymes and centrifugation may lead to false positive or false negative results [4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Methods in Studies of Cell Death Mechanisms

et al. 2018

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While studying the influence of ionizing radiation or certain chemical agents on cells, it is crucial to not only determine cytotoxicity, but also to follow cell death mechanisms. There are different methods to screen processes of cell death and still very important question remains unanswered about differences in results that could be caused by various experimental steps in procedures. Based on literature review two protocols of cell death determination were compared. First protocol regarded collecting cells floating in medium before trypsinization and following centrifugation of them. In the second protocol floating cells were discarded and attached ones were stained and fixed. In all experiments three different untreated cell lines (A172, DU145 as cancer cell lines and in comparison, fibroblasts (FB CCL 110), as a non-cancerous cell line) were used to test applied protocols. Cells were cultured and death processes were examined at different time points up to 120 h. Compared protocols showed statistically significant differences, especially in terms of necrosis, which was higher when included floating cells from culture medium and then centrifuging them. Therefore, presented results show importance of choosing a valid experimental procedure in case of evaluating cells viability and types of cell death pathways quantitatively.

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Section: Influence Of Proposed Methods On Cell Death Type Determinatimentioning

confidence: 99%

Section: Influence Of Proposed Methods On Cell Death Type Determinatimentioning

confidence: 99%

Section: Fluorescent Stainingmentioning

confidence: 99%

Section: Fluorescent Stainingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparison of Methods in Studies of Cell Death Mechanisms

et al. 2018

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Ropivacaine inhibits proliferation, invasion, migration and promotes apoptosis of papillary thyroid cancer cells via regulating ITGA2 expression

Qin

Liu

Wang³

2020

Drug Development Research

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The present study aimed to investigate the roles of Ropivacaine in papillary thyroid cancer (PTC) and identify the possible mechanisms. The expression of integrin alpha‐2 (ITGA2) in TC cell lines was tested using Western blotting and RT‐qPCR. Subsequently, the level of ITGA2 in human PTC cell line (TPC‐1) was measured following intervention with a series of concentrations of Ropivacaine. Then, cell counting kit‐8 (CCK‐8) assay and colony formation assay were executed for detecting proliferation of cells after transfection with ITGA2 pcDNA3.1. The expression of proliferation‐related protein was determined by Western blotting. Additionally, the abilities of TPC‐1 cell invasion and migration were examined using Transwell assay and scratch wound healing assay. Apoptosis of TPC‐1 cells was analyzed using TUNEL assay and the expressions of apoptosis‐related proteins were tested via West blotting. The results suggested that ITGA2 was highly expressed in TC cell lines, especially in TPC‐1 cells. Ropivacaine decreased the expression of ITGA2 in a dose‐dependent manner. Moreover, after treatment with Ropivacaine, cell proliferation was inhibited accompanied by changes of proliferation‐related protein expressions, which was reversed following co‐transfection with ITGA2 pcDNA3.1. Furthermore, Ropivacaine concentration‐dependently suppressed invasion and migration of TPC‐1 cells, whereas these inhibitory effects were attenuated after ITGA2 overexpression. Furthermore, apoptosis was promoted, coupled with a decrease of Bcl‐2 expression and increases of Bax, cleaved caspase‐3 and cleaved caspase‐9 expression, in Ropivacaine‐treated TPC‐1 cells, which was restored following ITGA2 overexpression. These findings demonstrated that Ropivacaine could suppress proliferation, invasion, migration, and accelerate apoptosis of PTC cells via regulating ITGA2 expression.

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Anesthesia Practices in Colorectal Cancer Surgery

Karaman

2020

Colon Polyps and Colorectal Cancer

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Effects of ropivacaine, bupivacaine and sufentanil in colon and pancreatic cancer cells in vitro

Cited by 50 publications

References 33 publications

Comparison of Methods in Studies of Cell Death Mechanisms

Comparison of Methods in Studies of Cell Death Mechanisms

Ropivacaine inhibits proliferation, invasion, migration and promotes apoptosis of papillary thyroid cancer cells via regulating ITGA2 expression

Anesthesia Practices in Colorectal Cancer Surgery

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