Effects of Resveratrol on Migration and Proliferation in HT-29 Colon Cancer Cells

Abstract

1) Resveratrol, natural polyphenol in grapes and red wine, is known to have the anti-proliferatory and anti-angiogenic effects in various cancer cells. In this study, we have investigated the effects of resveratrol in HT-29 colon cancer cells. Treatment of resveratrol in different concentrations and time inhibited proliferation of HT-29 colon cancer cells. We explored the effects of resveratrol on HT-29 colon cancer cell motility using a wound healing assay. In the absence of the resveratrol, the HT-29 cells are migrated along the edges of the wound and showed a large-scale migration, whereas dose-and time-dependent inhibition of cell flattening and spreading was observed in the presence of resveratrol. Resveratrol inhibited MMP-9 in a dose-and time-dependent on HT-29 colon cancer cells by Western blotting. In addition, resveratrol increased AMPK activity and decreased COX-2, VASP and VEGF expression. Treatment of compound C inhibited AMPK activity, however, the expression of VASP and COX-2 increased thus, COX-2 and VASP are modulated by AMPK. However treatment of celecoxib could not control AMPK activity but decreased VEGF expression. We suggest that resveratrol inhibits cell proliferation and migration through activation of

Apoptotic effects of extract from Cnidium monnieri (L.) Cusson by adenosine monosphosphate-activated protein kinase-independent pathway in HCT116 colon cancer cells

“…Cancer is caused by the abnormal proliferation of cells, and interference with cell proliferation as well as induction of cancer cell death have been widely employed in the treatment of cancer (6). Colon cancer is of particular interest in South Korea, as its prevalence has increased with the adaptation of Western eating habits (1,2). Previous studies have shown that various plant extracts have anti-cancer effects and cause apoptosis of HCT116 colon cancer cells (4,(26)(27)(28).…”

“…results indicated that CME exerts cytotoxic effects and induces apoptosis via the downregulation of Akt. p-AMPK and p-Akt are upstream regulators that trigger apoptosis through modulation of individual signaling pathways (2,12). To investigate whether CME downregulates Akt through the activation of AMPK or through direct inhibition, signaling proteins associated with AMPK-and Akt-mediated apoptosis were assessed after treatment CME alone or following pre-treatment with AMPK and Akt inhibitors.…”

“…Colon cancer is a common malignancy with a high incidence worldwide, which is increasing each year in South Korea as the population ages and adapts to westernized eating habits (1)(2)(3). Most attention is focused on post-operative cancer metastasis, as the post-operative survival rate of colon cancer is only 50% (4).…”

Apoptotic Effects and Cell Cycle Arrest Effects of Extracts from Cnidium monnieri (L.) Cusson through Regulating Akt/mTOR/GSK-3β Signaling Pathways in HCT116 Colon Cancer Cells

“…대장암은 전 세계적으로 발병률이 높은 악성종양 중의 하나 로, 국내에서도 서구화된 식습관으로 인하여 매년 증가하고 있다 [21,25,27]. 대장암은 주로 암을 절제하는 외과적 수술로 치료가 이루어지며, 암의 병기(staging)에 따라 항암제 또는 방사선치료가 병행된다.…”