Effects of rifampicin on osteogenic differentiation and proliferation of human mesenchymal stem cells in the bone marrow

Zhang, Z; Wang, X; Luo, Fei; Yang, Hao; Hou, Tianyong; Zhou, Qiang; Dai, Fei; He, Qi; Xu, Jianzhong

doi:10.4238/2014.august.25.3

Cited by 9 publications

(6 citation statements)

References 9 publications

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“…A number of studies have shown that the osteogenic differentiation of stem cells was inhibited to varying degrees with the increase in rifampicin concentration. [22][23][24] The present study indicated that rifapentine was related to the inhibition of osteogenic differentiation of BMSCs. HA/β-TCP is a commonly used bone graft material in clinic with good biocompatibility and bone conduction properties, 25 and previous studies have found that HA/β-TCP scaffolds can promote osteogenic differentiation of BMSCs.…”

supporting

confidence: 51%

Rifapentine Polylactic Acid Sustained-Release Microsphere Complex for Spinal Tuberculosis Therapy: Preparation, in vitro and in vivo Studies

Wang¹,

Maimaitiaili²,

Wang³

et al. 2021

IDR

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Spinal tuberculosis has been a common clinical extrapulmonary tuberculosis in recent years. The general anti-tuberculosis drug treatment cycle is long, with unsatisfactory efficacy. This study focused on the preparation and evaluation of rifapentine polylactic acid sustained-release microsphere complex for spinal tuberculosis therapy. Methods: Rifapentine polylactic acid sustained-release microspheres (RPSMs) were prepared through the double emulsion solvent evaporation method, and RPSMs were combined with hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) composite material to obtain drugloaded, sustained-release complex. We evaluated the complex for dynamics of drug release and osteogenic ability using in vitro release test, alkaline phosphatase and alizarin red staining, real-time PCR and Western blot. A rabbit model of a spinal tuberculosis defect was established and repaired using HA/β-TCP or complex. The ability of anti-tuberculosis and tissue repair effects of the complex were evaluated through in vivo experiments. Results: The complex constructed of RPSMs and HA/β-TCP demonstrated a long drug release time, with no significant inhibition of cell osteogenic differentiation in vitro experiments. Postoperative macroscopic observation, immunohistochemical staining and Nilsson histological scores showed that the complex has good effects on the tissue repair. Moreover, the erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP), important indexes of inflammation, decreased to normal levels in the complex group. Conclusion:In vitro and in vivo experiments demonstrated that the complex constructed of RPSMs and HA/β-TCP effectively treated spinal tuberculosis. Therefore, the complex represents a promising approach for the treatment of spinal tuberculosis.

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supporting

confidence: 51%

Rifapentine Polylactic Acid Sustained-Release Microsphere Complex for Spinal Tuberculosis Therapy: Preparation, in vitro and in vivo Studies

Wang¹,

Maimaitiaili²,

Wang³

et al. 2021

IDR

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“…Another study demonstrated that rifampicin is cytotoxic to human bone marrow-derived MSCs at concentrations above 32 μg/mL and inhibited osteogenic diffe-rentiation potential of human bone marrow-derived MSCs in a concentration-dependent manner at concentrations ranging from 4-128 μg/mL. The collagen synthesis, mineralization effect, and expression levels of osteogenic genes in MSCs were inhibited to varying degrees with the increase in rifampicin concentration[15].…”

Section: Effects Of Various Antimicrobial Agents On Osteogenic Differmentioning

confidence: 99%

Effects of various antimicrobial agents on multi-directional differentiation potential of bone marrow-derived mesenchymal stem cells

Li¹,

Yue²

2019

WJSC

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Antimicrobial drugs of several classes play an important role in the treatment of bone and joint infections. In addition to fighting pathogenic microorganisms, the effects of drugs on local tissues and cells are also related to the course and prognosis of bone and joint infections. The multi-directional differentiation potential of bone marrow-derived mesenchymal stem cells (MSCs) is essential for tissue repair after local injury, which is directly related to the recovery of bone, cartilage, and medullary adipose tissue. Our previous studies and the literature indicate that certain antimicrobial agents can regulate the differentiation potential of bone marrow-derived MSCs. Here, in order to systematically analyze the effects of various antimicrobial drugs on local tissue regeneration, we comprehensively review the studies on the effects of these drugs on MSC differentiation, and classify them according to the three differentiation directions (osteogenesis, chondrogenesis, and adipogenesis). Our review demonstrates the specific effects of different antimicrobial agents on bone marrow-derived MSCs and the range of concentrations at which they work, and provides a basis for drug selection at different sites of infection.

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“…In these studies, rifamycin was shown to have toxic or inhibitory effects on osteoblast-like cells when used at concentrations exceeding a certain threshold. 27,28,29 While black cumin oil is well known for its various beneficial effects against several diseases 14 , its detailed effects on bone formation were examined in few studies. Wirries et al 30 reported a significant impact of TQ on proliferation, differentiation, and mineralization of cultured osteoblast cells.…”

Section: Resultsmentioning

confidence: 99%

A Study on the Use of Different Materials Combined With Allograft on Osseointegration and Bone Regeneration of Dental Implants With Coronal Defects in a Rabbit Model

Elmali¹,

ENİNANÇ

Yeler

et al. 2020

Cumhuriyet Dental Journal

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Objectives: To assess differential effects of different materials combined with allograft on bone-to-implant contact and newly formed bone formation in dental implants with coronal defects histomorphometrically. Materials and Methods: The study was conducted on 24 male New Zealand white rabbits. Dental implants (3.0 × 10 mm) were placed at the center of defects (9 mm diameter, 4 mm depth) created in the tibial bones of the rabbits. Graft (GF, n=8), graft + rifamycin (GR, n=8) and graft + black cumin oil via orogastric route (GB, n=8) were applied on the coronal aspects of the implants for 28 days. Undecalcified histomorphometric analyses were conducted on slides stained with toluidine blue. Results: Bone-to-implant contact was 46.57% ± 3.59% in the graft (GF), 67.12% ± 3.64% in the graft + rifamycin (GR) and 55.62% ± 4.37% in the graft + black cumin oil (GB) groups. The percentage of new bone formation at the defect area was 34.71% ± 4.11% in the graft, 55.37% ± 4.89% in the graft + rifamycin, and 45.75% ± 3.69% in the graft + black cumin oil groups. In terms of new bone formation and bone-to-implant contact, graft + rifamycin and graft + black cumin oil groups were significantly different from the graft group. The differences between the graft + rifamycin and graft + black cumin oil groups were also statistically significant. Conclusions: Allograft + rifamycin and orogastric black cumin oil were found to have positive effects on bone healing at sites with coronal defects. Rifamycin showed significantly greater favorable effects on bone-to-implant contact and new bone formation compared to black cumin oil.

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Effects of rifampicin on osteogenic differentiation and proliferation of human mesenchymal stem cells in the bone marrow

Cited by 9 publications

References 9 publications

Rifapentine Polylactic Acid Sustained-Release Microsphere Complex for Spinal Tuberculosis Therapy: Preparation, in vitro and in vivo Studies

Rifapentine Polylactic Acid Sustained-Release Microsphere Complex for Spinal Tuberculosis Therapy: Preparation, in vitro and in vivo Studies

Effects of various antimicrobial agents on multi-directional differentiation potential of bone marrow-derived mesenchymal stem cells

A Study on the Use of Different Materials Combined With Allograft on Osseointegration and Bone Regeneration of Dental Implants With Coronal Defects in a Rabbit Model

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