Effects of rice straw structure on chaetoglobosin A production by Chaetomium globosum CGMCC 6882

Wang, Zichao; Cui, Jingwen; Gao, Wenshuo; Yang, Qing; Chen, Lingzi; Yang, Libo; Sun, Qi; Zhang, Huiru

doi:10.1016/j.ijbiomac.2019.10.132

Cited by 9 publications

(4 citation statements)

References 36 publications

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“…Biological, chemical or physical pretreatment of straw is usually used to separate polymers and expose soluble components ( Mussoline et al, 2013 ; Wang Z. C. et al, 2020 ). With time passing, the dominant components of the corn straw constantly varied during the degradation process.…”

Section: Resultsmentioning

confidence: 99%

Characterizing corn-straw-degrading actinomycetes and evaluating application efficiency in straw-returning experiments

Gong¹,

Yu²,

Hao³

et al. 2022

Front. Microbiol.

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Corn straw is an abundant lignocellulose resource and by-product of agricultural production. With the continuous increase in agricultural development, the output of corn straw is also increasing significantly. However, the inappropriate disposal of straw results in wasting of resources, and also causes a serious ecological crisis. Screening microorganisms with the capacity to degrade straw and understanding their mechanism of action is an efficient approach to solve such problems. For this purpose, our research group isolated three actinomycete strains with efficient lignocellulose degradation ability from soil in the cold region of China: Streptomyces sp. G1T, Streptomyces sp. G2T and Streptomyces sp. G3T. Their microbial properties and taxonomic status were assessed to improve our understanding of these strains. The three strains showed typical characteristics of the genus Streptomyces, and likely represent three different species. Genome functional annotation indicated that most of their genes were related to functions like carbohydrate transport and metabolism. In addition, a similar phenomenon also appeared in the COG and CAZyme analyses, with a large number of genes encoding carbohydrate-related hydrolases, such as cellulase, glycosidase and endoglucanase, which could effectively destroy the structure of lignocellulose in corn straw. This unambiguously demonstrated the potential of the three microorganisms to hydrolyze macromolecular polysaccharides at the molecular level. In addition, in the straw-returning test, the decomposing consortium composed of the three Streptomyces isolates (G123) effectively destroyed the recalcitrant bonds between the various components of straw, and significantly reduced the content of active components in corn straw. Furthermore, microbial diversity analysis indicated that the relative abundance of Proteobacteria, reportedly associated with soil antibiotic resistance and antibiotic degradation, was significantly improved with straw returning at both tested time points. The microbial diversity of each treatment was also dramatically changed by supplementing with G123. Taken together, G123 has important biological potential and should be further studied, which will provide new insights and strategies for appropriate treatment of corn straw.

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Section: Resultsmentioning

confidence: 99%

Characterizing corn-straw-degrading actinomycetes and evaluating application efficiency in straw-returning experiments

Gong¹,

Yu²,

Hao³

et al. 2022

Front. Microbiol.

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“…SEM and FT‐IR analyses revealed that both C. globosum and OEX13 could degrade the cornstalk substrate and produce chaetoglobosin A. The chaetoglobosin A production by OEX13 was significantly improved by 475.94% (from 40.32 to 191.90 mg/L), and a further enhancement in the chaetoglobosin A yield could possibly be achieved by straw pretreatment and humidity adjustment (Wang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

New insight into the production improvement and resource generation of chaetoglobosin A in Chaetomium globosum

Zhao

Lin

Cheng

et al. 2022

Microbial Biotechnology

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Chaetoglobosin A is a complex macrocyclic alkaloid with potent antimycotic, antiparasitic and antitumor properties. However, the low output and high cost of chaetoglobosin A biosynthesis have hampered the application and commercialization of chaetoglobosin A in agriculture and biomedicine. Here, the CgMfs1 gene, which encodes the major facilitator superfamily secondary transporter, was identified based on bioinformatics analysis, and an intensive study of its effects on chaetoglobosin A biosynthesis and secretion was performed using CgMfs1‐silencing and CgMfs1‐overexpression strategies. Inactivation of CgMfs1 caused a notable decrease in chaetoglobosin A yield from 58.66 mg/L to 19.95 mg/L (MFS1–3) and 17.13 mg/L (MFS1–4). The use of an efficient expression plasmid in Chaetomium globosum W7 to generate the overexpression mutant OEX13 resulted in the highest chaetoglobosin A increase to 298.77 mg/L. Interestingly, the transcription level of the polyketide synthase gene significantly fluctuated with the change in CgMfs1, confirming that the predicted efflux gene CgMfs1 could play a crucial role in chaetoglobosin A transportation. Effective efflux of chaetoglobosin A could possibly alleviate feedback inhibition, resulting in notable increase in the expression of the polyketide synthase gene. Furthermore, we utilized cornstalk as the fermentation substrate to produce chaetoglobosin A, and scanning electron microscopy and Fourier transform‐infrared spectroscopy revealed that the strain OEX13 could well degrade cornstalk, presenting significant increases in the chaetoglobosin A yield, when compared with that produced by the wild‐type strain (from 40.32 to 191.90 mg/L). Thus, this research provides a novel analogous engineering strategy for the construction of high‐yielding strain and offers new insight into large‐scale chaetoglobosin A production.

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“…Chaetoglobosin A is an alkaloid compound with anticancer properties and is produced by fungi. Extrusion of lignocellulose in the presence of glycerol and aqueous NaHCO 3 solution resulted in accelerated degradation and also reduced the particle size and crystallinity of the components but effectively increased the yield of chaetoglobosin A isolation from rice straw by C. globosum CGMCC 6882 [260].…”

Section: Extrusionmentioning

confidence: 99%

Mechanochemical Degradation of Biopolymers

Jicsinszky,

Bucciol,

Chaji

et al. 2023

Molecules

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Mechanochemical treatment of various organic molecules is an emerging technology of green processes in biofuel, fine chemicals, or food production. Many biopolymers are involved in isolating, derivating, or modifying molecules of natural origin. Mechanochemistry provides a powerful tool to achieve these goals, but the unintentional modification of biopolymers by mechanochemical manipulation is not always obvious or even detectable. Although modeling molecular changes caused by mechanical stresses in cavitation and grinding processes is feasible in small model compounds, simulation of extrusion processes primarily relies on phenomenological approaches that allow only tool- and material-specific conclusions. The development of analytical and computational techniques allows for the inline and real-time control of parameters in various mechanochemical processes. Using artificial intelligence to analyze process parameters and product characteristics can significantly improve production optimization. We aim to review the processes and consequences of possible chemical, physicochemical, and structural changes.

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Effects of rice straw structure on chaetoglobosin A production by Chaetomium globosum CGMCC 6882

Cited by 9 publications

References 36 publications

Characterizing corn-straw-degrading actinomycetes and evaluating application efficiency in straw-returning experiments

Characterizing corn-straw-degrading actinomycetes and evaluating application efficiency in straw-returning experiments

New insight into the production improvement and resource generation of chaetoglobosin A in Chaetomium globosum

Mechanochemical Degradation of Biopolymers

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