Effects of rhamnolipids and shear on initial attachment of Pseudomonas aeruginosa PAO1 in glass flow chambers

Abstract

As compared to static systems, the flow chamber systems significantly improved the reproducibility of initial attachment results. Flow chamber systems were more suitable for experimental investigations of bacterial attachment to surfaces. Rhamnolipids were found to be potent antifoulants for PAO1 attachment on glass. The initial cell attachment increased with increasing shear at the very low shear range (up to 3.5-5.0 mN/m(2)), but the attachment could be minimized with further increase of the shear.

Biosurfactants and Bioemulsifiers Biomedical and Related Applications – Present Status and Future Potentials

“…The purified glycolipid disrupted the biofilm formation under dynamic conditions and the biofilm-forming capacity of both mixed culture and individual human and fish pathogenic strains was significantly inhibited at 30 mg/mL glycolipid. Raya et al, (2010) analyzed the effects of rhamnolipids and shear on initial attachment of Pseudomonas aeruginosa PAO1 in glass flow chambers. The presence of rhamnolipids significantly reduced the initial attachment of PAO1, even at the low concentration of 13 mg/L.…”

Role of Biofilm Roughness and Hydrodynamic Conditions in Legionella pneumophila Adhesion to and Detachment from Simulated Drinking Water Biofilms

“…In addition to biofilm roughness, hydrodynamic conditions were also shown to influence cell adhesion to and detachment from multiple surfaces. 25–28 High shear stress caused by high flow velocity prevented cell adhesion onto the clean and smooth surfaces, 25,27 and enhanced detachment of the adhered biomass. 25,28,29 Nevertheless, for heterogeneous rough biofilm surfaces, local hydrodynamics could be disturbed by the surface asperities.…”

Effect of surfactants on the biofilm of Rhodococcus erythropolis, a potent degrader of aromatic pollutants

“…Also, direct interaction of surfactants with cell envelopes can contribute to the change of cell surface hydrophobicity [8]. By contrast, numerous published findings demonstrate the anti-adhesive effects of rhamnolipids [6,[8][9][10][11][12]. Rhamnolipids emerged as a powerful tool for biofilm destruction of the respiratory pathogen Bordetella bronchiseptica [13].…”